

Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
- PeptideCKQGFLSSNGQNHFK,correspondingtoaminoacidresidues58-72ofhumanEMR1(Accession Q14246).Extracellular,N-terminus.
- CellsurfacedetectionofEMR1inliveintactmouseJ774macrophagecells:___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+Anti-EMR1(ADGRE1)(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).CellsurfacedetectionofEMR1inliveintacthumanTHP-1monocyticleukemiacells:___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+Anti-EMR1(ADGRE1)(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).
- 1.Legrand,F.etal.(2014)J.AllergyClin.Immunol.133,1439.
- 2.McKnight,A.J.andGordon,S.(1998)J.Leukoc.Biol.63,271.
- 3.Araç,D.etal.(2012)EMBOJ.31,1364.
Humanepidermalgrowthfactor(EGF)–likemodulecontainingmucin-likehormonereceptor1(EMR1)isasurfacereceptorwithunknownfunctionthatbelongstotheadhesionfamilyoftheGprotein-coupledreceptors(GPCR).ThisreceptorisencodedbytheADGRE1andlikeallGPCRs,containsseventransmembranedomains,anintracellularC-terminusandanextracellularN-terminus.TheNH2domainhassixEGF-likedomains.
AdhesionGPCRssharealongN-terminalextracellularsequencescomprisingmultipledomains,separatedfromthetransmembranesegmentsbyaserine/threonine-richdomain,afeaturereminiscentofmucin-like,single-span,integralmembraneglycoproteinswithadhesiveproperties1,2.
EMR1proteinisahomologofF4/80,amurineproteinthatiswidelybeingusedasaMarkerofmurinemacrophagepopulations1.
HumanEMR1isexclusivelyexpressedonmatureeosinophilsinthebloodandbonemarrow andinnasalpolyps.Thus,EMR1is consideredtobeapotentialtherapeutictargetforthetreatmentofeosinophilicdisorders1,3.
Anti-EMR1(ADGRE1)(extracellular)Antibody (#AER-051)isahighlyspecificantibodydirectedagainstanepitopeofthehumanprotein.Theantibodycanbeusedinwesternblotandlivecellimagingapplications.Itrecognizesanextracellularepitopeandisthusidealfordetectingthereceptorinlivingcells.IthasbeendesignedtorecognizeEMR1fromhuman,mouse,andratsamples.
Anti-EMR1(ADGRE1)(extracellular)–FITCAntibody (#AER-051-F)is directlyconjugatedtofluoresceinisothiocyanate(FITC). The antibodycanbeusedinimmunofluorescentapplicationssuchasdirectlivecellflowcytometry.
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而国内的只有兔抗牛。
就二抗来说,国内的标记二抗质量也很不错。可以用。
有没有知道哪个公司可以买到几种牛病毒的荧光标记的抗体?
我要做细胞的免疫荧光实验。
1.Bluetongue蓝舌病毒
2.BovineAdenovirus牛腺病毒
3.BovineParvovirus牛细小病毒
4.BovineRespiratorySyncytialVirus牛呼吸道融合病毒
5.BovineVirusDiarrhoea牛腹泻病毒
6.Reovirus呼肠孤病毒-3
7.InfectiousBovineRhinotracheitis,IBR传染性牛鼻气管炎病毒
8.BovineParainfluenzaVirusType3,PI3牛副流行性感冒3型病毒
9.RABIes狂犬病毒
能提供一种的也可以,多谢啊~~~~~~~

