Human CHIP Ubiquitin Ligase Kit - Glow-Fold Substrate Summary
STIP1 Homology and U-box Containing Protein 1 (STUB1), also known as Carboxyl Terminus of Hsp70-interacting Protein (CHIP), is a cytoplasmic protein that functions as a U-box Ubiquitin ligase (E3). It is highly expressed in striated muscle and brain and has been observed at lower levels in other organs including the pancreas, lung, liver, and kidney. STUB1/CHIP is 303 amino acids (aa) in length with a predicted molecular weight of 34.8 kDa. Human STUB1/CHIP shares 97% aa sequence identity with the mouse ortholog but only 67% sequence identity with the rat ortholog. It consists of three 34 aa N-terminal tetratricopeptide repeat (TRP) domains (aa 26-127) that are responsible for protein-protein interactions and a C-terminal U-box domain (aa 226-300) that participates in ubiquitination. A central domain containing charged residues lies between the TRP and U-box domains and is thought to be necessary for TRP-dependent interactions. STUB1/CHIP participates in intracellular protein folding/refolding and degradation. It complexes with several molecular chaperone proteins, including HSP70/HSPA1A, HSC70, and HSP90, and modulates their activity. It also facilitates the ubiquitination of chaperone substrates, including nascent CFTR, phosphorylated Tau, p53, PTEN, Synuclein-alpha, and beta-APP, promoting their degradation.
Specifications
Product Datasheets
Background: CHIP/STUB1
Reagents Provided
Citations for Human CHIP Ubiquitin Ligase Kit - Glow-Fold Substrate
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
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- CHIP E3 ligase mediates proteasomal degradation of the proliferation regulatory protein ALDH1L1 during the transition of NIH3T3 fibroblasts from G0/G1 to S-phaseAuthors: QA Khan, P Pediaditak, Y Malakhau, A Esmaeilnia, Z Ashkavand, V Sereda, NI Krupenko, SA KrupenkoPLoS ONE, 2018;13(7):e0199699.2018
- The E3 Ligase CHIP Mediates p21 Degradation to Maintain RadioresistanceAuthors: K Biswas, S Sarkar, K Du, DL Brautigan, T Abbas, JM LarnerMol. Cancer Res, 2017;0(0):.2017
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短截就是把枝条剪短,主要作用是促使其抽生新梢,增加分枝数目,以保证树势健状和正常结果。短截常用于骨干枝组修剪,结果枝组修剪,和树体局部更新复状。
短截按其长度可分为:
① 中短截:在一年生枝的中部短截,剪后萌发的顶端枝条,长势强,下部枝条长势弱。
② 重短截:剪去一年生枝的2/3。剪后萌发出的枝条较强状,一般用于主侧枝延长头修剪。
③ 重剪:剪去一年生枝的3/4-4/5,剪后萌发出的枝条长势强状,常用于发育枝作延长枝头和徒长果枝,中果枝的修剪。
④极重短截:剪去一年生枝的4/5以上,萌发后的枝条中庸偏状,常用于将发育枝和徒长枝培养结果枝组。
⑤留基部2芽剪:剪后萌发枝条较旺盛,常用于预备枝的修剪。对于幼龄树,树势较旺,以培养良好而牢固的树形结构,提早结果为主要目的,以轻短截,少疏间为主,从始果期到盛果期,主要使桃树多结果,并形成好的树形。
将来可能会出现基于免疫方法的试剂盒,不过因为H7N9是个新病毒,爆发至今不足一月,这么快时间还来不及生产抗体,所以目前的检测试剂盒只能是PCR检测试剂盒。
查到了:
4月7日,上海之江生物科技有限公司官方微博称,该公司成功研制禽流感H7N9(2013)核酸测定试剂盒(荧光PCR法),是针对国内此次H7N9病毒最早研制成功的产品,也是国内目前唯一供应的成品化试剂盒。
它一般在医院、制药企业使用。
试剂盒使用示例: 试剂盒的产生正是为了使实验人员能够摆脱繁重的试剂配制及优化过程,所以试剂盒中一般配备有相应的使用说明书
从1~2个T75培养皿的感染细胞培养液中纯化腺病毒
产品简介
本系列试剂盒可快速,高效地从腺病毒感染的细胞培养液中分离纯化腺病毒,相比于传统CsCl超速离心的腺病毒病毒纯化方式(需要24个小时才能完成纯化),本试剂盒可以在1个小时内完成病毒纯化,操作简单,快速,纯化率高,纯化到的病毒颗粒可直接用于下游实验,如细胞和动物感染。
产品特点
?操作简单,快速,可以在1个小时内完成病毒纯化,不依赖于超速离心操作。
?纯化效率高:小量纯化,可从1~2个T75瓶培养的细胞培养液中纯化病毒颗粒高达1x1012VPs
?每个纯化柱,可以重复利用一次,用于纯化相同种类的腺病毒。
保存条件
纯化柱和脱盐柱保存于4℃,其它组分室温保存。
试剂盒组分
Catalog#
V1160-01
Notes
Preps
10
MiniColumns
5
Canbeusedtwice
(Storeat4°C)
Press-OnCap
5
StoreatRT
DesaltingTube*
1
Canberegenarated
(Storeat4°C)
15mLCollectionTube
10
StoreatRT
10xWashBuffer
30mL
StoreatRT
2xElutionBuffer
30mL
StoreatRT
RegenerationBuffer
30mL
StoreatRT
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