
This kit provides a reagent set to allow simple, rapid detection of enterohemorrhagic E. coli (EHEC) by amplification of the verocytotoxin gene using PCR. It has been designed for screening of seven major serotypes within the O-antigen types (O26, O103, O111, O121, O145, O157, and O165). A multiplex PCR enzyme mix, 2X PCR buffer (with Mg2+ and dNTP), primer mix, and controls are supplied with the kit.
This kit provides a reagent set to allow simple, rapid detection of enterohemorrhagic E. coli (EHEC) by amplification of the verocytotoxin gene using PCR. It has been designed for screening of seven major serotypes within the O-antigen types (O26, O103, O111, O121, O145, O157, and O165). A multiplex PCR enzyme mix, 2X PCR buffer (with Mg2+ and dNTP), primer mix, and controls are supplied with the kit.Additionally, this kit allows for simultaneous detection of verocytotoxin type 1, verocytotoxin type 2, and the eae gene. This product is designed for analysis of DNA extracted from food samples using the hot alkaline extraction method. After running multiplex PCR, samples are subjected to gel electrophoresis, allowing visualization of O antigens, verocytotoxin, eae gene, and internal control bands.Note: Associate Professor Atsushi Iguchi of the Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki contributed to the development of this kit.
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从1~2个T75培养皿的感染细胞培养液中纯化腺病毒
产品简介
本系列试剂盒可快速,高效地从腺病毒感染的细胞培养液中分离纯化腺病毒,相比于传统CsCl超速离心的腺病毒病毒纯化方式(需要24个小时才能完成纯化),本试剂盒可以在1个小时内完成病毒纯化,操作简单,快速,纯化率高,纯化到的病毒颗粒可直接用于下游实验,如细胞和动物感染。
产品特点
?操作简单,快速,可以在1个小时内完成病毒纯化,不依赖于超速离心操作。
?纯化效率高:小量纯化,可从1~2个T75瓶培养的细胞培养液中纯化病毒颗粒高达1x1012VPs
?每个纯化柱,可以重复利用一次,用于纯化相同种类的腺病毒。
保存条件
纯化柱和脱盐柱保存于4℃,其它组分室温保存。
试剂盒组分
Catalog#
V1160-01
Notes
Preps
10
MiniColumns
5
Canbeusedtwice
(Storeat4°C)
Press-OnCap
5
StoreatRT
DesaltingTube*
1
Canberegenarated
(Storeat4°C)
15mLCollectionTube
10
StoreatRT
10xWashBuffer
30mL
StoreatRT
2xElutionBuffer
30mL
StoreatRT
RegenerationBuffer
30mL
StoreatRT
联系方式:
Biomiga(中国)
电话:0573-82651206
QQ:441931287
联系人:张小姐
病毒液通过pre-filterdisc的时候是不是要制造一个真空环境产生压力差使液体通过?这个时候是用自备的针管还是试剂盒配的5毫升的针管啊?
试剂里面含有的是病毒的抗原,现在基本都是人工合成或提取的,跟病毒完全是两回事
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