CLIA Kit for Amphiregulin (AREG)
AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor
- Product No.SCA006Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range1.37-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.59pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 588 For more details, please contact local distributors!US$ 840 For more details, please contact local distributors!US$ 3780 For more details, please contact local distributors!US$ 7140 For more details, please contact local distributors!US$ 58800 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the CLIA Kit for Amphiregulin (AREG)
This assay has high sensitivity and excellent specificity for detection of Amphiregulin (AREG).No significant cross-reactivity or interference between Amphiregulin (AREG) and analogues was observed.
Recovery of the CLIA Kit for Amphiregulin (AREG)
Matrices listed below were spiked with certain level of recombinant Amphiregulin (AREG) and the recovery rates were calculated by comparing the measured value to the expected amount of Amphiregulin (AREG) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 90-98 | 94 |
| EDTA plasma(n=5) | 79-99 | 88 |
| heparin plasma(n=5) | 98-105 | 102 |
Precision of the CLIA Kit for Amphiregulin (AREG)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amphiregulin (AREG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amphiregulin (AREG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the CLIA Kit for Amphiregulin (AREG)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Amphiregulin (AREG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-101% | 93-104% | 93-101% | 95-103% |
| EDTA plasma(n=5) | 79-90% | 84-103% | 80-91% | 98-105% |
| heparin plasma(n=5) | 89-97% | 85-99% | 94-105% | 97-104% |
Stability of the CLIA Kit for Amphiregulin (AREG)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the CLIA Kit for Amphiregulin (AREG)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;8. Read RLU value immediately.
Test principle of the CLIA Kit for Amphiregulin (AREG)
The microplate provided in this kit has been pre-coated with an antibody specific to Amphiregulin (AREG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Amphiregulin (AREG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Amphiregulin (AREG) level in the sample or standard.;
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ELISA / CLIA Experiment Service
INCREMENT SERVICES
Single-component Reagents of Assay KitLysis Buffer Specific for ELISA / CLIAQuality Control of ELISA / CLIACLIA Kit Customized ServiceDisease Model Customized ServiceSerums Customized ServiceTGFB1 Activation ReagentReal Time PCR Experimental ServiceStreptavidin
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| APA006Hu01 | Active Amphiregulin (AREG) | Cell culture; Activity Assays. |
| RPA006Hu01 | Recombinant Amphiregulin (AREG) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA006Hu01 | Polyclonal Antibody to Amphiregulin (AREG) | WB; IHC; ICC; IP. |
| MAA006Hu22 | Monoclonal Antibody to Amphiregulin (AREG) | WB; IHC; ICC; IP. |
| MAA006Hu24 | Monoclonal Antibody to Amphiregulin (AREG) | WB; IHC; ICC; IP. |
| MAA006Hu26 | Monoclonal Antibody to Amphiregulin (AREG) | WB; IHC; ICC; IP. |
| MAA006Hu21 | Monoclonal Antibody to Amphiregulin (AREG) | WB; IHC; ICC; IP. |
| LAA006Hu76 | Biotin-Linked Monoclonal Antibody to Amphiregulin (AREG) | WB; IHC; ICC. |
| SEA006Hu | ELISA Kit for Amphiregulin (AREG) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA006Hu | CLIA Kit for Amphiregulin (AREG) | Chemiluminescent immunoassay for Antigen Detection. |
| PSA006Hu01 | Antibody Pair for Amphiregulin (AREG) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA006Hu01 | ELISA Kit DIY Materials for Amphiregulin (AREG) | Main materials for "Do It (ELISA Kit) Yourself". |
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有没有知道哪个公司可以买到几种牛病毒的荧光标记的抗体？
我要做细胞的免疫荧光实验。
1．Bluetongue蓝舌病毒
2．BovineAdenovirus牛腺病毒
3．BovineParvovirus牛细小病毒
4．BovineRespiratorySyncytialVirus牛呼吸道融合病毒
5．BovineVirusDiarrhoea牛腹泻病毒
6．Reovirus呼肠孤病毒－3
7．InfectiousBovineRhinotracheitis，IBR传染性牛鼻气管炎病毒
8．BovineParainfluenzaVirusType3，PI3牛副流行性感冒3型病毒
9．RABIes狂犬病毒
能提供一种的也可以，多谢啊～～～～～～～
而国内的只有兔抗牛。
就二抗来说，国内的标记二抗质量也很不错。可以用。
特点
零耐药性，零药物残留，调控瘤胃微生物，提高饲料吸收，提高产奶量、降低体细胞、防治隐性乳房炎。
主要成分
抗牛乳房炎特异性IgY、乳酸芽孢杆菌、嗜酸乳杆菌、螯合维生素、半胱胺肽、全卵蛋白肽、活性酵母菌、螯合铁、铜、锌，抗病因子、保健因子、有益菌、生物活性复合酶、生物活性小肽等多种高档原料。
产品性状
本品呈黄褐色粉末，有特别的发酵芳香味。
功能特点
采用“三步多相联合发酵”专利技术生产,经深层发酵，离心、真空低温干燥后，辅以免疫多糖和多种科学配制而成。
1、可彻底分解霉变饲料中的弱极性霉菌毒素，可完全替代预防用抗生素;
2、可明显提高奶牛生产的各个指标，试验证明提高产奶量8-20%。延长产奶高峰15-35天，提高乳脂率0.25-0.4%，同时提高乳蛋白含量0.02-0.05%;
3、对防治奶牛乳房炎有特效，可降低乳房炎发病率60-70%，同时防治奶牛酸奶症效果突出;
4、加快犊牛瘤胃发育，可有效降低腹泻率47%，提高饲料利用率2.5%;
5、生物学稳定性高，耐挤压、耐酸碱(PH3-9)、耐高温(90℃加热30分钟活菌损失不到1%);
6、减少粪便排泄量，可降低牛舍氨臭味，减少。
应用范围：
犊牛、青年牛、高产奶牛、干乳奶牛。
使用方法：
①犊牛本品1000g拌料1000-2000斤，如发生腹泻时本品1000g拌料500斤均匀混饲;
②青年牛和干乳奶牛本品1000g拌料1000-2000斤，均匀混饲;
③高产奶牛本品1000g拌料500-1000斤，均匀混饲，如遇天气突变、转群、噪音等各种应激情况本品1000g拌料500-1000斤添加使用。
望采纳！！
英 [kənˈtɪnju:]美 [kənˈtɪnju]
vi.
持续;逗留;维持原状
vt.
延期;使延伸;使持续;继续说
