Product Description
Overview
The HyStem-IRG QuickSet Kit is composed of Glycosil® (thiolmodified hyaluronic acid), Gelin-S® (thiol-modified gelatin), PEGCure (PEG-norbornene) and Irgacure 2959 Photoinitiator for photoinitiation. A transparent hydrogel forms after contents are mixed and exposed to UV light (365 nm). All vials are packaged as sterile lyophilized solids that are blanketed by argon and under a slight vacuum.
As one of the first hyaluronic acid-based light-controlled hydrogels, the HyStem®-IRGQuickSet Kit supports 3D cell culture for use in tissue engineering and small scale bioprinting applications. The HyStem®-IRGQuickSet Kitprovides increased temporal and spatial control making it ideal for micro-scale bioprinting. Gelation occurs after 30-60 seconds when exposed to ultraviolet light as compared to ~30 minutes using other hydrogel formulations.
Features
- Enables faster, flexible gelation times
- Controlled by UV light
- Optimized for micro-scale bioprinting applications
- Maintains standard characteristics of other HyStem hydrogels
The HyStem®UV QuickSet kit is composed of HyStem (thiol-modified hyaluronic acid), Gelin-S®(thiol-modified gelatin), UVlink™ (PEG-norbornene), Irgacure 2959 for photoinitiation, and DG water. A transparent hydrogel forms when contents are mixed and exposed to UV light. All vials are packaged as sterile lyophilized solids that are blanketed by argon and under a slight vacuum.
Directions for Use
INSTRUCTIONS FOR USE (Download the full directions for use PDF here)
The Irgacure solution is prepared by dissolving the lyophilized solid in DG Water. The Glycosil, Gelin-S, and PEGCure are reconstituted with the Photoinitiator solution. A 7.5 mL hydrogel at 1% (w/v) solution is produced when all reconstituted materials are mixed.
HyStem hydrogels (3 x 2.5 mL = 7.5 mL) should be prepared as follows:
- Allow Glycosil, Gelin-S, PEGCure, irgacure, and DG Water vials to come to room temprerature.
- Under aseptic conditions, use a syringe to add 10.0 mL of DG Water to the Irgacure Photoinitiator vial. Shake or vortex the vial at 37°C for 30 minutes or until fully dissolved.
- Add 1.0 mL of the reconstituted Photoinitiator to the Glycosil vial. Add 1.0 mL of the Photoinitiator solution to the Gelin-S vial.
- Place both vials horizontally on a rocker or shaker. Shake vials at 37°C for 30 minutes or until fully dissolved. It may take up to 60 minutes for the solids to fully dissolve.
Solutions should be clear and slightly viscous.
- Add 0.5 mL of Photoinitiator to the PEGCure vial. Place on a shaker and mix at 37°C for approximately 10 minutes.
- Combine the Glycosil, Gelin-S, and PEGCure solutions and mix well.
- Pipette solution into desired format (i.e. 96 well plate). Using a hand-held UV light source, expose the gel to the UV light (wavelength 365nm) until the gel reaches the desired stiffness. Gelation will occur between 15 seconds and 1 minute. Results may vary depending on the UV source manufacturer and/or plate design
Note: Gelation time and gel stiffness can be adjusted by varying the concentration of Glycosil, GelinS, or UVlink.
Note: Each kit component has been manufactured under aseptic conditions and tested for bacteria and fungus.
Product Q & A
Globular particles less than 75 kDa should be able to freely diffuse through a HyStem hydrogel.
When reconstituted using DG water, the pH of each HyStem component will be approximately 7.4-7.6.
One year from the date of receipt, if stored properly.
Any sterile, deionized, degassed water can be substituted for reconstitution. However, in order to ensure accurate and predictable dissolution and gelation times, our DG Water is highly recommended, as it is degassed, blanketed in argon, and has undergone validation testing with each HyStem component.
Gelin-S provides cellular attachment sites when incorporated in the hydrogel. Gelin-S is thiol-modified, denatured collagen I, derived from either bovine or porcine sources. Gelin-S is included in all HyStem-C and HyStem-HP kits.
Gelin-S has been thiol-modified in the same manner as the hyaluronan in Glycosil (or Heprasil), so that it covalently crosslinks with the Extralink in the HyStem hydrogels.
Yes. Peptides that contain a cysteine residue can be used. The cysteine residue must be present for the peptide to be covalently bonded to the hydrogel substrate.
Yes. ECM proteins, such as laminin, collagen, fibronectin, or vitronectin can be non-covalently incorporated into the hydrogel prior to crosslinking.
HyStem hydrogels and sponges differ in hydration and homogeneity. HyStem sponges are typically polymerized hydrogels that are subsequently freeze-dried. The resulting sponge is a fibrous, mesh network with pores and niches that enable cells to infiltrate and adhere. A true HyStem hydrogel is an encapsulating liquid that polymerizes around suspended cells in culture.
No. The compliance of the hydrogels is set by the amount of Extralink crosslinker added, the concentration of Glycosil (or Heprasil) and Gelin-S used, and the ratio of Glycosil (or Heprasil) to Gelin-S. Once this chemical structure of the hydrogel is fixed, it is not altered by prolonged exposure to cell culture medium.
HyStem sponges can be terminally sterilized by E-beam. HyStem hydrogels have not yet been validated for use with E-beam sterilization methods. HyStem hydrogels are not terminally sterilized by gamma irradiation.
Gelation time is affected by multiple aspects of the gel’s composition.One way to change the gelation time of a hydrogel is to vary the amount of crosslinker used. Gels with a lower amount of Extralink crosslinker will have a longer gelation time than those with a higher amount of crosslinker. Changing the amount of crosslinker will produce slight changes in gelation time.Gelation time can be dramatically changed by varying the Glycosil (or Heprasil) and Gelin-S concentrations. Concentrated solutions of Glycosil (or Heprasil) and Gelin-S will create a solution with a much shorter gelation time. This can easily be done by reconstituting the components in a smaller volume of DG Water. Alternatively, diluting these components in larger volumes of DG Water will dramatically increase the total time to form the hydrogel.
HyStem Hydrogels are virtually transparent and should not interfere with microscopy.
HyStem hydrogels may generate mild inflammation as part of the body’s natural healing process in response to injury. HyStem hydrogels do not trigger immune response when used in vivo. (These products are not for human use)
HyStem is degraded in vivo by matrix metalloproteinases (collagenases) and hyaluronidases.
Trypsin, Dipase, collagenase, and hyaluronidase have been used to help detach cells from the surface or from within HyStem hydrogels.
In general, the pore size for HyStem-C and HyStem-HP hydrogels is ~17 nm.
Product Applications
General Notes for Bioprinting with HyStem®-IRG QuickSet Kit:
The following guidelines were developed for a modified Bioforce Nano eNabler™ and exact requirements and parameters will vary between platforms and applications. While other platforms have not been evaluated, any system capable of liquid handling and amenable to UV exposure should be easily adaptable for the HyStem®-IRG QuickSet matrix.
• High relative humidity (>90%) is required in the printing chamber to prevent desiccation of printed constructs.
• If printing live cells, verify that the surface patterning tool or print head is large enough to accommodate cells (approximately 50-100 µm).
• Where possible, UV/ozone treatment of printing tools to enhance hydrophilic nature of the printing channel materials can improve consistency in printing.
• The matrix is a clear liquid with minimally increased viscosity compared to water at room temperature. Upon exposure to 365nm UV light, rapid polymerization occurs resulting in a viscoelastic solid. A 4W handheld UV lamp held approximately 2 cm from the surface of the gel is sufficient to polymerize a gel in as little as 15 seconds.
• Conducting a small pilot study to determine the appropriate exposure time for a given application is strongly recommended as matrix stiffness is directly correlated to exposure time and can impact survivability, proliferation, and differentiation.
Note: The HyStem®-IRG QuickSet Kit has not been thoroughly evaluated for thick, multilayer constructs.
Product Certificate of Analysis
Safety and Documentation
Certificate of Origin
Safety Data Sheet
Product Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
美国AdvancedBioMatrix（简称ABM） www.advancedbiomatrix.comAdvancedBioMatrix（简称ABM）是美国一家著名的生物公司，获得了AllerganInc的授权(Allergan用25年时间不断完善胶原蛋白相关的产品的生产工艺)，将Allergan的专业和技术用于蛋白生产与检测，致力于为组织工程、细胞分析及细胞增殖等研究领域提供优质稳定的产品。AdvancedBioMatrix不断丰富已有产品线，目前可为三维细胞培养提供各种胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、不同粘度与分子量的透明质酸以及低代成纤维细胞等。在美国全部产品授权Sigma销售。AdvancedBioMatrix是组织培养，细胞分析和细胞增殖三维（3D）应用的生命科学领域的领导者。我们的产品被公认为纯度，功能性和一致性的标准。我们在生产，分离，纯化，冷冻干燥，细胞培养和蛋白质测试，粘附肽，附着因子，底物刚性和其他3D矩阵产品方面拥有丰富的专业知识。我们的专业技术和知识正在被用来确保我们的产品质量最高，批次之间一致且易于为我们的研究客户使用。
美国AdvancedBioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。AdvancedBioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3Dmatrix 产品开发方面有着丰富的经验。AdvancedBioMatrix的研发经验和专业知识确保其产品可达到最佳质量，并保证产品之间一致性，方便研究客户使用。以下为AdvancedBioMatrix3DMatrices 产品竞争优势：1. 提供高纯度和成分确定的胞外基质；2. 超过1000余篇文献引用PureCol产品，品质非常均一；3. 在3D培养基领域可提供最全面的产品线；4. 唯一可提供特异性刚性有机硅基板的公司（CytoSoft）;5. 唯一可提供可溶性丝纤蛋白的供应商（可运用于多种3D培养）；6. 如果客户首次接触3D胶原凝胶，AdvancedBioMatrix还是唯一的预制胶原蛋白(PureColEZGel)供应商；
以下产品为AdvancedBioMatrix全球畅销品：1.PureCol 牛源I型胶原蛋白 3mg/ml#5005-100ML2.Nutragen牛源I型胶原蛋白 6mg/ml#5010-50ML3.FibriCol 牛源I型胶原蛋白 10mg/ml#5133-20ML4.VitroCol 人源I型胶原蛋白 #5007-20ML5. 弹性蛋白原 #5052-1MG6.ECMSelectArraykitUltra-36#5170-1EA7.CytoSoft（刚性可变的基底，AdvancedBioMatrix最新添加产品5190-7EA）8. 人III型胶原蛋白 #5021-10MG9. 人IV型胶原蛋白 #5022-5MG10.SilkFibroin溶液 #5154-20ML11.Fibronectin#5080-5MG12.Vitronectin#5051-0.1MG
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的试剂：免疫吸附剂、结合物和酶的底物。
完整的ELISA试剂盒包含以下各组分：
（1）已包被抗原或抗体的固相载体（免疫吸附剂）；
（2）酶标记的抗原或抗体（结合物）；
（3）酶的底物；
（4）阴性对照品和阳性对照品（定性测定中），参考标准品和控制血清（定量测定
中）；
（5）结合物及标本的稀释液；
（6）洗涤液；
（7）酶反应终止液。
Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法。
但是在选择的时候,也需要注意其他的一些问题。
1、采用何种原料和抗体,是否高效、灵敏、特异
2、规范包被操作,吸附是否均匀
3、重复性、可靠性
6、是否提供技术服务
7、适用于血浆、血清、组织匀浆液、细胞培养上清液、尿液等多种类型的样本
8、可检测动物类型是否丰富
9、可检测指标是否齐全
elisa试剂盒 就查下博欧特生物
使用方法：
1、 血清：操作过程中避免任何细胞刺激。使用不含热原和内毒素的试管。收集血液后，1000×g离心10分钟将血红细胞迅速小心地分离。
2、 血浆：EDTA、柠檬酸盐、肝素血浆可用于检测。1000×g离心30分钟去除颗粒。
3、 细胞上清液：1000×g离心10分钟去除颗粒和聚合物。
4、 组织匀浆：将组织加入适量生理盐水捣碎。1000×g离心10分钟，取上清液。
5、 保存：如果样品不立即使用，应将其分成小部分-70℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。
1、是不是获得SCI文献引用；
2、试剂盒是不是具备高灵敏，质量好的；
3、售后服务完全，提供代测服务；
4、后期实验提供全程技术支持！
