Instant ELISA Kit for Interferon Alpha (IFNa)
IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D
- Product No.IEA033Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length1h, 10min
- Detection Rangen/a
- Sensitivityn/a
- Sample Typen/a
- Downloadn/a
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 515 For more details, please contact local distributors!US$ 735 For more details, please contact local distributors!US$ 3308 For more details, please contact local distributors!US$ 6248 For more details, please contact local distributors!US$ 51450 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the Instant ELISA Kit for Interferon Alpha (IFNa)
This assay has high sensitivity and excellent specificity for detection of Instant Interferon Alpha (IFNa).No significant cross-reactivity or interference between Instant Interferon Alpha (IFNa) and analogues was observed.
Recovery of the Instant ELISA Kit for Interferon Alpha (IFNa)
Matrices listed below were spiked with certain level of recombinant Instant Interferon Alpha (IFNa) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Interferon Alpha (IFNa) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 88-95 | 91 |
| EDTA plasma(n=5) | 91-101 | 96 |
| heparin plasma(n=5) | 95-103 | 99 |
Precision of the Instant ELISA Kit for Interferon Alpha (IFNa)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Interferon Alpha (IFNa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Interferon Alpha (IFNa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the Instant ELISA Kit for Interferon Alpha (IFNa)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Interferon Alpha (IFNa) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-101% | 93-101% | 86-105% | 96-105% |
| EDTA plasma(n=5) | 99-105% | 78-90% | 80-98% | 78-102% |
| heparin plasma(n=5) | 80-92% | 97-105% | 84-101% | 84-98% |
Stability of the Instant ELISA Kit for Interferon Alpha (IFNa)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the Instant ELISA Kit for Interferon Alpha (IFNa)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 30 minutes at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 30 minutes at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the Instant ELISA Kit for Interferon Alpha (IFNa)
The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Interferon Alpha (IFNa). Standards or samples and HRP-labeled detection antibody specific to Instant Interferon Alpha (IFNa) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Interferon Alpha (IFNa), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Interferon Alpha (IFNa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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1、采用何种原料和抗体,是否高效、灵敏、特异
2、规范包被操作,吸附是否均匀
3、重复性、可靠性
6、是否提供技术服务
7、适用于血浆、血清、组织匀浆液、细胞培养上清液、尿液等多种类型的样本
8、可检测动物类型是否丰富
9、可检测指标是否齐全
的试剂：免疫吸附剂、结合物和酶的底物。
完整的ELISA试剂盒包含以下各组分：
（1）已包被抗原或抗体的固相载体（免疫吸附剂）；
（2）酶标记的抗原或抗体（结合物）；
（3）酶的底物；
（4）阴性对照品和阳性对照品（定性测定中），参考标准品和控制血清（定量测定
中）；
（5）结合物及标本的稀释液；
（6）洗涤液；
（7）酶反应终止液。
