- Description
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Description
Details
Description:Mouse monoclonal antibody (mAb) to human hepatitis E virus (HEV) antigen encoded by open reading frame three (ORF3)
Purification:Protein G affinity purified
Product Type:Primary antibody
Target Protein:Human hepatitis E virus (HEV) ORF3 antigen
Immunogen:The immunogen is a highly antigenic polypeptide consisting of at least 30 amino acids encoded by ORF3 region of a Chinese HEV strain.
Fusion Myeloma:Sp2/0-Ag14
Species Reactivity:Human hepatitis E virus, others not tested
Cross-Reactivity:No reactivity with HCV-S, HCV-NS3, HCV-NS4
Host / Isotype:Mouse, IgM Kappa
Formulation:Lyophilized
Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00mg/mL
Storage: Store at -20oC
Research Area:Virology
Background:
Type E hepatitis virus (HEV) is transmitted through fecal-oral route. In developing country with hot climate, HEV outbreak is common with a high mortality in pregnant woman. The virus has a 7.6kb positive sense single strained RNA genome enclosed in a capsid without envelope. There are three open reading frames (ORFs) on HEV genome. ORF1 encodes protease, methyl-transferase, helicase and replicase. ORF2 encodes capsid protein. The function of the protein encoded by ORF3 is undefined.
Application:
ELISA:React with immunogen.
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
Product Specificity | mAb anti-HEV, S-13 |
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Application | EIA |
Size | 0.1 mg |
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试剂里面含有的是病毒的抗原,现在基本都是人工合成或提取的,跟病毒完全是两回事
病毒液通过pre-filterdisc的时候是不是要制造一个真空环境产生压力差使液体通过?这个时候是用自备的针管还是试剂盒配的5毫升的针管啊?
如果PCR产物不是很纯,或者PCR扩增条带比较小,PCR产物前面又有较多引物二聚体时,用胶回收,其余用PCR产物纯化试剂盒。
pcr纯化试剂盒和胶回收试剂盒的区别:
PCR纯化试剂盒:是直接水溶解的PAC产物就可以回收,回收效率高,但是只适合单一条带需要纯化测序的时候使用。
PCR凝胶试剂盒:是在PCR产物是混合物,有多条杂带的情况下,先跑胶将杂带分离,然后在将所要的条带位置的胶切下回收,后者的回收效率低,但是很纯净。
胶回收试剂盒操作步骤:
配制琼脂糖EB凝胶,电泳以分离DNA片段。任何类型或等级的琼脂糖都可以使用。
电泳足够时间后,在紫外灯下小心地把所需的DNA的片段切下来。并尽量去除多余的凝胶。
称取空离心管的重量,切下带目的片段的凝胶装在1.5ml离心管中并称其重量,求出凝胶块的重量,近似地确定其体积。一般情况下,凝胶的密度为1g/ml,于是凝胶的体积与重量的关系可按下面换算:凝胶薄片的重量为0.2g 则其体积为0.2ml;加入等倍凝胶体积的Binding Buffer,把混合物置于55℃~65℃水浴中温浴7min至凝胶完全融化,其间每隔2-3分钟混匀一次;
转移700μl的DNA-琼脂糖溶液到一个HiBindTM DNA柱子,并把柱子装在一个干净的2ml收集管内,室温下,10,000×g离心1min,弃去液体。
将柱子重新套回收集管中,加300μl Binding Buffer至HiBind DNA 柱子中;室温下,10,000×g离心 1分钟,去弃滤出液;这一步相当关键,不要忽略此步。
将柱子重新套回收集管中,加入700μl SPW Wash buffer至HiBind DNA柱子中,室温下,10,000×g离心1分钟,去弃滤出液;注:SPW Wash buffer在使用前必须按瓶子标鉴要求用无水乙醇进行稀释。
将柱子重新套回收集管中,重复加入700μl SPW Wash buffer至HiBind DNA柱子中,室温下,10,000×g离心1分钟,去弃滤出液;
弃去液体,将空柱子重新套回收集管中,10,000×g离心1min以甩干柱基质残余的液体。
这步可以去除柱子基质上残余的乙醇,不要省略此步―――对得到好的DNA产量是十分重要的。
把柱子装在一个干净的1.5ml离心管上,加入30~50μl洗脱液或灭菌水上柱子膜上,10,000×g离心1分钟,离心管中的溶液就是纯化的DNA产物,保存于-20度。