RiboZol™PlusRNAPurificationKitpurifiesandrecoversallsizesandspeciesofRNA,includinglargeRNAssuchasribosomalRNA(rRNA)ormessengerRNA(mRNA),andsmallRNAslessthan200nucleotidesincludingmicroRNAs(miRNA)andsmallinhibitoryRNAs(siRNA).ThekitcombinesthebenefitsoforganicRNAextractionusingRiboZol™RNAExtractionReagentwiththeeaseandconvenienceofspincolumntechnology.RiboZol™RNAExtractionReagentextractstotalRNAfromcellsortissue,includingdifficultsampletypes.Columnchromatographyemploysaproprietaryresinastheseparationmatrixtoeliminatethetime-consumingisopropanolprecipitationstepofthestandardRiboZol™RNApurificationprocedure.Upto35μgoftotalRNAmaybeconcentratedpercolumn.TheRiboZol™PlusRNAPurificationKitprovidessufficientmaterialfor50totalRNApurificationprocedures.Thekitcontainsthefollowing:
RiboZol™RNAExtractionReagent,2x30ml
RiboZol™WashBuffer,22mls(add50ml95%ethanolforworkingstock)
RiboZol™ElutionBuffer,6ml
RiboZol™SpinColumns,50pieces
RiboZol™CollectionTubes,50pieces
Sufficientmaterialfor50preparations.
