Liposomes are extensively used to study the interaction of proteins, peptides and other molecules with the surface of a lipid membrane. One of the parameters that affects this interaction is the charge of the liposomal membrane. Liposomes are always made in aqueous environment and they are sized to the desired size in liquid state using various methods such as high-pressure extrusion through nano sized pore track etch membranes. Liposome without water is meaningless. In rare occasions, liposomes are freeze dried and proliposomes are formed in the presence of a lyoprotectant such as trehalose. Using a lyoprotectant is necessary in order to maintain the size of the liposomes after rehydration.
DOTAP is a cationic lipid and the liposomes made from DOTAP are positively charged. The amount of the positive charge depends on the percentage of the DOTAP in the formulation. The products in Lyophosome™-DOTAP-based catalog use DOPC as a matrix lipid and various amounts of cationic DOTAP lipid from 0.5% to 50% with various zeta potentials. For many different types of experiments and especially the studies that involve the interaction of a protein or peptide with the surface of liposomes, charged liposomes (cationic or anionic) are used. Charged liposomes are also used for many different types of blood complement studies. Cationic liposomes are mainly used for delivery of genetic material.
Lyophosome™ product catalog is composed of a large selection of freeze-dried liposomes with various types of lipids and wide range of zeta potentials and different properties. Lyophosome™ products should be used by scientists who understand liposome formulation and have the proper equipment to check the size, separate non-encapsulated drugs and do the proper assays. Freeze-dried liposomes cannot be used blindly.
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从1～2个T75培养皿的感染细胞培养液中纯化腺病毒
产品简介
本系列试剂盒可快速，高效地从腺病毒感染的细胞培养液中分离纯化腺病毒，相比于传统CsCl超速离心的腺病毒病毒纯化方式（需要24个小时才能完成纯化），本试剂盒可以在1个小时内完成病毒纯化，操作简单，快速，纯化率高，纯化到的病毒颗粒可直接用于下游实验，如细胞和动物感染。
产品特点
?操作简单，快速，可以在1个小时内完成病毒纯化，不依赖于超速离心操作。
?纯化效率高：小量纯化，可从1~2个T75瓶培养的细胞培养液中纯化病毒颗粒高达1x1012VPs
?每个纯化柱，可以重复利用一次，用于纯化相同种类的腺病毒。
保存条件
纯化柱和脱盐柱保存于4℃，其它组分室温保存。
试剂盒组分
Catalog#
V1160-01
Notes
Preps
10
MiniColumns
5
Canbeusedtwice
(Storeat4°C)
Press-OnCap
5
StoreatRT
DesaltingTube*
1
Canberegenarated
(Storeat4°C)
15mLCollectionTube
10
StoreatRT
10xWashBuffer
30mL
StoreatRT
2xElutionBuffer
30mL
StoreatRT
RegenerationBuffer
30mL
StoreatRT
联系方式：
Biomiga(中国）
电话：0573-82651206
QQ:441931287
联系人：张小姐
病毒液通过pre-filterdisc的时候是不是要制造一个真空环境产生压力差使液体通过？这个时候是用自备的针管还是试剂盒配的5毫升的针管啊？
试剂里面含有的是病毒的抗原，现在基本都是人工合成或提取的，跟病毒完全是两回事
对虾白斑综合症病毒（WSSV）核酸检测试剂盒（PCR-荧光探针法）
对虾白斑综合症病毒（WSSV）核酸检测试剂盒（恒温荧光法—一管式）
对虾桃拉综合症病毒（TSV）核酸检测试剂盒（恒温荧光法）
对虾桃拉综合症病毒（TSV）核酸检测试剂盒（PCR-荧光探针法）
对虾传染性皮下及造血组织坏死症病毒（IHHNV）核酸检测试剂盒（恒温荧光法）
对虾传染性皮下及造血组织坏死症病毒（IHHNV）核酸检测试剂盒（PCR-荧光探针法）
对虾肝胰腺细小病毒（HPV）核酸检测试剂盒（恒温荧光法）
对虾黄头病毒（YHV）核酸检测试剂盒（恒温荧光法）
对虾黄头病毒（YHV）核酸检测试剂盒（PCR-荧光探针法）
对虾杆状病毒（BP）核酸检测试剂盒（恒温荧光法）
对虾杆状病毒（BP）核酸检测试剂盒（PCR-荧光探针法）
对虾传染性肌肉坏死病毒（IMNV）核酸检测试剂盒（恒温荧光法）
对虾传染性肌肉坏死病毒（IMNV）核酸检测试剂盒（PCR-荧光探针法）
罗氏沼虾诺达病毒（MrNV）核酸检测试剂盒（恒温荧光法）
罗氏沼虾诺达病毒（MrNV）核酸检测试剂盒（PCR-荧光探针法）
急性肝胰腺坏死病（AHPND/EMS）病原核酸检测试剂盒（恒温荧光法）
急性肝胰腺坏死病（AHPND/EMS）病原核酸检测试剂盒（PCR-荧光探针法）
对虾偷死野田村病毒（CMNV）核酸检测试剂盒（恒温扩增法）
对虾偷死野田村病毒（CMNV）核酸检测试剂盒（PCR-荧光探针法）
