High Sensitive ELISA Kit for Fibroblast Growth Factor 13 (FGF13)
FHF2; Fibroblast Growth Factor Homologous Factor 2
- Product No.HEC915Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodCompetitive Inhibition
- Assay Length3h
- Detection Range1.23-100pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.53pg/mL.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 539 For more details, please contact local distributors!US$ 770 For more details, please contact local distributors!US$ 3465 For more details, please contact local distributors!US$ 6545 For more details, please contact local distributors!US$ 53900 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the High Sensitive ELISA Kit for Fibroblast Growth Factor 13 (FGF13)
This assay has high sensitivity and excellent specificity for detection of High Sensitive Fibroblast Growth Factor 13 (FGF13).No significant cross-reactivity or interference between High Sensitive Fibroblast Growth Factor 13 (FGF13) and analogues was observed.
Recovery of the High Sensitive ELISA Kit for Fibroblast Growth Factor 13 (FGF13)
Matrices listed below were spiked with certain level of recombinant High Sensitive Fibroblast Growth Factor 13 (FGF13) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Fibroblast Growth Factor 13 (FGF13) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-105 | 85 |
| EDTA plasma(n=5) | 85-97 | 92 |
| heparin plasma(n=5) | 80-94 | 86 |
Precision of the High Sensitive ELISA Kit for Fibroblast Growth Factor 13 (FGF13)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 13 (FGF13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 13 (FGF13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the High Sensitive ELISA Kit for Fibroblast Growth Factor 13 (FGF13)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Fibroblast Growth Factor 13 (FGF13) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-96% | 81-102% | 87-95% | 79-97% |
| EDTA plasma(n=5) | 89-104% | 91-103% | 80-102% | 78-101% |
| heparin plasma(n=5) | 82-95% | 78-101% | 95-105% | 87-99% |
Stability of the High Sensitive ELISA Kit for Fibroblast Growth Factor 13 (FGF13)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the High Sensitive ELISA Kit for Fibroblast Growth Factor 13 (FGF13)
1. Prepare all reagents, samples and standards;2. Add 50µL standard or sample to each well.And then add 50µL prepared Detection Reagent A immediately.Shake and mix. Incubate 1 hour at 37°C;3. Aspirate and wash 3 times;4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;5. Aspirate and wash 5 times;6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle of the High Sensitive ELISA Kit for Fibroblast Growth Factor 13 (FGF13)
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Fibroblast Growth Factor 13 (FGF13) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Fibroblast Growth Factor 13 (FGF13) and unlabeled High Sensitive Fibroblast Growth Factor 13 (FGF13) (Standards or samples) with the pre-coated antibody specific to High Sensitive Fibroblast Growth Factor 13 (FGF13). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Fibroblast Growth Factor 13 (FGF13) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Fibroblast Growth Factor 13 (FGF13) in the sample.
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Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| APC915Hu02 | Active Fibroblast Growth Factor 13 (FGF13) | Cell culture; Activity Assays. |
| RPC915Hu02 | Recombinant Fibroblast Growth Factor 13 (FGF13) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC915Hu01 | Recombinant Fibroblast Growth Factor 13 (FGF13) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC915Hu01 | Active Fibroblast Growth Factor 13 (FGF13) | Cell culture; Activity Assays. |
| CPC915Hu21 | OVA Conjugated Fibroblast Growth Factor 13 (FGF13) | Immunogen; SDS-PAGE; WB. |
| PAC915Hu08 | Polyclonal Antibody to Fibroblast Growth Factor 13 (FGF13) | WB; IHC; ICC; IP. |
| PAC915Hu01 | Polyclonal Antibody to Fibroblast Growth Factor 13 (FGF13) | WB; IHC; ICC; IP. |
| PAC915Hu02 | Polyclonal Antibody to Fibroblast Growth Factor 13 (FGF13) | WB; IHC; ICC; IP. |
| LAC915Hu71 | Biotin-Linked Polyclonal Antibody to Fibroblast Growth Factor 13 (FGF13) | WB; IHC; ICC. |
| LAC915Hu81 | FITC-Linked Polyclonal Antibody to Fibroblast Growth Factor 13 (FGF13) | WB; IHC; ICC; IF. |
| MAC915Hu21 | Monoclonal Antibody to Fibroblast Growth Factor 13 (FGF13) | WB; IHC; ICC; IP. |
| MAC915Hu22 | Monoclonal Antibody to Fibroblast Growth Factor 13 (FGF13) | WB; IHC; ICC; IP. |
| CEC915Hu | ELISA Kit for Fibroblast Growth Factor 13 (FGF13) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEC915Hu | High Sensitive ELISA Kit for Fibroblast Growth Factor 13 (FGF13) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCC915Hu | CLIA Kit for Fibroblast Growth Factor 13 (FGF13) | Chemiluminescent immunoassay for Antigen Detection. |
| CCC915Hu | CLIA Kit for Fibroblast Growth Factor 13 (FGF13) | Chemiluminescent immunoassay for Antigen Detection. |
| KSC915Hu11 | ELISA Kit DIY Materials for Fibroblast Growth Factor 13 (FGF13) | Main materials for "Do It (ELISA Kit) Yourself". |
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首先要什么有什么的，你得好好考虑一下。
2、看生产地址
根本没有生产地址，我们知道做实验做产品需要很多的仪器、试剂、耗材，没有人相信一间简单的屋子可以生产各种样的试剂盒。
3、看产品包装
没有任何的生产地址、联系方式等信息，这种产品有问题了连个投诉的地方都没有。
4、看公司网站
有些打着国外原装旗号，整个公司网站为英文页面，实际注册IP地址在中国。如果写着国外的地址，让你国外的朋友实地去看一下！
5、做交叉验证
拿对方提供的几个种类的试剂盒，把里面的关键组份相互替换做做实验，如果交叉严重，只能说明是一种原料生产的试剂盒贴了不同的标签。
6、看价格
价格低得离谱，却打着进口大公司原料分装，核算成本，这种低得离谱的价格是连原料都买不起的。
现在国内最差也是用3代试剂，有些地方会用4代试剂。
4代试剂（检查抗原+抗体）——窗口期为4周。因为抗原于3-4周达到复制的峰值，此时通过4代试剂检查，如果感染了HIV，抗原/抗体至少有一个为阳性，如果都是阴就排除了。
3代试剂（只查抗体）——窗口期为6周。
以上为理论分析+临床经验的结果，可以说是99.9%的准确度。
但是目前FDA、CDC和试剂生产商统一达成的共识，也就是针对普通人，最保守的窗口期是3个月。无论什么试剂，3个月都100%排除。
复习了很多文献在测定方法里面均提到了CV
例如：
Theintra-assaycoefficientofvariationfortheassaywas4-6%.
Theanalyticcoefficientofvariationwas10.2%
问题是：
我是否可以直接使用说明书里的CV值？
如果不可以，我该如何操作进行计算
DAS-ELISA试剂盒中抗体有包被抗体和酶标抗体，这两种抗体是一种抗体，前者没有酶标，后者用酶进行了标记。
NCM-ELISA一般有一抗和二抗，这两种抗体不一样，前者（一抗）一般是鼠抗体或兔抗体，没有酶标，相对应的二抗一般是羊抗鼠或羊抗兔抗体，用酶进行标记。也有用马大规模备抗体的。
TDS-ELISA是三抗体ELISA，第三级的抗体是将信号级联放大。
以上三种是比较常见的，还有其他一些试剂盒，可参考免疫学的内容。
1．取出试剂盒室温平衡30min，取出血样放至室温。
2．配标准品：取150uL标准品加入150uL标准品稀释液稀释，依次稀释5次。
3．加样：分别于各反应孔中加入标准品50uL，样品40UL，标准品做复孔，样品做3孔。
4．分别于样品孔中加入10UL抗体。
5．标准品和样品孔中分别加入50uL链酶亲和素-HRP，盖上封板膜,轻轻震荡混匀，37℃温育60min。
6．配洗涤液：将30倍浓缩洗涤液用蒸馏水30倍稀释后备用。
7．洗涤：小心揭开封板膜，弃去液体，甩干，每孔加200uL洗涤液，静置30s后弃去，如此重复5次，拍干。
8．显色：每孔先加入显色剂A 50uL，再加显色剂B 50uL，轻轻震荡混匀，37℃避光显色6min。
9． 终止：每孔加入终止液50uL，终止反应（此时蓝色立即转为黄色）。
10．测定：以空白孔调零，450nm波长依序测量各孔的吸光度。测定应在加终止液10min之内进行。
11．保存结果，收拾桌面。
12．分析处理数据
注意事项
1. 取出板条前恢复到室温后再打开外包装袋，实验中不用的板条立即放回包装中，密闭封口，其余不用试剂应盖好。
2. 实验操作中请使用一次性的吸头，避免交叉污染。
3．实验板孔加入试剂的顺序应一致，以保证所有反应孔的孵育时间一致。
4．洗涤过程中反应孔中残留的洗涤液应在滤纸上充分拍干，勿将滤纸直接放入反应孔中吸水。
5．试剂盒内试剂请在保质期内使用，不同批号试剂不要混用。
6．1000pg/ml以上的结果为非线性的，根据此标准曲线无法得到精确的结果。大于1000pg/ml 的样品应以标准稀释缓冲液稀释后重做。在结果分析时，结合考虑相应的稀释度。
