- The Adeno-X Rapid Titer Kit allows functional, hexon-based adenovirus titration.
- The Adeno-X qPCR Titration Kit allows ultra-fast adenovirus titration using quantitative PCR.
- Each Adeno-X GoStix cassette is a sensitive instant test to detect adenovirus early during amplification and to determine the optimum harvest time.
- The Adeno-X Rapid Titer Kit allows functional, hexon-based adenovirus titration
- The Adeno-X qPCR Titration Kit allows ultra-fast adenovirus titration using quantitative PCR
- Each Adeno-X GoStix cassette is a sensitive instant test to detect adenovirus early during amplification and to determine the optimum harvest time
Adeno-X Rapid Titer Kit
This kit is designed around a hexon-specific antibody, which is used to label infected cells. Hexon protein is encoded by the adenoviral genome and is an essential component of the adenoviral capsid required for adenoviral replication, but its expression depends on the E1 gene product. Thus, E1 trans-complementing cell types such as HEK 293 can be used to measure infectious activity because only infected cells will produce the hexon protein. The rapid titer assay takes 1–2 hours to set up and another 3 hours (two days later) to label, stain, and count infected cells. Each stained cell corresponds to a single infectious unit.
Adeno-X qPCR Titration Kit
The Adeno-X qPCR Titration Kit provides a fast, simple, and accurate method for titrating adenoviral stocks from all Ad5-based adenoviral vectors, including recombinant adenovirus created with our Adeno-X expression systems. The protocol combines qPCR and TB Green chemistry, allowing you to determine the viral genome copy number in adenoviral preparations (i.e., crude lysates or purified stocks) from a calibrated DNA standard curve. The kit delivers results in just 4 hours, and because qPCR titration is so fast, target cells can be infected with accurately titrated virus on the same day the virus is harvested.
Adeno-X GoStix
Adeno-X GoStix will rapidly (~10 min) tell you if you are ready to go with your adenovirus prep and is ideal for determining the best time to harvest your virus following amplification. Simply apply 20µl of culture supernatant to the GoStix cassette, chase it through with four drops of buffer and wait 2–20 min for a band to appear in the window of the cassette. The amount of adenovirus detected in the supernatant correlates strongly with adenovirus production inside the cells. Each test is sensitive enough to detect adenovirus in a small amount of crude supernatant and is also used to ensure maximum yields during the adenovirus amplification process (we employ these same test cassettes to determine the optimum time to harvest virus from infected cells). Just 20µl of supernatant is tested each day following infection of HEK 293 cells. The presence of a strong band in the cassette window ensures that high-titer adenovirus is harvested at the peak time.
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现在国内最差也是用3代试剂，有些地方会用4代试剂。
4代试剂（检查抗原+抗体）——窗口期为4周。因为抗原于3-4周达到复制的峰值，此时通过4代试剂检查，如果感染了HIV，抗原/抗体至少有一个为阳性，如果都是阴就排除了。
3代试剂（只查抗体）——窗口期为6周。
以上为理论分析+临床经验的结果，可以说是99.9%的准确度。
但是目前FDA、CDC和试剂生产商统一达成的共识，也就是针对普通人，最保守的窗口期是3个月。无论什么试剂，3个月都100%排除。
复习了很多文献在测定方法里面均提到了CV
例如：
Theintra-assaycoefficientofvariationfortheassaywas4-6%.
Theanalyticcoefficientofvariationwas10.2%
问题是：
我是否可以直接使用说明书里的CV值？
如果不可以，我该如何操作进行计算
DAS-ELISA试剂盒中抗体有包被抗体和酶标抗体，这两种抗体是一种抗体，前者没有酶标，后者用酶进行了标记。
NCM-ELISA一般有一抗和二抗，这两种抗体不一样，前者（一抗）一般是鼠抗体或兔抗体，没有酶标，相对应的二抗一般是羊抗鼠或羊抗兔抗体，用酶进行标记。也有用马大规模备抗体的。
TDS-ELISA是三抗体ELISA，第三级的抗体是将信号级联放大。
以上三种是比较常见的，还有其他一些试剂盒，可参考免疫学的内容。
首先要什么有什么的，你得好好考虑一下。
2、看生产地址
根本没有生产地址，我们知道做实验做产品需要很多的仪器、试剂、耗材，没有人相信一间简单的屋子可以生产各种样的试剂盒。
3、看产品包装
没有任何的生产地址、联系方式等信息，这种产品有问题了连个投诉的地方都没有。
4、看公司网站
有些打着国外原装旗号，整个公司网站为英文页面，实际注册IP地址在中国。如果写着国外的地址，让你国外的朋友实地去看一下！
5、做交叉验证
拿对方提供的几个种类的试剂盒，把里面的关键组份相互替换做做实验，如果交叉严重，只能说明是一种原料生产的试剂盒贴了不同的标签。
6、看价格
价格低得离谱，却打着进口大公司原料分装，核算成本，这种低得离谱的价格是连原料都买不起的。
需要的仪器有：酶标仪、恒温箱、枪、枪头、烧杯、滤纸.当然有自动洗板机最好.
