Avalanche®-in vivo Transfection Reagent is extremely powerful for gene functional studies and RNA interference in vivo. This reagent was made by our proprietary formula whichinteracts with nucleic acids, and move into cells via endocytosis. In addition to efficientlytransporting nucleic acidsinto the cells, the formula is also able to lead to the protection of the nucleic acids from lysosomal degradation andthe efficient release of those nucleic acidsfrom lysosomes to ensure maximum function. Avalanche®-in vivo has been proven to be able to perform the maximum delivery of DNA, shRNA, siRNA, or oligonucleotides into different organs in the body. No significant pro-inflammatory responses have been detected using this reagent. Avalanche®-in vivo can be administered via various routes, such as intravenous, intraperitoneal, intratumoral injection, etc. Avalanche®-in vivo Transfection Reagent is the best choice for delivering nucleic acids in vivo.
References:
- Baghel KS, Tewari BN, Shrivastava R, Malik SA, Lone MU, et al. Macrophages promote matrix protrusive and invasive function of breast cancer cells via MIP-1β dependent upregulation of MYO3A gene in breast cancer cells. Oncoimmunology. 2016 Jul 15;5(7):e1196299. PubMed PMID: 27622050; PubMed Central PMCID: PMC5006911.
- Shimoda H, Doi S, Nakashima A, Sasaki K, Doi T, Masaki T. Inhibition of the H3K4 methyltransferase MLL1/WDR5 complex attenuates renal senescence in ischemia reperfusion mice by reduction of p16INK4a. Kidney Int. 2019 Nov;96(5):1162-1175. doi: 10.1016/j.kint.2019.06.021. Epub 2019 Aug 1. PubMed PMID: 31570196.
Features:
- Efficient delivery of DNA, shRNA, siRNA or oligonucleotides in vivo
- 200 µl reagent for 20 transfections in mouse
- Efficient delivery to the lung, liver, kidney, and spleen, liver, pancreas and certain type of tumor via systemic administration
- Delivery via Multiple modes of administration in many species
- Avalanche®-in vivo/nucleic acid conjugated complexes are stable in serum for 16h
- No detectable inflammatory responses
- Efficient siRNA and plasmid DNA delivery via direct subcutaneous tumor injection (multiple tumor types)
- Reproducible results
- Applicable for plasmid DNA/siRNA co-injection
- Developed and manufactured by EZ Biosystems LLC
EZBiosystems美国EZBiosystems™公司是一家全球性转染试剂和基因表达生产服务提供商，公司的研发宗旨是让生命科学研究变得更简单，快速，容易！该公司主要产品之一为新一代转染试剂Avalanche 系列产品，该产品集合了组合化学，分子生物学和细胞生物学技术。公司致力于开发针对不同细胞类型的专用转染试剂。公司研发团队由多个学科经验丰富的专家组成，包括组合化学、脂类化学、生物物理学、分子生物学和细胞生物学。目前,EZBiosystems™主要提供广谱性基因及siRNA体外转染试剂、体内转染试剂、生物治疗性蛋白表达专用转染试剂以及150余种细胞特异性转染试剂。
EZBiosystems特约代理EZBiosystems品牌名称：EZBiosystemsEZBiosystems品牌logo：EZBiosystems品牌官网：http://ezbiosystems.com/EZBiosystems品牌链接：http://www.annoron.com/brand-342.htmlEZBiosystems品牌介绍：EZ生物系统™是一家全球性的基因转染和基因表达产品和服务提供商。EZBiosystems™isaworldwideprovideroftransfectionandgeneexpressionproducts&services.OneofthemajorfocusesofEZBiosystems™hasbeentheresearch,development,andmanufactureofnext-generationtransfectionreagents----theAvalanche®TransfectionReagentseriesbyapplyingourexpertiseincombinatorialchemistry,molecularBIOLOGy,andcellbiology. EZBiosystems品牌相关产品：品牌货号名称规格EZ BiosystemsEZT-CRPR-1Avalanche®-CRISPR Transfection Reagent0.5ml 1.5mlEZ BiosystemsEZT-OMNI-1Avalanche®-Omni Transfection Reagent0.75ml 1.5ml5*1.5mlEZ BiosystemsEZT-EVDY-1Avalanche®-Everyday Transfection Reagent0.75ml 1.5ml
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短截就是把枝条剪短，主要作用是促使其抽生新梢，增加分枝数目，以保证树势健状和正常结果。短截常用于骨干枝组修剪，结果枝组修剪，和树体局部更新复状。
短截按其长度可分为：
① 中短截：在一年生枝的中部短截，剪后萌发的顶端枝条，长势强，下部枝条长势弱。
② 重短截：剪去一年生枝的2/3。剪后萌发出的枝条较强状，一般用于主侧枝延长头修剪。
③ 重剪：剪去一年生枝的3/4－4/5，剪后萌发出的枝条长势强状，常用于发育枝作延长枝头和徒长果枝，中果枝的修剪。
④极重短截：剪去一年生枝的4/5以上，萌发后的枝条中庸偏状，常用于将发育枝和徒长枝培养结果枝组。
⑤留基部2芽剪：剪后萌发枝条较旺盛，常用于预备枝的修剪。对于幼龄树，树势较旺，以培养良好而牢固的树形结构，提早结果为主要目的，以轻短截，少疏间为主，从始果期到盛果期，主要使桃树多结果，并形成好的树形。
它一般在医院、制药企业使用。
试剂盒使用示例： 试剂盒的产生正是为了使实验人员能够摆脱繁重的试剂配制及优化过程，所以试剂盒中一般配备有相应的使用说明书
病毒液通过pre-filterdisc的时候是不是要制造一个真空环境产生压力差使液体通过？这个时候是用自备的针管还是试剂盒配的5毫升的针管啊？
过表达慢病毒
＞10^8 PFU/ml
干扰慢病毒
＞10^8 PFU/ml
