CLIA Kit for Ciliary Neurotrophic Factor (CNTF)
HCNTF
- Product No.SCA021Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range6.86-5,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.61pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 638 For more details, please contact local distributors!US$ 912 For more details, please contact local distributors!US$ 4104 For more details, please contact local distributors!US$ 7752 For more details, please contact local distributors!US$ 63840 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the CLIA Kit for Ciliary Neurotrophic Factor (CNTF)
This assay has high sensitivity and excellent specificity for detection of Ciliary Neurotrophic Factor (CNTF).No significant cross-reactivity or interference between Ciliary Neurotrophic Factor (CNTF) and analogues was observed.
Recovery of the CLIA Kit for Ciliary Neurotrophic Factor (CNTF)
Matrices listed below were spiked with certain level of recombinant Ciliary Neurotrophic Factor (CNTF) and the recovery rates were calculated by comparing the measured value to the expected amount of Ciliary Neurotrophic Factor (CNTF) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-105 | 101 |
| EDTA plasma(n=5) | 86-94 | 89 |
| heparin plasma(n=5) | 97-105 | 101 |
Precision of the CLIA Kit for Ciliary Neurotrophic Factor (CNTF)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ciliary Neurotrophic Factor (CNTF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ciliary Neurotrophic Factor (CNTF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the CLIA Kit for Ciliary Neurotrophic Factor (CNTF)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ciliary Neurotrophic Factor (CNTF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-96% | 80-91% | 90-99% | 93-101% |
| EDTA plasma(n=5) | 90-101% | 79-89% | 86-98% | 87-95% |
| heparin plasma(n=5) | 78-103% | 80-99% | 96-105% | 96-103% |
Stability of the CLIA Kit for Ciliary Neurotrophic Factor (CNTF)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the CLIA Kit for Ciliary Neurotrophic Factor (CNTF)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;8. Read RLU value immediately.
Test principle of the CLIA Kit for Ciliary Neurotrophic Factor (CNTF)
The microplate provided in this kit has been pre-coated with an antibody specific to Ciliary Neurotrophic Factor (CNTF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ciliary Neurotrophic Factor (CNTF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ciliary Neurotrophic Factor (CNTF) level in the sample or standard.;
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Related products
| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| RPA021Ra01 | Recombinant Ciliary Neurotrophic Factor (CNTF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA021Ra01 | Polyclonal Antibody to Ciliary Neurotrophic Factor (CNTF) | WB; IHC; ICC; IP. |
| LAA021Ra81 | FITC-Linked Polyclonal Antibody to Ciliary Neurotrophic Factor (CNTF) | WB; IHC; ICC; IF. |
| LAA021Ra71 | Biotin-Linked Polyclonal Antibody to Ciliary Neurotrophic Factor (CNTF) | WB; IHC; ICC. |
| MAA021Ra21 | Monoclonal Antibody to Ciliary Neurotrophic Factor (CNTF) | WB; IHC; ICC; IP. |
| SEA021Ra | ELISA Kit for Ciliary Neurotrophic Factor (CNTF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA021Ra | CLIA Kit for Ciliary Neurotrophic Factor (CNTF) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA021Ra01 | ELISA Kit DIY Materials for Ciliary Neurotrophic Factor (CNTF) | Main materials for "Do It(ELISA Kit) Yourself" |
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1、采用何种原料和抗体,是否高效、灵敏、特异
2、规范包被操作,吸附是否均匀
3、重复性、可靠性
6、是否提供技术服务
7、适用于血浆、血清、组织匀浆液、细胞培养上清液、尿液等多种类型的样本
8、可检测动物类型是否丰富
9、可检测指标是否齐全
的试剂：免疫吸附剂、结合物和酶的底物。
完整的ELISA试剂盒包含以下各组分：
（1）已包被抗原或抗体的固相载体（免疫吸附剂）；
（2）酶标记的抗原或抗体（结合物）；
（3）酶的底物；
（4）阴性对照品和阳性对照品（定性测定中），参考标准品和控制血清（定量测定
中）；
（5）结合物及标本的稀释液；
（6）洗涤液；
（7）酶反应终止液。
