
Liposome formulations can be broadly divided into cationic, anionic and neutral subtypes. Charged liposomes are used in many different types of studies such as, blood complement studies, gene transfer studies and other biomedical applications to name a few. Cellsome® liposomes made from anionic PEGylated lipids provide certain benefits.
Anionic liposomes offer a number of attributes, relative to the challenges associated with cationic formulations such as, the administration of cationic liposomes results in higher cytotoxicity levels, both in vitro (Zhong et al., 2009) and in vivo (Balazs and Godbey, 2010). Anionic liposomes demonstrate greater stability in solution (Zhigaltsev et al., 2002, Phillips and Heydari, 1996), lead to lower aggregation when compared with neutrally charged liposomes, and have increased endocytosis when compared with cationic (Bajoria et al., 1997) and neutral liposomes.
To improve liposome stability and enhance their circulation times in the blood, a sterically-stabilized, hydrophilic polymer, polyethylene glycol (PEG), has been shown to be the optimal choice for obtaining sterically-stabilized liposomes. Using an Anionic Liposome with PEG will protect the liposomes from circulating proteins, improving their plasma clearance and enhancing their therapeutic effects.
The four products provided that are made from Anionic PEGylated Lipids provided here consist of either DSPC:Chol:DSPG:DSPE-PEG(2000) (60:30:5:5 molar ratio), DOPC:Chol:DOPG:DOPE-PEG(2000) (60:30:5:5 molar ratio), DSPC:Chol:DSPS:DSPE-PEG(2000) (60:30:5:5 molar ratio), or DOPC:Chol:DOPS:DOPE-PEG(2000) (60:30:5:5 molar ratio).
Lyophosome™ product catalog is composed of a large selection of freeze-dried liposomes with various types of lipids and wide range of zeta potentials and different properties. Lyophosome™ products should be used by scientists who understand liposome formulation and have the proper equipment to check the size, separate non-encapsulated drugs and do the proper assays. Freeze-dried liposomes cannot be used blindly.
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从1~2个T75培养皿的感染细胞培养液中纯化腺病毒
产品简介
本系列试剂盒可快速,高效地从腺病毒感染的细胞培养液中分离纯化腺病毒,相比于传统CsCl超速离心的腺病毒病毒纯化方式(需要24个小时才能完成纯化),本试剂盒可以在1个小时内完成病毒纯化,操作简单,快速,纯化率高,纯化到的病毒颗粒可直接用于下游实验,如细胞和动物感染。
产品特点
?操作简单,快速,可以在1个小时内完成病毒纯化,不依赖于超速离心操作。
?纯化效率高:小量纯化,可从1~2个T75瓶培养的细胞培养液中纯化病毒颗粒高达1x1012VPs
?每个纯化柱,可以重复利用一次,用于纯化相同种类的腺病毒。
保存条件
纯化柱和脱盐柱保存于4℃,其它组分室温保存。
试剂盒组分
Catalog#
V1160-01
Notes
Preps
10
MiniColumns
5
Canbeusedtwice
(Storeat4°C)
Press-OnCap
5
StoreatRT
DesaltingTube*
1
Canberegenarated
(Storeat4°C)
15mLCollectionTube
10
StoreatRT
10xWashBuffer
30mL
StoreatRT
2xElutionBuffer
30mL
StoreatRT
RegenerationBuffer
30mL
StoreatRT
联系方式:
Biomiga(中国)
电话:0573-82651206
QQ:441931287
联系人:张小姐
病毒液通过pre-filterdisc的时候是不是要制造一个真空环境产生压力差使液体通过?这个时候是用自备的针管还是试剂盒配的5毫升的针管啊?
试剂里面含有的是病毒的抗原,现在基本都是人工合成或提取的,跟病毒完全是两回事
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对虾黄头病毒(YHV)核酸检测试剂盒(PCR-荧光探针法)
对虾杆状病毒(BP)核酸检测试剂盒(恒温荧光法)
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罗氏沼虾诺达病毒(MrNV)核酸检测试剂盒(恒温荧光法)
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急性肝胰腺坏死病(AHPND/EMS)病原核酸检测试剂盒(恒温荧光法)
急性肝胰腺坏死病(AHPND/EMS)病原核酸检测试剂盒(PCR-荧光探针法)
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