The Xfect Protein Transfection Reagent uses a cell-penetrating peptide to bind and transport active proteins directly into a wide variety of mammalian cell types, including hard-to-transfect human suspension cell lines and mouse embryonic stem cells.
The Xfect Protein Transfection Reagent uses a cell-penetrating peptide to bind and transport active proteins directly into a wide variety of mammalian cell types, including hard-to-transfect human suspension cell lines and mouse embryonic stem cells.
What is the Xfect Protein Transfection Reagent and how does it work?
Xfect Protein Transfection Reagent is a modified peptide with cell-penetrating activity whose amino acid composition enables it to interact with a protein cargo and transport this protein across a cell membrane barrier. Just incubate your protein of interest with the Xfect reagent in the supplied buffer for 30 minutes and then apply the mixture to your cells. Two hours later you are ready to assay for protein activity.
Why transfect purified proteins directly?
Protein transfection is extremely rapid compared to traditional gene expression studies using transfected DNA (1–2 hours compared to 18–48 hours) because it bypasses cellular processes such as transcription and translation. It also facilitates studies involving transient effects of proteins, and avoids potentially harmful random DNA integration into the genome of the target cells. Xfect Protein Transfection Reagent makes it possible to deliver active proteins directly into cells for studies that involve transcriptional regulation, the cell cycle, apoptosis, oncogenesis, epigenetics, cell regeneration, and transdifferentiation.
What are the advantages of Xfect Transfection Reagent compared to other protein delivery technologies?
The two technologies most frequently used for protein delivery are based on lipids and cell-penetrating peptides. Lipid-based reagents tend to demonstrate high cytotoxicity, while peptide-based reagents transfect with low efficiency. Xfect Protein Transfection Reagent offers the best advantages of both, i.e., it retains low cytotoxicity and delivers more protein to a higher percentage of target cells (see Image Data tab in the product details section below). Moreover, Xfect Protein Transfection Reagent can transfect cells that are growing at a higher density than competing products, which is important since assays are often performed within a few hours post-transfection and the higher cell densities ensure sufficient material for downstream analysis.
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的试剂:免疫吸附剂、结合物和酶的底物。
完整的ELISA试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定
中);
(5)结合物及标本的稀释液;
(6)洗涤液;
(7)酶反应终止液。
Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法。
但是在选择的时候,也需要注意其他的一些问题。
1、采用何种原料和抗体,是否高效、灵敏、特异
2、规范包被操作,吸附是否均匀
3、重复性、可靠性
6、是否提供技术服务
7、适用于血浆、血清、组织匀浆液、细胞培养上清液、尿液等多种类型的样本
8、可检测动物类型是否丰富
9、可检测指标是否齐全
elisa试剂盒 就查下博欧特生物
使用方法:
1、 血清:操作过程中避免任何细胞刺激。使用不含热原和内毒素的试管。收集血液后,1000×g离心10分钟将血红细胞迅速小心地分离。
2、 血浆:EDTA、柠檬酸盐、肝素血浆可用于检测。1000×g离心30分钟去除颗粒。
3、 细胞上清液:1000×g离心10分钟去除颗粒和聚合物。
4、 组织匀浆:将组织加入适量生理盐水捣碎。1000×g离心10分钟,取上清液。
5、 保存:如果样品不立即使用,应将其分成小部分-70℃保存,避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒,检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。
1、是不是获得SCI文献引用;
2、试剂盒是不是具备高灵敏,质量好的;
3、售后服务完全,提供代测服务;
4、后期实验提供全程技术支持!