
Hemoglobin Antibody (Goat)
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For orders greater than 10 grams, please request a quote.
Product Description | This antibody is obtained by immunization with purified human hemoglobin (Hb) and affinity purification with human Hb on agarose. The antibody could be utilized for detection andquantizationof the human hemoglobin using ELISA, WB gold colloid methods. | |||
Species | Goat | |||
Formulation | 5 mg/mL in PBS or lyophilized powder. | |||
Immunogen | Human Hemoglobin whole protein. | |||
Purification | The antibody was affinity purified with pure human hemoglobin on agarose beads. | |||
Specificity | The antibody recognizes human hemoglobin. It may cross-react with hemoglobin of other mammalian species. | |||
Applications | ELISA (0.1-0.5 µg/mL); | |||
Scientific Description | Hemoglobinis theiron-containingoxygen-transportmetalloproteinin thered blood cells.Hemoglobin concentration measurement is among the most commonly performedblood tests, usually as part of acomplete blood count. Inmedicine,hemoglobinuriais a condition in which thehemoglobinis found in abnormally high concentrations in theurine. The condition is often associated withhemolytic anemia, in whichred blood cells(RBCs) are destroyed, thereby increasing levels of freeplasmahemoglobin. Excess hemoglobin is filtered by thekidneys, which excrete it into the urine, giving urine a purple color. | |||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is one year from date of shipping if stored properly. |
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链霉亲和素是与亲和素有相似生物学特性的一种蛋白质,是streptomyces avidinii菌的分泌物,其分子量及结合生物素的能力与鸡蛋清中的亲和素相似,等电点6.0,非特异性结合远比亲和素低。
生物素容易与蛋白质和核酸类等生物大分子结合,形成的生物素衍生物,不仅保持了大分子物质的原有生物活性,而且比恬度高,具多价性。此外,每个亲和素分子有四个生物素结合部位,可同时以多价形式结合生物素化的大分子衍生物和标记物。因此,BAS具有多级放大作用,使其在应用时可极大地提高检测方法的灵敏度。
特异性
亲和素与生物素间的结合具有极高的亲和力,其反应呈高度专一性。因此,BAS的多层次放大作用在提高灵敏度的同时,并不增加非特异性干扰。而且,BAS结合特性不会因反应试剂的高度稀释而受影响,使其在实际应用中可最大限度地降低反应试剂的非特异作用。
稳定性
亲和素结合生物素的亲和常数可为抗原-抗体反应的百万倍,二者结合形成复合物的解离常数很小,呈不可逆反应性;而且酸、碱、变性剂、蛋白溶解酶以及有机溶剂均不影响其结合。因此,BAS在实际应用中,产物的稳定性高,从而可降低操作误差,提高测定的精确度。
普适性
生物素-结合素系统的多功能性还能提供一套统一的研究方法。例如对于某待测分子,已经得到了对于该分子的生物素标记抗原,那么配合结合亲和素的胶体金可以在电镜下观测,配合结合荧光标记的亲和素可以使用流式细胞仪筛选,配合连接到酶的亲和素可以进行ELISA等免疫组化实验。
细胞培养瓶(板)生长面积容量与细胞数(大约)
96孔板 4~5X10 4 35mm培养皿 1X10 6
48 孔板 1.3X10 5 60mm培养皿 2.6X10 6
24孔板 2.5X10 5 100mm培养皿 7X10 6
12孔板 5X10 5 150mm培养皿 1.8X10 7
6孔板 1.2X10 6
细胞瓶面积 容量 工作体积 细胞数目
612.5cm2 25ml 2ml 5X10 5
25cm2 50ml 5ml 1X10 68
35cm2 75ml 10ml 2X10 6
75 cm2 250ml 15ml X10 6
150cm2 700ml 40ml 1.1X10 7
补充:不同孔板所加培养液的液面都不宜太深,一般在2~3mm范围,结合不同孔的底面积就可算出各培养孔的适宜加液量(参考下表)。若加液量过多会影响气体(氧气)交换,而且在搬动过程中易溢出造成污染。具体所加细胞密度依实验的目的不同灵活掌握。