Description
Formostsurfacereactiveliposomeformulations,thereactivelipidisincorporatedintotheliposomesandtheligand,proteinorpeptideisconjugateddirectlytotheliposomesthatcontainreactivelipids.However,thisstrategymightnotworkifthereactivelipidispronetohydrolysissuchasmaleimideorNHSlipid.Inordertogetaroundthisissue,theliposomesaremadewithoutthePEGylated(reactiveandnon-reactive)lipids.Thescientistwhoisplanningtousethekitwillconjugatetheligand,proteinorpeptidetothereactivelipid.Next,thebufferisaddedtothelipid-conjugatedligandandmicellesolutionisformed.AnothermicellesolutionisformedafterthePEGylatednon-reactivelipidishydratedwiththebuffer.Thetwomicellesolutionsaremixedandincubatedtogetheratatemperatureabovetheliquid-to-gelphasetransitiontemperatureoftheHSPC(saturatedmatrixlipid).ThePEGylatedlipidsinthemicellesinsertthemselveswithahighefficiency(above80%)totheliposomesandformtheimmunoliposomes.
Surfacereactivedoxorubicinliposomes(usingvarioustypesofchemistries)suitableforconjugationofvariousantibodies,proteins,peptidesandligandsarealsoavailable.Formoreinformationaboutsurfacereactivedoxorubicinliposomes(Immundox®)seehere.
FormulationInformation
Doxosome™Control-DoxorubicinLiposomes(Non-PEGylated)forPost-insertion
| LipidComposition | Concentration(mg/ml) | Concentration(mM) | MolarRatioPercentage |
|---|---|---|---|
| Total | 15.33mg/ml | 24.61mM | 100 |
| HydrogenatedSoyPC | 11.5 | 14.7 | 60 |
| Cholesterol | 3.83 | 9.91 | 40 |
| BuffersandLiposomeSize | Specification |
|---|---|
| InsideBuffer | AmmoniumSulfate |
| OutsideBuffer | PhosphateBufferedSaline |
| pH | 7.4 |
| LiposomeSize | 100nm |
LiposomeParticleCalculator
Doxorubicinliposomesareunilamellarandsizedto100nm.Themolarconcentrationofliposomeis24.61mM.Byhaving liposomediameter(nm)andlipidconcentration(µM),youcancalculatethetotalnumberofthelipidsinoneliposomeandthenumberoftheliposomesinonemilliliteroftheliposomesolution.Tousethecalculatorclick here.
Appearance
Doxosome™Controlisawhitetranslucentliquidmadeofnanosizeunilamellarliposomes.Usually,duetothesmallsizeofliposomes,nosettlingwilloccurinthebottomofthevial.Theliposomesarepackagedinanambervial.
EducationalVideo
Ordering/ShippingInformation
- Allliposomebasedformulationsareshippedonblueiceat4°Cininsulatedpackagesusingovernightshippingorinternationalexpressshipping.
- LiposomesshouldNEVERbefrozen.Icecrystalsthatforminthelipidmembranecanrupturethemembrane,changethesizeoftheliposomesandcausetheencapsulateddrugtoleakout.Liposomesinliquidformshouldalwaysbekeptintherefrigerator.
- ClientswhoorderfromoutsideoftheUnitedStatesofAmericaareresponsIBLefortheirgovernmentimporttaxesandcustomspaperwork.EncapsulaNanoSciencesisNOTresponsibleforimportationfeestocountriesoutsideoftheUnitedStatesofAmerica.
- WestronglyencouragetheclientsinJapan,Korea,TaiwanandChinatoorderviaadistributor.Toughcustomsclearanceregulationsinthesecountrieswillcausedelayincustomclearanceoftheseperishableformulationsifordereddirectlythroughus.Distributorscaneasilyclearthepackagesfromcustoms.Toseethelistofthedistributorsclickhere.
- ClientsorderingfromuniversitiesandresearchinstitutesinAustraliashouldkeepinmindthattheliposomeformulationsaremadefromsyntheticmaterialandtheformulationsdonotrequirea“permittoimportquarantinematerial”.LiposomesareNOTBIOLOGicalproducts.
- Ifyouwouldlikeyourinstitute’sFedExorDHLaccounttobechargedforshipping,thenpleaseprovidetheaccountnumberatthetimeofordering.
- EncapsulaNanoScienceshasnocontroloverdelaysduetoinclementweatherorcustomsclearancedelays.YouwillreceiveaFedExorDHLtrackingnumberonceyourorderisconfirmed.ContactFedExorDHLinadvanceandmakesurethatthepaperworkforcustomsisdoneontime. AllsubsequentshippinginquiriesshouldbedirectedtoFederalExpressorDHL.
StorageandShelfLife
Storage
Doxosome™productsshouldalwaysbestoredatinthedarkat4°C,exceptwhenbroughttoroomtemperatureforbriefperiodspriortoanimaldosing.DONOTFREEZE.IfthesUSPensionisfrozen,theencapsulateddrugcanbereleasedfromtheliposomesthuslimitingitseffectiveness.Inaddition,thesizeoftheliposomeswillalsochangeuponfreezingandthawing.
ShelfLife
Doxosome™ismadeondailybasis.Thebatchthatisshippedismanufacturedonthesameday.Itisadvisedtousetheproductswithin4monthsofthemanufacturingdate.
ReferencesandbackgroundreADIng
1.HermansonGT.Bioconjugatetechniques.Academicpress;2013Jul25.
2.TorchilinV,WeissigV,editors.Liposomes:apracticalapproach.OxfordUniversityPress;2003Jun5.
3.GrabarekZ,GergelyJ.Zero-lengthcrosslinkingprocedurewiththeuseofactiveesters.Analyticalbiochemistry.1990Feb15;185(1):131-5.
4.YanL,CraytonSH,ThawaniJP,AmirshaghaghiA,TsourkasA,ChengZ.ApH‐ResponsiveDrug‐DeliveryPlatformBasedonGlycolChitosan–CoatedLiposomes.Small.2015Oct1;11(37):4870-4.
5. Silva-LópezEI,EdensLE,BardenAO,KellerDJ,BrozikJA.ConditionsforliposomeadsorptionandbilayerformationonBSApassivatedsolidsupports.Chemistryandphysicsoflipids.2014Oct31;183:91-9.
6.HazraM,SinghSK,andRayS.SurfaceModificationofLiposomalVaccinesbyPeptideConjugation.JournalofPharmaSciTech,2011;1(1):41-47.
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1、是不是获得SCI文献引用；
2、试剂盒是不是具备高灵敏，质量好的；
3、售后服务完全，提供代测服务；
4、后期实验提供全程技术支持！
的试剂：免疫吸附剂、结合物和酶的底物。
完整的ELISA试剂盒包含以下各组分：
（1）已包被抗原或抗体的固相载体（免疫吸附剂）；
（2）酶标记的抗原或抗体（结合物）；
（3）酶的底物；
（4）阴性对照品和阳性对照品（定性测定中），参考标准品和控制血清（定量测定
中）；
（5）结合物及标本的稀释液；
（6）洗涤液；
（7）酶反应终止液。
elisa试剂盒回收率是反应待测物在样品分析过程中的损失的程度，损失越少，回收率越高，如果作标液1PPM，就是1毫克/升，而作出标准数据为0.99毫克/升，就是说你的回收率是99%，这个与真实成分有密切的关系，说明方法的准确度。比如水中总无机氯含量测定，样品水中含有无机氯20mg/L，取100mL被测水样品，加入0.1mL浓度为10mg/mL的含无机氯标准样品，测定时忽略体积变化，如果测定出样品中无机氯为2.98mg/L，则认为回收率为99%。向左转|向右转
第二步、明确编码该蛋白的基因与其它哪些物种同源性最好
第三步、寻找检测这些物种蛋白的试剂盒
第四步、咨询商家ELISA是试剂盒使用的抗体类型:多抗还是单抗? 单抗是针对什么抗原决定簇的?
第五步、 做出自己的判断该买哪个试剂盒，或者实在没有可以找公司进行订制实验
1、采用何种原料和抗体,是否高效、灵敏、特异
2、规范包被操作,吸附是否均匀
3、重复性、可靠性
6、是否提供技术服务
7、适用于血浆、血清、组织匀浆液、细胞培养上清液、尿液等多种类型的样本
8、可检测动物类型是否丰富
9、可检测指标是否齐全
