Mini Samples ELISA Kit for Interleukin 4 (IL4)
BSF1; BCGF1 ;B Cell Stimulatory Factor 1; Lymphocyte stimulatory factor 1; B Cell Growth Factor; Binetrakin; Pitrakinra
- Product No.MEA077Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range7.8-500pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.8pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 393 For more details, please contact local distributors!US$ 562 For more details, please contact local distributors!US$ 2529 For more details, please contact local distributors!US$ 4777 For more details, please contact local distributors!US$ 39340 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the Mini Samples ELISA Kit for Interleukin 4 (IL4)
This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 4 (IL4).No significant cross-reactivity or interference between Mini Samples Interleukin 4 (IL4) and analogues was observed.
Recovery of the Mini Samples ELISA Kit for Interleukin 4 (IL4)
Matrices listed below were spiked with certain level of recombinant Mini Samples Interleukin 4 (IL4) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Interleukin 4 (IL4) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-105 | 99 |
| EDTA plasma(n=5) | 98-105 | 102 |
| heparin plasma(n=5) | 85-99 | 96 |
Precision of the Mini Samples ELISA Kit for Interleukin 4 (IL4)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 4 (IL4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 4 (IL4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the Mini Samples ELISA Kit for Interleukin 4 (IL4)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Interleukin 4 (IL4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-95% | 82-93% | 86-93% | 93-105% |
| EDTA plasma(n=5) | 92-101% | 98-105% | 91-105% | 78-96% |
| heparin plasma(n=5) | 92-105% | 94-103% | 94-101% | 85-102% |
Stability of the Mini Samples ELISA Kit for Interleukin 4 (IL4)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the Mini Samples ELISA Kit for Interleukin 4 (IL4)
1. Prepare all reagents, samples and standards;2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 20µL Stop Solution. Read at 450nm immediately.
Test principle of the Mini Samples ELISA Kit for Interleukin 4 (IL4)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Interleukin 4 (IL4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Interleukin 4 (IL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Interleukin 4 (IL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Interleukin 4 (IL4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPA077Mu02 | Recombinant Interleukin 4 (IL4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA077Mu61 | Eukaryotic Interleukin 4 (IL4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA077Mu01 | Recombinant Interleukin 4 (IL4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA077Mu01 | Active Interleukin 4 (IL4) | Cell culture; Activity Assays. |
| APA077Mu61 | Active Interleukin 4 (IL4) | Cell culture; Activity Assays. |
| PAA077Mu06 | Polyclonal Antibody to Interleukin 4 (IL4) | WB; IHC; ICC; IP. |
| PAA077Mu02 | Polyclonal Antibody to Interleukin 4 (IL4) | WB; IHC; ICC; IP. |
| PAA077Mu01 | Polyclonal Antibody to Interleukin 4 (IL4) | WB; IHC; ICC; IP. |
| WEA077Mu | Wide-range ELISA Kit for Interleukin 4 (IL4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA077Mu | Mini Samples ELISA Kit for Interleukin 4 (IL4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA077Mu | ELISA Kit for Interleukin 4 (IL4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA077Mu | CLIA Kit for Interleukin 4 (IL4) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA077Mu01 | ELISA Kit DIY Materials for Interleukin 4 (IL4) | Main materials for "Do It (ELISA Kit) Yourself". |
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复习了很多文献在测定方法里面均提到了CV
例如：
Theintra-assaycoefficientofvariationfortheassaywas4-6%.
Theanalyticcoefficientofvariationwas10.2%
问题是：
我是否可以直接使用说明书里的CV值？
如果不可以，我该如何操作进行计算
需要的仪器有：酶标仪、恒温箱、枪、枪头、烧杯、滤纸.当然有自动洗板机最好.
ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫学活性，酶标记的抗原或抗体既保留其免疫学活性，又保留酶的活性。在测定时，受检标本（测定其中的抗体或抗原）与固相载体表面的抗原或抗体起反应。用洗涤的方法使固相载体上形成的抗原抗体复合物与液体中的其他物质分开。再加入酶标记的抗原或抗体，也通过反应而结合在固相载体上。此时固相上的酶量与标本中受检物质的量呈一定的比例。加入酶反应的底物后，底物被酶催化成为有色产物，产物的量与标本中受检物质的量直接相关，故可根据呈色的深浅进行定性或定量分析。由于酶的催化效率很高，间接地放大了免疫反应的结果，使测定方法达到很高的敏感度。
DAS-ELISA试剂盒中抗体有包被抗体和酶标抗体，这两种抗体是一种抗体，前者没有酶标，后者用酶进行了标记。
NCM-ELISA一般有一抗和二抗，这两种抗体不一样，前者（一抗）一般是鼠抗体或兔抗体，没有酶标，相对应的二抗一般是羊抗鼠或羊抗兔抗体，用酶进行标记。也有用马大规模备抗体的。
TDS-ELISA是三抗体ELISA，第三级的抗体是将信号级联放大。
以上三种是比较常见的，还有其他一些试剂盒，可参考免疫学的内容。
首先要什么有什么的，你得好好考虑一下。
2、看生产地址
根本没有生产地址，我们知道做实验做产品需要很多的仪器、试剂、耗材，没有人相信一间简单的屋子可以生产各种样的试剂盒。
3、看产品包装
没有任何的生产地址、联系方式等信息，这种产品有问题了连个投诉的地方都没有。
4、看公司网站
有些打着国外原装旗号，整个公司网站为英文页面，实际注册IP地址在中国。如果写着国外的地址，让你国外的朋友实地去看一下！
5、做交叉验证
拿对方提供的几个种类的试剂盒，把里面的关键组份相互替换做做实验，如果交叉严重，只能说明是一种原料生产的试剂盒贴了不同的标签。
6、看价格
价格低得离谱，却打着进口大公司原料分装，核算成本，这种低得离谱的价格是连原料都买不起的。
