
ELISA 2.0 kit for measuring Arachis hypogaea allergen, Ara h 2. | |||||||
Antibodies: | 1C4/pAb | ||||||
Standard Range: | 250-0.5 ng/mL | ||||||
Limit of Detection: | 2.0ng/mL | ||||||
Background: | OD<0.1 at 450nm | ||||||
Coefficient of Determination: | R-squared>0.98 | ||||||
Contents: | Plate: Pre-coated with anti-Ara h 2 monoclonal antibody 1C4Vial 1: (white top) Ara h 2 allergen standardConcentration: 2,500 ng/mlVial 2: (brown) Rabbit anti Ara h 2 antibodyVial 3: (blue top) Peroxidase-conjugated Goat Anti-RabbitBottle 1: Wash buffer, (10x concentrate)Bottle 2: Assay buffer, (10x concentrate)Bottle 3: TMB developing substrateBottle 4: Stop solution (0.5N sulfuric acid) | ||||||
Storage: | The ELISA 2.0 kit should be stored at 2-8°C. | ||||||
Expiry: | 6 months from date of receipt | ||||||
Not Provided: | Type I ultrapure water or 18.2MΩ de-ionized waterVolumetric measuring equipment (e.g. serological pipette, graduated cylinder)Clean containers for buffer and reagent preparationCalibrated single and multi-channel micropipettes and tipsVortex mixerPlate reader capable of reading absorbance at 450nmAnalysis software (recommended, but not required) | ||||||
Notes: | The allergen standard is recommended for immunoassay calibrationpurposes only.A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. | ||||||
Product Resources: | EPC-AH2-1 Certificate of Analysis | ||||||
For research and commercial use in vitro:not for human in vivo or therapeutic use. | |||||||
Certificate of Analysis | |||||||
Pre-coated Plate: | 96-well polystyrene microtiter plate coated withmonoclonal antibody 1C4 and treated with stabilizingagent. Sealed in foil pouch with desiccant. | ||||||
Monoclonal Antibody: | 1C4 | ||||||
Immunogen: | Ara h 2 | ||||||
Isotype: | Mouse IgG1 | ||||||
Specificity: | Binds to a species specific epitope present on Arachis hypogaea allergen, Ara h 2. | ||||||
Purification: | Produced in ascites and purified by affinity chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE. | ||||||
Certificate of Analysis | |||||||
Detection Antibody: | Rabbit polyclonal antiserum | ||||||
Immunogen: | Polyclonal rabbit antiserum raised against natural purified Ara h 2 | ||||||
Isotype | Multiple | ||||||
Specificity: | The antiserum contains IgG antibodies to peanut Arachis hypogaea allergen, Ara h 2. | ||||||
Activity: | Titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free. | ||||||
Certificate of Analysis | |||||||
Allergen Standard: | Purified natural Ara h 2 prepared in 1% BSA/50%glycerol/PBS, pH 7.4. | ||||||
Concentration/Calibration: | 2,500 ng/mL (based on amino acid analysis) | ||||||
References: | |||||||
1. Perry TT, Conover-Walker MK, Pomés A, Chapman MD, Wood RA. Distribution of peanut allergen in the environment. J Allergy Clin Immunol. 2004 May;113(5):973-6. | |||||||
2. Maloney JM, Chapman MD, Sicherer SH. Peanut allergen exposure through saliva: assessment and interventions to reduce exposure. J Allergy Clin Immunol. 2006 Sep;118(3):719-24. | |||||||
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第二步、明确编码该蛋白的基因与其它哪些物种同源性最好
第三步、寻找检测这些物种蛋白的试剂盒
第四步、咨询商家ELISA是试剂盒使用的抗体类型:多抗还是单抗? 单抗是针对什么抗原决定簇的?
第五步、 做出自己的判断该买哪个试剂盒,或者实在没有可以找公司进行订制实验
的试剂:免疫吸附剂、结合物和酶的底物。
完整的ELISA试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定
中);
(5)结合物及标本的稀释液;
(6)洗涤液;
(7)酶反应终止液。
1、是不是获得SCI文献引用;
2、试剂盒是不是具备高灵敏,质量好的;
3、售后服务完全,提供代测服务;
4、后期实验提供全程技术支持!

