Description:
Description:NotchPathwayReporterkitisdesignedformonitoringtheactivityofNotchsignalingpathwayintheculturedcells.Thekitcontainsthetransfection-readyNotch1NICDexpressionvectorandCSL(CBF1/RBP-J?)luciferasereportervector,whichisaNotchpathway-responsivereporter.ThisreportercontainsafireflyluciferasegeneunderthecontrolofmultimerizedCSLresponsiveelementupstreamofaminimalpromoter.TheCSL(CBF1/RBP-J?)reporterispremixedwithconstitutivelyexpressingRenilla(seapansy)luciferasevector,whichservesasaninternalpositivecontrolfortransfectionefficiency.Thekitalsoincludesanon-inducIBLefireflyluciferasevectorpremixedwithconstitutively-expressingRenillaluciferasevectorasanegativecontrol.Thenoninducibleluciferasevectorcontainsafireflyluciferasegeneunderthecontrolofaminimalpromoter,butwithoutanyadditionalresponseelements.Thenegativecontroliscriticalfordeterminingpathwayspecificeffectsandbackgroundluciferaseactivity.Application:MonitorNotchsignalingpathwayactivity,ScreenactivatorsorinhibitorsofNotchsignalingpathway,StudyeffectsofRNAiorgeneoverexpressionontheactivityofNotchpathway.Components:CSL(CBF1/RBPJ?)luciferasereportervector+constitutivelyexpressingRenillaluciferasevector,500ul;NegativeControlNon-inducibleluciferasevector+constitutivelyexpressingRenillaluciferasevector,500ul;-20°CNotch1NICDExpressionvector,250ul;NegativeControlExpressionvector,250ul.References:1.LuFMetal.(1996).Proc.Natl.Acad.Sci.USA93(11):5663-5667.2.MoelleringREetal.(2009).Nature462(7270):182-188.Format:assaykit
ScientificCategory:CellSignalingPathway
AdditionalInformation:
| Name | NotchReporterAssayKit(cAMP/PKAPathway) |
|---|---|
| RelatedProductNames | Notch,PKA,cAMP,luciferase |
| Format | AssayKit |
| Storage | -20°C |
| IntendedUse | ResearchUseOnly |

