Mini Samples ELISA Kit for Interleukin 2 (IL2)
TCGF; Lymphokine; T-Cell Growth Factor; Aldesleukin
- Product No.MEA073Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.0pg/mL.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 375 For more details, please contact local distributors!US$ 535 For more details, please contact local distributors!US$ 2408 For more details, please contact local distributors!US$ 4548 For more details, please contact local distributors!US$ 37450 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the Mini Samples ELISA Kit for Interleukin 2 (IL2)
This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 2 (IL2).No significant cross-reactivity or interference between Mini Samples Interleukin 2 (IL2) and analogues was observed.
Recovery of the Mini Samples ELISA Kit for Interleukin 2 (IL2)
Matrices listed below were spiked with certain level of recombinant Mini Samples Interleukin 2 (IL2) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Interleukin 2 (IL2) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 83-105 | 91 |
| EDTA plasma(n=5) | 82-94 | 89 |
| heparin plasma(n=5) | 78-93 | 87 |
Precision of the Mini Samples ELISA Kit for Interleukin 2 (IL2)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 2 (IL2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 2 (IL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the Mini Samples ELISA Kit for Interleukin 2 (IL2)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Interleukin 2 (IL2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-98% | 86-102% | 89-98% | 90-97% |
| EDTA plasma(n=5) | 82-94% | 85-96% | 92-105% | 99-105% |
| heparin plasma(n=5) | 80-101% | 95-103% | 87-101% | 93-103% |
Stability of the Mini Samples ELISA Kit for Interleukin 2 (IL2)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the Mini Samples ELISA Kit for Interleukin 2 (IL2)
1. Prepare all reagents, samples and standards;2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 20µL Stop Solution. Read at 450nm immediately.
Test principle of the Mini Samples ELISA Kit for Interleukin 2 (IL2)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Interleukin 2 (IL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Interleukin 2 (IL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Interleukin 2 (IL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Interleukin 2 (IL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| EPA073Mu61 | Eukaryotic Interleukin 2 (IL2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA073Mu01 | Active Interleukin 2 (IL2) | Cell culture; Activity Assays. |
| RPA073Mu02 | Recombinant Interleukin 2 (IL2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA073Mu01 | Recombinant Interleukin 2 (IL2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA073Mu61 | Active Interleukin 2 (IL2) | Cell culture; Activity Assays. |
| PAA073Mu02 | Polyclonal Antibody to Interleukin 2 (IL2) | WB; IHC; ICC; IP. |
| PAA073Mu01 | Polyclonal Antibody to Interleukin 2 (IL2) | WB; IHC; ICC; IP. |
| MAA073Mu21 | Monoclonal Antibody to Interleukin 2 (IL2) | WB; IHC; ICC; IP. |
| MEA073Mu | Mini Samples ELISA Kit for Interleukin 2 (IL2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA073Mu | ELISA Kit for Interleukin 2 (IL2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
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| KSA073Mu01 | ELISA Kit DIY Materials for Interleukin 2 (IL2) | Main materials for "Do It (ELISA Kit) Yourself". |
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的试剂：免疫吸附剂、结合物和酶的底物。
完整的ELISA试剂盒包含以下各组分：
（1）已包被抗原或抗体的固相载体（免疫吸附剂）；
（2）酶标记的抗原或抗体（结合物）；
（3）酶的底物；
（4）阴性对照品和阳性对照品（定性测定中），参考标准品和控制血清（定量测定
中）；
（5）结合物及标本的稀释液；
（6）洗涤液；
（7）酶反应终止液。
elisa试剂盒回收率是反应待测物在样品分析过程中的损失的程度，损失越少，回收率越高，如果作标液1PPM，就是1毫克/升，而作出标准数据为0.99毫克/升，就是说你的回收率是99%，这个与真实成分有密切的关系，说明方法的准确度。比如水中总无机氯含量测定，样品水中含有无机氯20mg/L，取100mL被测水样品，加入0.1mL浓度为10mg/mL的含无机氯标准样品，测定时忽略体积变化，如果测定出样品中无机氯为2.98mg/L，则认为回收率为99%。向左转|向右转
2、规范包被操作，吸附是否均匀
3、重复性、可靠性
6、是否提供技术
7、适用于血浆、血清、组织匀浆液、细胞培养上清液、尿液等多种类型的样本
8、可检测动物类型是否丰富
9、可检测指标是否齐全
