
Product Name | HCV Protease FRET Substrate (RET S1) |
Size | 1 mg |
Catalog # | AS-22991 |
US$ | $595 |
This peptide is a HCV protease substrate incorporating an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm. | |
Detailed Information | ![]() ![]() |
Storage | -20°C desiccated and protected from light |
References | Lin, C. et al. J. Biol. Chem. 10, 1074 (2004); Kakiuchi, N. et al. J. Virol. Methods 80, 77 (1999); Liu, Y. et al. Anal. Biochem. 267, 331 (1999); Taliani, M. et al. Anal. Biochem. 240, 60 (1996). |
Molecular Weight | 1548.6 |
Ac-DE-D(Edans)-EE-Abu-ψ-[COO]-AS-K(Dabcyl)-NH2 | |
Sequence(Three-Letter Code) | Ac - Asp - Glu - Asp(EDANS) - Glu - Glu - Abu - ψ - [COO] - Ala - Ser - Lys(DABCYL) - NH2 |
Product Citations | Beran, R. et al. J Virol 83, 3268 (2009).Lin, T. et al. Antimicrob Agents Chemother 53, 1377 (2009).Pan, K. et al. Antimicrob Agents Chemother 10 (2009).Phan, T. et al. J Virol 83, 8379 (2009).Beran, R. et al. J Biol Chem 283, 29929 (2008).Zhou, Y. et al. Antimicrob Agents Chemother 52, 110 (2008).Zhou, Y. et al. (2007). Phenotypic and Structural Analyses of Hepatitis C Virus NS3 Protease Arg155 Variants SENSITIVITY TO TELAPREVIR (VX-950) AND INTERFERON α. J Biol Chem 282, 22619.Zhou, Y. et al. (2007). PHENOTYPIC CHARACTERIZATION OF VAL36 RESISTANT VARIANTS OF HEPATITIS C VIRUS NS34A SERINE PROTEASE. Antimicrob Agents Chemother doi:10.1128/AAC.00863-07.Perni, RB. et al. Antimicrob Agents Chemother 50, 899 (2006).Ferreon, J. et al.J Biol Chem280,20483 (2005).Lin, C. et al.J Biol Chem279, 17508 (2004). |
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而国内的只有兔抗牛。
就二抗来说,国内的标记二抗质量也很不错。可以用。
有没有知道哪个公司可以买到几种牛病毒的荧光标记的抗体?
我要做细胞的免疫荧光实验。
1.Bluetongue蓝舌病毒
2.BovineAdenovirus牛腺病毒
3.BovineParvovirus牛细小病毒
4.BovineRespiratorySyncytialVirus牛呼吸道融合病毒
5.BovineVirusDiarrhoea牛腹泻病毒
6.Reovirus呼肠孤病毒-3
7.InfectiousBovineRhinotracheitis,IBR传染性牛鼻气管炎病毒
8.BovineParainfluenzaVirusType3,PI3牛副流行性感冒3型病毒
9.RABIes狂犬病毒
能提供一种的也可以,多谢啊~~~~~~~

