This kit provides a fast and simple method for determining titers of baculovirus stocks. The kit uses a standard immunological assay to accurately determine baculovirus titers within 48 hr, while plaque and end-point dilution assays typically require 5–10 days. In BacPAK Baculovirus expression systems, infected cells express viral antigens long before plaques are formed. The BacPAK rapid titer assay allows titer determination after a much shorter incubation period. Furthermore, the titers obtained are comparable to those obtained with other methods. This kit is suitable for use with any virus stock with a titer of more than 104 pfu/ml and is compatible with all commonly available (AcMNPV-based) baculovirus expression systems.
This kit provides a fast and simple method for determining titers of baculovirus stocks. The kit uses a standard immunological assay to accurately determine baculovirus titers within 48 hr, while plaque and end-point dilution assays typically require 5–10 days. In BacPAK Baculovirus expression systems, infected cells express viral antigens long before plaques are formed. The BacPAK rapid titer assay allows titer determination after a much shorter incubation period. Furthermore, the titers obtained are comparable to those obtained with other methods. This kit is suitable for use with any virus stock with a titer of more than 104 pfu/ml and is compatible with all commonly available (AcMNPV-based) baculovirus expression systems.
The rapid titer immunoassay uses a primary monoclonal antibody raised against an AcMNPV envelope glycoprotein (gp64) to accurately identify virus-infected cells. A secondary HRP-conjugated antibody enables you to visualize infected cells by light microscopy and determine viral titer. Recently, we have improved the protocol to consistently offer our best ever signal-to-noise ratio for identifying cells.
BacPAK qPCR Titration Kit
This kit provides an extremely fast and simple method for titrating your viral stocks. The kit uses a quick DNA purification step before determining viral genome content using qPCR and green intercalating dye technologies. This titration kit requires only 4 hours and works with any AcMNPV-based baculoviral vector. Using qPCR enables you to perform viral harvest and target cell infection on the same day. You can avoid delays that lead to reduced viral infectivity and infect target cells at a known multiplicity of infection (MOI) for more consistent results.The BacPAK qPCR Titration determines the viral genome copy number in baculoviral preparations from a calibrated DNA standard curve. The procedure is simple: viral DNA and BacPAK control DNA are serially diluted and subjected to qPCR. The DNA copy number of each viral sample is then determined by comparing its Ct value to a standard curve generated by plotting the Ct values of the diluted control samples against their respective copy numbers.With its simplicity, reproducibility, and short processing time, the BacPAK qPCR Titration Kit is ideal for determining baculoviral DNA titers. Accurate and rapid titration provides the means to producing consistent results using high-quality viral supernatants in the shortest possible time.
Why you need to know your titer:
- Confirm the success of your packaging reaction to avoid wasting time with your experiments
- Perform consistent experiments
- Ensure sufficient MOIs such that 100% of your cells are transduced
- Control copy number and hence expression levels
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从1～2个T75培养皿的感染细胞培养液中纯化腺病毒
产品简介
本系列试剂盒可快速，高效地从腺病毒感染的细胞培养液中分离纯化腺病毒，相比于传统CsCl超速离心的腺病毒病毒纯化方式（需要24个小时才能完成纯化），本试剂盒可以在1个小时内完成病毒纯化，操作简单，快速，纯化率高，纯化到的病毒颗粒可直接用于下游实验，如细胞和动物感染。
产品特点
?操作简单，快速，可以在1个小时内完成病毒纯化，不依赖于超速离心操作。
?纯化效率高：小量纯化，可从1~2个T75瓶培养的细胞培养液中纯化病毒颗粒高达1x1012VPs
?每个纯化柱，可以重复利用一次，用于纯化相同种类的腺病毒。
保存条件
纯化柱和脱盐柱保存于4℃，其它组分室温保存。
试剂盒组分
Catalog#
V1160-01
Notes
Preps
10
MiniColumns
5
Canbeusedtwice
(Storeat4°C)
Press-OnCap
5
StoreatRT
DesaltingTube*
1
Canberegenarated
(Storeat4°C)
15mLCollectionTube
10
StoreatRT
10xWashBuffer
30mL
StoreatRT
2xElutionBuffer
30mL
StoreatRT
RegenerationBuffer
30mL
StoreatRT
联系方式：
Biomiga(中国）
电话：0573-82651206
QQ:441931287
联系人：张小姐
病毒液通过pre-filterdisc的时候是不是要制造一个真空环境产生压力差使液体通过？这个时候是用自备的针管还是试剂盒配的5毫升的针管啊？
试剂里面含有的是病毒的抗原，现在基本都是人工合成或提取的，跟病毒完全是两回事
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