High Sensitive ELISA Kit for Interleukin 10 (IL10)
CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor
- Product No.HEA056Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range1.56-100pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.44pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 511 For more details, please contact local distributors!US$ 730 For more details, please contact local distributors!US$ 3285 For more details, please contact local distributors!US$ 6205 For more details, please contact local distributors!US$ 51100 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the High Sensitive ELISA Kit for Interleukin 10 (IL10)
This assay has high sensitivity and excellent specificity for detection of High Sensitive Interleukin 10 (IL10).No significant cross-reactivity or interference between High Sensitive Interleukin 10 (IL10) and analogues was observed.
Recovery of the High Sensitive ELISA Kit for Interleukin 10 (IL10)
Matrices listed below were spiked with certain level of recombinant High Sensitive Interleukin 10 (IL10) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Interleukin 10 (IL10) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 94-101 | 97 |
| EDTA plasma(n=5) | 92-99 | 95 |
| heparin plasma(n=5) | 85-98 | 90 |
Precision of the High Sensitive ELISA Kit for Interleukin 10 (IL10)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Interleukin 10 (IL10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Interleukin 10 (IL10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the High Sensitive ELISA Kit for Interleukin 10 (IL10)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Interleukin 10 (IL10) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-97% | 97-105% | 90-104% | 87-94% |
| EDTA plasma(n=5) | 90-104% | 84-99% | 94-102% | 86-102% |
| heparin plasma(n=5) | 93-101% | 86-101% | 79-91% | 97-104% |
Stability of the High Sensitive ELISA Kit for Interleukin 10 (IL10)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the High Sensitive ELISA Kit for Interleukin 10 (IL10)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the High Sensitive ELISA Kit for Interleukin 10 (IL10)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Interleukin 10 (IL10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Interleukin 10 (IL10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Interleukin 10 (IL10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Interleukin 10 (IL10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| EPA056Mu61 | Eukaryotic Interleukin 10 (IL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA056Mu01 | Recombinant Interleukin 10 (IL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA056Mu02 | Recombinant Interleukin 10 (IL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA056Mu61 | Active Interleukin 10 (IL10) | Cell culture; Activity Assays. |
| PAA056Mu01 | Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| PAA056Mu02 | Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| LAA056Mu71 | Biotin-Linked Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC. |
| LAA056Mu81 | FITC-Linked Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IF. |
| MAA056Mu21 | Monoclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| HEA056Mu | High Sensitive ELISA Kit for Interleukin 10 (IL10) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA056Mu | ELISA Kit for Interleukin 10 (IL10) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA056Mu | Mini Samples ELISA Kit for Interleukin 10 (IL10) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA056Mu | CLIA Kit for Interleukin 10 (IL10) | Chemiluminescent immunoassay for Antigen Detection. |
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1、采用何种原料和抗体,是否高效、灵敏、特异
2、规范包被操作,吸附是否均匀
3、重复性、可靠性
6、是否提供技术服务
7、适用于血浆、血清、组织匀浆液、细胞培养上清液、尿液等多种类型的样本
8、可检测动物类型是否丰富
9、可检测指标是否齐全
的试剂：免疫吸附剂、结合物和酶的底物。
完整的ELISA试剂盒包含以下各组分：
（1）已包被抗原或抗体的固相载体（免疫吸附剂）；
（2）酶标记的抗原或抗体（结合物）；
（3）酶的底物；
（4）阴性对照品和阳性对照品（定性测定中），参考标准品和控制血清（定量测定
中）；
（5）结合物及标本的稀释液；
（6）洗涤液；
（7）酶反应终止液。
