什么是酶基因的随机突变,名词解释定义是?_
来自 : 蚂蚁淘
- Wash in distilled water for 2 minutes.
- Counterstain with Methyl Green for 5 minutes.
- Wash in distilled water for 2 minutes.
- Air dry and coverslip.
- Results:
- Nuclei - dark greenCytoplasm - light greenSites of enzyme activity - red
Solutions:
Incubating Medium
- Combine 20 ml of buffer solution, 48 ml of distilled water and 4 ml. of substrate solution.
- Combine 3.2 ml of pararosaniline solution with 3.2 ml of sodium nitrite solution. Mix for 1 minute.
- Add the second solution to the first
- Adjust pH to 5.
Buffer Solution
- Anhydrous sodium acetate - 5.9 g
- Sodium barbiturate - 14.7 g
- Distilled water (boiled) - 500 ml
- Do not adjust the pH of the buffer and store at 4°C.
Substrate Solution
- Naphtol AS-BI phosphatease, sodium salt (Sigma) - 40 mg
- N.N-dimethylformamide - 4 ml
Pararosaniline Solution
- Pararosaniline (C.I.# 42500) - 2 g
- 2N HCl - 50 ml
- Use heat to dissolve, filter when cool and store at 4°C.
Sodium Nitrite Solution
- Sodium Nitrite - 1 gm
- Distilled Water - 25 ml
- Prepare fresh and store at 4°C.
Methyl Green
- Methyl Green (C.I.# 42585) - 1 g
- Phosphate/citrate buffer 0.1M pH 4.0 - 100 ml
Citation:
- Gerrits, P. O. and Smid, L., "Staining Procedures for Tissues Embedded in 2-Hydroxyethyl Methacrylate", Heraeus Kulzer.
免责声明 本文仅代表作者个人观点,与本网无关。其创作性以及文中陈述文字和内容未经本站证实,对本文以及其中全部或者部分内容、文字的真实性、完整性、及时性本站不做任何保证或承诺,请读者仅作参考,并请自行核实相关内容。
版权声明 未经蚂蚁淘授权不得转载、摘编或利用其他方式使用上述作品。已经经本网授权使用作品的,应该授权范围内使用,并注明“来源:蚂蚁淘”。违反上述声明者,本网将追究其相关法律责任。