- chroman 1
- Y-27632
- GSK429286A
- RKI-1447
- Y-27632 dihydrochloride
- GSK269962A
| Fasudil (HA-1077) HClProtein kinase inhibitor |
Sample solution is provided at 25 µL, 10mM.
Quality Control & MSDS
- View current batch:
- Purity = 99.76%
- COA (Certificate Of Analysis)
- HPLC
- NMR (Nuclear Magnetic Resonance)
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure
Related Biological Data
Related Biological Data
| Cell experiment [1]: | |
Cell lines | 5637, UM-UC-3 and SCC-4 cell lines |
Preparation method | The solubility of this compound in DMSO is >16.4 mg/ml. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
Reacting condition | 0-30 μM |
Applications | Lysophosphatidic acid and geranylgeraniol induced an increase of cell proliferation and migration in association with promotion of RhoA activity and upregulation of ROCK expression. Fasudil (HA-1077) could suppress cell proliferation and migration, and also induce apoptosis in a dose-dependent manner. Fasudil (HA-1077) also dramatically suppressed the expression of ROCK-I and ROCK-II, but did not affect RhoA activity. |
| Animal experiment [2]: | |
Animal models | Cbl/Cbl-b deficiency-driven murine model of myeloproliferative disorders |
Dosage form | 100 mg/kg daily by oral gavage |
Application | In the Cbl/Cbl-b deficiency-driven murine model of myeloproliferative disorders, by 2 weeks of treatment, total white cell and monocyte counts were significantly lower in mice treated with fasudil. A trend towards improved survival in fasudil-treated mice that did not reach statistical significance was also observed. Notably, prolonged survival beyond 27 weeks was seen in two fasudil-treated mice, nearly twice the 16-week average life-span in the Cbl/Cbl-b DKO MPD model. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
References: [1] Abe, H., et al., The Rho-kinase inhibitor HA-1077 suppresses proliferation/migration and induces apoptosis of urothelial cancer cells. BMC Cancer, 2014. 14: p. 412. [2] William, B.M., et al., Fasudil, a clinically safe ROCK inhibitor, decreases disease burden in a Cbl/Cbl-b deficiency-driven murine model of myeloproliferative disorders. Hematology, 2015. | |
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| Cas No. | 105628-07-7 | SDF | Download SDF |
| Chemical Name | 5-(1,4-diazepan-1-ylsulfonyl)isoquinoline;hydrochloride | ||
| Canonical SMILES | C1CNCCN(C1)S(=O)(=O)C2=CC=CC3=C2C=CN=C3.Cl | ||
| Formula | C14H17N3O2S.HCl | M.Wt | 327.83 |
| Solubility | ≥16.4mg/mL in DMSO | Storage | Store at -20°C |
| Physical Appearance | A solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
Fasudil (HA-1077) HCl is a selective inhibitor of ROCK with IC50 value of 0.74 μM [1].
Rho-associated protein kinase (ROCK) belongs to the AGC family of serine-threonine kinases and plays a pivotal role in regulating a variety of cellular processes. It has been reported that abnormal expression of ROCK is correlated with numerous diseases and infections [2].
Fasudil (HA-1077) HCl is a potent ROCK inhibitor and has a different structure with the reported ROCK inhibitor Y-27632. When tested with 2 human bladder cancer cell lines (5637 and UM-UC-3), Fasudil (HA-1077) HCl treatment inhibits cell proliferation, decreases cell migration and induced cell apoptosis in a dose dependent manner via blocking Rho/ROCK pathway [3]. In oral squmous cell carcinoma SCC-4 cells, administration of Fasudil (HA-1077) HCl markedly decreases cell motility and inhibits cell migration or invasion in a dose dependent manner (1, 50 and 100 μmol/L [4].
In the Cbl/Cbl-b deficiency-driven murine model of myeloproliferative disorders, oral administration of Fasudil (HA-1077) HCl (100 mg/kg, daily) markedly increases the total white cell and monocyte numbers while prolonged survival time has trend but no statistical difference compared with the control group [2].
References: [1].Wickman, G., C. Lan, and B. Vollrath, Functional roles of the rho/rho kinase pathway and protein kinase C in the regulation of cerebrovascular constriction mediated by hemoglobin: relevance to subarachnoid hemorrhage and vasospasm. Circ Res, 2003. 92(7): p. 809-16.[2].William, B.M., et al., Fasudil, a clinically safe ROCK inhibitor, decreases disease burden in a Cbl/Cbl-b deficiency-driven murine model of myeloproliferative disorders. Hematology, 2015.[3].Abe, H., et al., The Rho-kinase inhibitor HA-1077 suppresses proliferation/migration and induces apoptosis of urothelial cancer cells. BMC Cancer, 2014. 14: p. 412. [4].Moreira Carboni, S.S., et al., HA-1077 inhibits cell migration/invasion of oral squamous cell carcinoma. Anticancer Drugs, 2015.
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特点
零耐药性，零药物残留，调控瘤胃微生物，提高饲料吸收，提高产奶量、降低体细胞、防治隐性乳房炎。
主要成分
抗牛乳房炎特异性IgY、乳酸芽孢杆菌、嗜酸乳杆菌、螯合维生素、半胱胺肽、全卵蛋白肽、活性酵母菌、螯合铁、铜、锌，抗病因子、保健因子、有益菌、生物活性复合酶、生物活性小肽等多种高档原料。
产品性状
本品呈黄褐色粉末，有特别的发酵芳香味。
功能特点
采用“三步多相联合发酵”专利技术生产,经深层发酵，离心、真空低温干燥后，辅以免疫多糖和多种科学配制而成。
1、可彻底分解霉变饲料中的弱极性霉菌毒素，可完全替代预防用抗生素;
2、可明显提高奶牛生产的各个指标，试验证明提高产奶量8-20%。延长产奶高峰15-35天，提高乳脂率0.25-0.4%，同时提高乳蛋白含量0.02-0.05%;
3、对防治奶牛乳房炎有特效，可降低乳房炎发病率60-70%，同时防治奶牛酸奶症效果突出;
4、加快犊牛瘤胃发育，可有效降低腹泻率47%，提高饲料利用率2.5%;
5、生物学稳定性高，耐挤压、耐酸碱(PH3-9)、耐高温(90℃加热30分钟活菌损失不到1%);
6、减少粪便排泄量，可降低牛舍氨臭味，减少。
应用范围：
犊牛、青年牛、高产奶牛、干乳奶牛。
使用方法：
①犊牛本品1000g拌料1000-2000斤，如发生腹泻时本品1000g拌料500斤均匀混饲;
②青年牛和干乳奶牛本品1000g拌料1000-2000斤，均匀混饲;
③高产奶牛本品1000g拌料500-1000斤，均匀混饲，如遇天气突变、转群、噪音等各种应激情况本品1000g拌料500-1000斤添加使用。
望采纳！！
英 [kənˈtɪnju:]美 [kənˈtɪnju]
vi.
持续;逗留;维持原状
vt.
延期;使延伸;使持续;继续说
如题，求购做wb时适用于牛的抗纤连蛋白抗体，分装或者试用装皆可，不求多么高质量，做出来的东西能毕业就行，谢谢~~
有没有知道哪个公司可以买到几种牛病毒的荧光标记的抗体？
我要做细胞的免疫荧光实验。
1．Bluetongue蓝舌病毒
2．BovineAdenovirus牛腺病毒
3．BovineParvovirus牛细小病毒
4．BovineRespiratorySyncytialVirus牛呼吸道融合病毒
5．BovineVirusDiarrhoea牛腹泻病毒
6．Reovirus呼肠孤病毒－3
7．InfectiousBovineRhinotracheitis，IBR传染性牛鼻气管炎病毒
8．BovineParainfluenzaVirusType3，PI3牛副流行性感冒3型病毒
9．RABIes狂犬病毒
能提供一种的也可以，多谢啊～～～～～～～
