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GenWay/Mouse IgG Antibody (FITC)/GWB-284B18/1 mg
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4000-520-616
AdditionalInformation:
| Name | MouseIgGAntibody(FITC) |
|---|---|
| RelatedProductNames | RABBITF(ab")2ANTIMOUSEIgG:FITCIgGIGHG1 |
| GeneSymbol | Ighg1 |
| GeneFamily | IGH |
| Geneid | 16017 |
| AliasSymbols | IgG1,Igh-4,VH7183,Ighg1 |
| ProteinName | Iggamma-1chainCregion,membrane-boundform |
| DescriptionofTarget | RABBITF(ab")2ANTIMOUSEIgG:FITC |
| SwissprotID | P01869 |
| SpeciesReactivity | Mouse |
| Host | Rabbit |
| Isotype | PolyclonalIgG |
| Conjugation | FITC |
| Replacement | Thisantibodymayreplaceitemsc-52003fromSantaCruzBiotechnology. |
| Immunogen | PurifiedmouseIgG |
| Homology | Mouse |
| ProductFormat | F(ab")2fragmentofIgGconjugatedtoFluoresceinIsothiocyanateIsomerI(FITC)-liquid |
| SwissProtNumber | P01869 |
| Specificity | IgG |
| Concentration | 1mg/ml |
| Applications | F |
| ApplicationInfo | FlowCytometry: 1/200-1/500 |
| Clonality | Polyclonal |
| Format | Solution:Phosphatebufferedsaline,Stabalizer:0.09%,SodiumAzide,Form:F(ab")2fragmentofIgGconjugatedtoFluoresceinIsothiocyanateIsomerI(FITC)-liquid |
| StABIlity | 18monthsfromdateofdespatch. |
| ReconstitutionandStorage | Storeat+4oCorat-20oCifpreferred. Thisproductshouldbestoredundiluted. Storageinfrost-freefreezersisnotrecommended.Thisproductisphotosensitiveandshouldbeprotectedfromlight. Avoidrepeatedfreezingandthawingasthismaydenaturetheantibody.Shouldthisproductcontainaprecipitatewerecommendmicrocentrifugationbeforeuse. |
| Reactivity | Mouse |
| Storage | Storeat+4°Corat-20°Cifpreferred.Thisproductshouldbestoredundiluted.Storageinfrost-freefreezersisnotrecommended.Thisproductisphotosensitiveandshouldbeprotectedfromlight.Avoidrepeatedfreezingandthawingasthism |
| ProtocolInformation | Citation:1:BinzK,JollerP,FroeschP,BinzH,ZapfJ,FroeschER.Repopulationoftheatrophiedthymusindiabeticratsbyinsulin-likegrowthfactorI.ProcNatlAcadSciUSA.1990May;87(10):3690-4.PubMedPMID:2187189;PubMedCentralPMCID:PMC53968. Species:Rat ExperimentName:ImmunofluorescenceandFlowCytometryofthymocytes. ExperimentBackground:FlowcytometryofthymocyteslabeledwithmonoclonalantibodiesrevealedadecreasedexpressionoftheThy-iantigenindiabeticratscomparedwithcontrolrats. ExperimentalSteps:1.Forimmunofluorescencestaining,1x106thymocytessUSPendedin50,ulofPBScontaining0.2%bovineserumalbumin(BSA)wereincubatedat4°Cfor1hrwithasaturatingconcentrationoftheappropriatemAb,washedoncebycentrifugation,incubatedat40Cfor1hrwiththesecondantibody,andwashed.2.Fortwo-colorimmunofluorescence,indirectfluorescenceasdescribedabovewiththemAbOx8wasperformed.3.Thethymocyteswerewashedandthenincubatedwiththedirectlyfluoresceinisothiocyanate(FITC)-labeledmAbW3/25.4.Cellswereanalyzedbyflowcytometryinafluorescenceactivatedcellsystem(FACSanalyzerIFA;BectonDickinson). OtherReagentsUsed:diazepam,streptozotocin,aceticacid,NaCl,glucose NumberOfProtocols:2 |
| LeadTime | Domestic:within2-3weeksdelivery International:2-3weeks |
| IntendedUse | ResearchUseOnly |
| KeyReference | 1.O-Charoenrat,P.etal.(2000)Epidermalgrowthfactor-likeligandsdifferentiallyup-regulatematrixmetalloproteinase9inheadandnecksquamouscarcinomacells.CancerRes.60:1121-1128. 2.Lamote,I.etal.(2004)Influenceof17beta-estrADIol,progesterone,anddexamethasoneondiapedesisandviabilityofbovinebloodpolymorphonuclearleukocytes.J.DairySci.87:3340-3349 |
| :: | PreservativeStabilisers:0.09%-SodiumAzide AntiserumPreparation:AntiseratoMouseIgGwereraisedbyrepeatedimmunisationofrabbitswithhighlypurifiedantigen.PurifiedIgGwaspreparedfromwholeserumbyaffinitychromatography.F(ab")2fragmentswerepreparedbypepsindigestionoftheIgGfollowedbyagelfiltrationsteptoremovetheremainingintactIgGorFcfragments. |
| :: | ApproxProteinConc:F(ab")2concentration1.0mg/ml BufferSolutions:PhosphatebufferedsalinepH7.2 |

