High Sensitive ELISA Kit for Interleukin 6 (IL6)
MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor
- Product No.HEA079Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range1.56-100pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.47pg/mL.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 345 For more details, please contact local distributors!US$ 493 For more details, please contact local distributors!US$ 2219 For more details, please contact local distributors!US$ 4191 For more details, please contact local distributors!US$ 34510 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the High Sensitive ELISA Kit for Interleukin 6 (IL6)
This assay has high sensitivity and excellent specificity for detection of High Sensitive Interleukin 6 (IL6).No significant cross-reactivity or interference between High Sensitive Interleukin 6 (IL6) and analogues was observed.
Recovery of the High Sensitive ELISA Kit for Interleukin 6 (IL6)
Matrices listed below were spiked with certain level of recombinant High Sensitive Interleukin 6 (IL6) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Interleukin 6 (IL6) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 83-99 | 92 |
| EDTA plasma(n=5) | 94-105 | 99 |
| heparin plasma(n=5) | 81-104 | 85 |
Precision of the High Sensitive ELISA Kit for Interleukin 6 (IL6)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Interleukin 6 (IL6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Interleukin 6 (IL6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the High Sensitive ELISA Kit for Interleukin 6 (IL6)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Interleukin 6 (IL6) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-105% | 91-99% | 97-104% | 80-93% |
| EDTA plasma(n=5) | 84-96% | 82-90% | 86-103% | 79-89% |
| heparin plasma(n=5) | 82-89% | 79-95% | 78-90% | 85-99% |
Stability of the High Sensitive ELISA Kit for Interleukin 6 (IL6)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the High Sensitive ELISA Kit for Interleukin 6 (IL6)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the High Sensitive ELISA Kit for Interleukin 6 (IL6)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Interleukin 6 (IL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Interleukin 6 (IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Interleukin 6 (IL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Related products
| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| APA079Ra61 | Active Interleukin 6 (IL6) | Cell culture; Activity Assays. |
| EPA079Ra61 | Eukaryotic Interleukin 6 (IL6) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA079Ra01 | Recombinant Interleukin 6 (IL6) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA079Ra06 | Polyclonal Antibody to Interleukin 6 (IL6) | WB; IHC; ICC; IP. |
| PAA079Ra01 | Polyclonal Antibody to Interleukin 6 (IL6) | WB; IHC; ICC; IP. |
| LAA079Ra71 | Biotin-Linked Polyclonal Antibody to Interleukin 6 (IL6) | WB; IHC; ICC. |
| MAA079Ra21 | Monoclonal Antibody to Interleukin 6 (IL6) | WB; IHC; ICC; IP. |
| MAA079Ra26 | Monoclonal Antibody to Interleukin 6 (IL6) | WB; IHC; ICC; IP. |
| SEA079Ra | ELISA Kit for Interleukin 6 (IL6) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA079Ra | Mini Samples ELISA Kit for Interleukin 6 (IL6) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA079Ra | High Sensitive ELISA Kit for Interleukin 6 (IL6) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA079Ra | CLIA Kit for Interleukin 6 (IL6) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA079Ra01 | ELISA Kit DIY Materials for Interleukin 6 (IL6) | Main materials for "Do It (ELISA Kit) Yourself". |
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现在国内最差也是用3代试剂，有些地方会用4代试剂。
4代试剂（检查抗原+抗体）——窗口期为4周。因为抗原于3-4周达到复制的峰值，此时通过4代试剂检查，如果感染了HIV，抗原/抗体至少有一个为阳性，如果都是阴就排除了。
3代试剂（只查抗体）——窗口期为6周。
以上为理论分析+临床经验的结果，可以说是99.9%的准确度。
但是目前FDA、CDC和试剂生产商统一达成的共识，也就是针对普通人，最保守的窗口期是3个月。无论什么试剂，3个月都100%排除。
复习了很多文献在测定方法里面均提到了CV
例如：
Theintra-assaycoefficientofvariationfortheassaywas4-6%.
Theanalyticcoefficientofvariationwas10.2%
问题是：
我是否可以直接使用说明书里的CV值？
如果不可以，我该如何操作进行计算
DAS-ELISA试剂盒中抗体有包被抗体和酶标抗体，这两种抗体是一种抗体，前者没有酶标，后者用酶进行了标记。
NCM-ELISA一般有一抗和二抗，这两种抗体不一样，前者（一抗）一般是鼠抗体或兔抗体，没有酶标，相对应的二抗一般是羊抗鼠或羊抗兔抗体，用酶进行标记。也有用马大规模备抗体的。
TDS-ELISA是三抗体ELISA，第三级的抗体是将信号级联放大。
以上三种是比较常见的，还有其他一些试剂盒，可参考免疫学的内容。
首先要什么有什么的，你得好好考虑一下。
2、看生产地址
根本没有生产地址，我们知道做实验做产品需要很多的仪器、试剂、耗材，没有人相信一间简单的屋子可以生产各种样的试剂盒。
3、看产品包装
没有任何的生产地址、联系方式等信息，这种产品有问题了连个投诉的地方都没有。
4、看公司网站
有些打着国外原装旗号，整个公司网站为英文页面，实际注册IP地址在中国。如果写着国外的地址，让你国外的朋友实地去看一下！
5、做交叉验证
拿对方提供的几个种类的试剂盒，把里面的关键组份相互替换做做实验，如果交叉严重，只能说明是一种原料生产的试剂盒贴了不同的标签。
6、看价格
价格低得离谱，却打着进口大公司原料分装，核算成本，这种低得离谱的价格是连原料都买不起的。
需要的仪器有：酶标仪、恒温箱、枪、枪头、烧杯、滤纸.当然有自动洗板机最好.
