
Product Name | SensoLyte ® Anti - alpha - Synuclein (Human) ELISA Kit |
Size | 1 kit |
Catalog # | AS-55550-H |
US$ | $546 |
This kit is optimized to detect human alpha-Synuclein (α-Synuclein) in cell and tissue lysate, and in human body fluids such as cerebrospinal fluid (CSF) and plasma. Wells are pre-coated with anti-α-Synuclein monoclonal antibodies, blocked, and stabilized for long-term storage. The amount of α-Synuclein is quantified using sandwich ELISA: rabbit polyclonal anti-α-Synuclein specific antibodies directly conjugated to horseradish peroxidase (HRP) are used to detect captured α-Synuclein.One Step Convenient Format o Pre-coated and pre-blocked 96-well strip plateo Ready-to-use substrate solution and other assay componentso One step assay (samples and detection antibody are added simultaneously)o 1 hour assay time at room temperature (excluding incubation time)Minimal Sample Size o Requires only 10-20 μl of cell and tissue lysate or body fluidsHigh Sensitivity o Detects 5 pg/ml of α-Synuclein (calculated as three standard deviations from the blank)Broad Dynamic Range o8-500 pg α-Synuclein/ml in the assayed sampleRELATED PRODUCTS: alpha-Synuclein Recombinant Proteins | |
Detailed Information | ![]() ![]() |
Storage | 4°C |
Product Citations | Tanriöver G, et al. (2020) Prominent microglial inclusions in transgenic mouse models of α-synucleinopathy that are distinct from neuronal lesions. Acta neuropathologica communications. 8(1):133. doi: 10.1186/s40478-020-00993-8.Oh SH, et al. (2017). The cleavage effect of mesenchymal stem cell and its derived matrix metalloproteinase-2 on extracellular α-synuclein aggregates in parkinsonian models. Stem cells translational medicine. 2017 6(3):949-61. doi:10.5966/sctm.2016-0111Vivacqua G, et al. (2016). Abnormal Salivary Total and Oligomeric Alpha-Synuclein in Parkinson"s Disease. PLoS One. 11(3):e0151156. doi: 10.1371/journal.pone.0151156.Prusiner, B. S. (2015). Evidence for α-synuclein prions causing multiple system atrophy in humans with parkinsonism pnas 112 doi: 10.1073/pnas.1514475112.Woerman, A. L. (2015). Propagation of prions causing synucleinopathies in cultured cells. PNAS 112 doi: 10.1073/pnas.1513426112.Al-Nimer, MS. et al. (2014). Saliva α-synuclein and a high extinction coefficient protein: A novel approach in assessment biomarkers of Parkinson"s disease. North Am J Med Sci 6, 633. doi: 10.4103/1947-2714.147980.Maia, LF. et al. (2013). Changes in amyloid-β and tau in the cerebrospinal fluid of transgenic mice overexpressing amyloid precursor protein. Sci Transl Med 5, 194re2.Smith, LM. et al. (2012). α-Synuclein and anti-α-synuclein antibodies in Parkinsons disease, atypical Parkinson syndromes, REM sleep behavior disorder, and healthy controls. PloS One 7, e52285. |
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现在国内最差也是用3代试剂,有些地方会用4代试剂。
4代试剂(检查抗原+抗体)——窗口期为4周。因为抗原于3-4周达到复制的峰值,此时通过4代试剂检查,如果感染了HIV,抗原/抗体至少有一个为阳性,如果都是阴就排除了。
3代试剂(只查抗体)——窗口期为6周。
以上为理论分析+临床经验的结果,可以说是99.9%的准确度。
但是目前FDA、CDC和试剂生产商统一达成的共识,也就是针对普通人,最保守的窗口期是3个月。无论什么试剂,3个月都100%排除。
复习了很多文献在测定方法里面均提到了CV
例如:
Theintra-assaycoefficientofvariationfortheassaywas4-6%.
Theanalyticcoefficientofvariationwas10.2%
问题是:
我是否可以直接使用说明书里的CV值?
如果不可以,我该如何操作进行计算
DAS-ELISA试剂盒中抗体有包被抗体和酶标抗体,这两种抗体是一种抗体,前者没有酶标,后者用酶进行了标记。
NCM-ELISA一般有一抗和二抗,这两种抗体不一样,前者(一抗)一般是鼠抗体或兔抗体,没有酶标,相对应的二抗一般是羊抗鼠或羊抗兔抗体,用酶进行标记。也有用马大规模备抗体的。
TDS-ELISA是三抗体ELISA,第三级的抗体是将信号级联放大。
以上三种是比较常见的,还有其他一些试剂盒,可参考免疫学的内容。
首先要什么有什么的,你得好好考虑一下。
2、看生产地址
根本没有生产地址,我们知道做实验做产品需要很多的仪器、试剂、耗材,没有人相信一间简单的屋子可以生产各种样的试剂盒。
3、看产品包装
没有任何的生产地址、联系方式等信息,这种产品有问题了连个投诉的地方都没有。
4、看公司网站
有些打着国外原装旗号,整个公司网站为英文页面,实际注册IP地址在中国。如果写着国外的地址,让你国外的朋友实地去看一下!
5、做交叉验证
拿对方提供的几个种类的试剂盒,把里面的关键组份相互替换做做实验,如果交叉严重,只能说明是一种原料生产的试剂盒贴了不同的标签。
6、看价格
价格低得离谱,却打着进口大公司原料分装,核算成本,这种低得离谱的价格是连原料都买不起的。
需要的仪器有:酶标仪、恒温箱、枪、枪头、烧杯、滤纸.当然有自动洗板机最好.

