High Sensitive ELISA Kit for Interleukin 10 (IL10)
CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor
- Product No.HEA056Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range1.56-100pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.44pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 511 For more details, please contact local distributors!US$ 730 For more details, please contact local distributors!US$ 3285 For more details, please contact local distributors!US$ 6205 For more details, please contact local distributors!US$ 51100 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the High Sensitive ELISA Kit for Interleukin 10 (IL10)
This assay has high sensitivity and excellent specificity for detection of High Sensitive Interleukin 10 (IL10).No significant cross-reactivity or interference between High Sensitive Interleukin 10 (IL10) and analogues was observed.
Recovery of the High Sensitive ELISA Kit for Interleukin 10 (IL10)
Matrices listed below were spiked with certain level of recombinant High Sensitive Interleukin 10 (IL10) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Interleukin 10 (IL10) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 94-101 | 97 |
| EDTA plasma(n=5) | 92-99 | 95 |
| heparin plasma(n=5) | 85-98 | 90 |
Precision of the High Sensitive ELISA Kit for Interleukin 10 (IL10)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Interleukin 10 (IL10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Interleukin 10 (IL10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the High Sensitive ELISA Kit for Interleukin 10 (IL10)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Interleukin 10 (IL10) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-97% | 97-105% | 90-104% | 87-94% |
| EDTA plasma(n=5) | 90-104% | 84-99% | 94-102% | 86-102% |
| heparin plasma(n=5) | 93-101% | 86-101% | 79-91% | 97-104% |
Stability of the High Sensitive ELISA Kit for Interleukin 10 (IL10)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the High Sensitive ELISA Kit for Interleukin 10 (IL10)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the High Sensitive ELISA Kit for Interleukin 10 (IL10)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Interleukin 10 (IL10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Interleukin 10 (IL10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Interleukin 10 (IL10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Interleukin 10 (IL10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| EPA056Mu61 | Eukaryotic Interleukin 10 (IL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA056Mu01 | Recombinant Interleukin 10 (IL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA056Mu02 | Recombinant Interleukin 10 (IL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA056Mu61 | Active Interleukin 10 (IL10) | Cell culture; Activity Assays. |
| PAA056Mu01 | Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| PAA056Mu02 | Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| LAA056Mu71 | Biotin-Linked Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC. |
| LAA056Mu81 | FITC-Linked Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IF. |
| MAA056Mu21 | Monoclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| HEA056Mu | High Sensitive ELISA Kit for Interleukin 10 (IL10) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA056Mu | ELISA Kit for Interleukin 10 (IL10) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA056Mu | Mini Samples ELISA Kit for Interleukin 10 (IL10) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA056Mu | CLIA Kit for Interleukin 10 (IL10) | Chemiluminescent immunoassay for Antigen Detection. |
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1、是不是获得SCI文献引用；
2、试剂盒是不是具备高灵敏，质量好的；
3、售后服务完全，提供代测服务；
4、后期实验提供全程技术支持！
今天文章就简单整理了一下最近遇到的问题，有些问题对你来说可能很简单，但是还是会给一些科研菜鸟带去小麻烦，特整理了这么个专场来涨涨姿势。
这里就简单整理了5个问题，具体内容如下：
小编自己在各大论坛看到最多的三个答案：
1）48T可做42个样本加6个标准曲线；96T可做90个样本加6个标准曲线
2）以96T试剂盒来算，定量的试剂盒一般可以做90个左右的样品，定性的试剂盒可以做94个左右的样品
3）96T的ELISA试剂盒，去除标准品复孔检测，最多还能检测80个标本；48T的最多30个样本。
2、elisa试剂盒96T的能做检测多少血清样品？板能重复利用吗？
96T，一般做复孔的话，做标准曲线需要16个孔，剩下80个孔就可以做40个样本。这个板不能重复利用。而且配套的试剂也只是够一块板子用的。
3、想请教一下，我想用elisa法测血清里的一种抗原，查了资料，发现没有现成的试剂盒用，现在就有2种备选方案，一种是买抗体对自己包被（查了R&D等大公司都没有可用于elisa的单抗）。哪种比较好呢？自己包被的会不会比国产的试剂盒可靠呢？
如果你觉得技术不错有队其他公司不放心，可以自己购买抗体对自己包被，这个就是麻烦点，自己包被用着放心，舒心；
另外国内还是有一些资质不错的公司，如北京达科为，上海巧伊，依科赛，深圳欣博盛等等
4、western和ELISA处理样本有何差别？
WesternBlot样品制备要加蛋白裂解液（含有蛋白酶抑制剂），然后冰上研磨，收集于EP管，冰上裂解30min，4度12000rpm离心15min，取上清分装，即得到蛋白样品。建议直接电泳，多余的-80保存。建议分装的蛋白每支略多于一次上样量，一次性用完一支，避免反复冻融。
ELISA样品，直接脑组织冰上匀浆，收集于EP管，同上一样离心，大约220-250ul每支分装（因为ELISA上样量基本都是每孔100ul，做复孔，每个样品2孔，就需要200ul，为了避免不够2孔，分装时要大于200ul每支），直接实验。多余的-80保存，一样避免反复冻融。
不过ELISA最好少量样品进行预实验，或者查阅文献，看看大概组织里有多高浓度，选择合适的试剂盒，测量范围要与组织浓度相符。避免浓度过高测量不准，或者浓度过低，小于试剂盒最低可测浓度。
5、Elisa试剂盒该买哪家公司的呢？
提出这个问题的朋友其实该打，目前国际上各个品牌的试剂盒生厂商，都会有其偏重点，像R&D在细胞因子方面的试剂盒就非常牛逼，而其他牌子也一样的。
Bender：粘附分子方面的试剂盒；
Cayman：花生四烯酸类产品的试剂盒；
Merck(Linco)：内分泌研究方面的试剂盒；
Tips：
1、买Elisa试剂盒时，尽量先查文献，通过文献找品牌，再找供应商，找供应商就找正规代理商，千万不要去干“赔夫人又折兵”的蠢事。
2、ELISA中，复孔就是同一个样品加到至少两个孔中（一般3个或4个），最后取其平均值作为样品的读数，这样测得的结果才有说服力！
做复孔是为了较好的较少组内差异，如果血清不足不做复孔也可以。
