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GenWay/in situ Apoptosis Detection Kit/GWB-E868FD/
免费咨询热线
4000-520-616
Description:
| Description | Apoptosisisacellregulationsystemcontrolledbygene.Itisdefinedasaphysiologicalprocessformaintainingindividuallivestocausedeathofcellswhicharedangerousorunnecessary.Apoptosisismuchassociatedwithcellgrowth,differentiation,andotherphysiologicalcellularphenomena,especiallyregardingimmunesystem.Itisnowmanifestthatapoptosisplaysasignificantroleinvariousstagesofcancercellsdevelopment;growth,orproliferationinhibiton,disappearance,differentiationinducedbyphysicalfactors(likerADIation,heat)andbychemicalfactors(ex.medicine).OneofthefeaturesofapoptoticcellsisfragmentationofchromatinDNAatnucleosomelevel(185bp).ThisfragmentatedDNAcanbedetectedhistochemicallybyterminallabeling.TUNEL,anacronymforTerminaldeoxynucleotidyltransferase-mediateddUTP-biotinnickendlabeling,isaeffectivemethodformeasuringtheDNAfragmentsresultingfromtheapoptoticactivationofintracellularendonucleases.FluorochromelabelednucleotidesareinsituincorporatedontotheendsoftheseDNAfragments,allowinghistologiclocalizaionandindividualcellstobedetected. |
|---|---|
| #ofSamples | 20reactions |
| Principleofthetest | TUNELmethodusesTerminaldeoxynucleotidyltransferasetolabel3"-OHendsofDNAfragmentsthataregeneratedduringtheprocessofapoptosis.Thecellsundergoingapoptosisarespecificallylabeledwithfluorescein-dUTPwithhighsensitivity,allowingtheimmediatedetectionbyviewingwithafluorescencemicroscope.Sinceincorporatedfluorescencecanalsobedetectedwithperoxidase-labeledanti-fluorescenceantibody,itispossIBLetodetectwithanlightmicroscope. |
AdditionalInformation:
| Name | insituApoptosisDetectionKit |
|---|---|
| RelatedProductNames | insituapoptosisdetectionKitELISAKitinsituapoptosisdetectionKit |
| IntendedUse | ResearchUseOnly |

