
Solution for Easy Concentration
- High water absorbent capacity
- Easy to use with any MWCO dialysis membrane
- Easy to concentrate small molecular weight molecules
- Easy to remove
- Cost effective
- Convenient for sample concentration with Float-A-Lyzer G2
Spectra/Gel makes the concentration of protein sample solutions quick and efficient. Made of polyacrylate-polyalcohol it has the property of expanding as it absorbs water. The high molecular weight polymer can be used with dialysis membranes of molecular weight cut off"s (MWCO) from 100 to 1,000,000 Daltons without contaminating the sample. After dialysis, simply remove the dialysis tubing containing the sample and coat it with Spectra/Gel. Water and smaller molecules diffuse through the membrane and are absorbed by Spectra/Gel Absorbent. Concentration continues unattended until a certain volume of water is removed from the sample. The insoluble characteristic makes Spectra/Gel easy to remove after absorbancy.
The length of time needed for concentration will depend on the final volume, the MWCO, the surface to volume ratio of the membrane tubing and the amount of Spectra/Gel Absorbent. After dialysis in a Spectra/Por Float-A-Lyzer G2 with a 20 kD MWCO, the volume can be reduced from 5 mL to 0.5 mL in 60 minutes with 100 grams of the Spectra/Gel Absorbent.
Easy-to-use dialysis products such as Spectra/Por Float-A-Lyzer G2 can be used with Spectra/Gel to concentrate samples.
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1、是不是获得SCI文献引用;
2、试剂盒是不是具备高灵敏,质量好的;
3、售后服务完全,提供代测服务;
4、后期实验提供全程技术支持!
2、规范包被操作,吸附是否均匀
3、重复性、可靠性
6、是否提供技术
7、适用于血浆、血清、组织匀浆液、细胞培养上清液、尿液等多种类型的样本
8、可检测动物类型是否丰富
9、可检测指标是否齐全
Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法。
但是在选择的时候,也需要注意其他的一些问题。
1、采用何种原料和抗体,是否高效、灵敏、特异
2、规范包被操作,吸附是否均匀
3、重复性、可靠性
6、是否提供技术服务
7、适用于血浆、血清、组织匀浆液、细胞培养上清液、尿液等多种类型的样本
8、可检测动物类型是否丰富
9、可检测指标是否齐全
elisa试剂盒 就查下博欧特生物
使用方法:
1、 血清:操作过程中避免任何细胞刺激。使用不含热原和内毒素的试管。收集血液后,1000×g离心10分钟将血红细胞迅速小心地分离。
2、 血浆:EDTA、柠檬酸盐、肝素血浆可用于检测。1000×g离心30分钟去除颗粒。
3、 细胞上清液:1000×g离心10分钟去除颗粒和聚合物。
4、 组织匀浆:将组织加入适量生理盐水捣碎。1000×g离心10分钟,取上清液。
5、 保存:如果样品不立即使用,应将其分成小部分-70℃保存,避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒,检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。