货号：	325-106；325-150
英文名：	Hybrid-RmiRNA
保存条件：	Roomtemperature(15~25℃)/4℃(RiboEx)

Cat.No.	ProductDescription	Size
325-106	Hybrid-RTM miRNA	6
325-150	Hybrid-RTM miRNA	50

 



Description
 
Inrecentyears,interestinsmallRNA,suchassiRNAandmiRNAwhicharerelatedtoresearchofgeneregulation,hasexpanded.TherearemanycommercialkitsfortotalRNApreparation,butmostofthesearefocusedonpreparationoflargeRNAlongerthan200nucleotides.BecausebothsiRNAandmiRNAarebetween15~30nucleotidesinlength,theneedofspeciallyoptimizedkitforsmallRNA(<200nucleotides)isgrowingrapidly.Hybrid-R^TM miRNAisdesignedforpurificationoflargeandsmallRNAseparatelyfromculturecellsoranimaltissuesandco-purificationinasingletubeisalsoavailablebymodifiedprotocol.ThiskitutilizesthelysismethodofRiboEx^TM whichhasapowerfulabilityoflysisandthepurificationmethodbasedonglassfibermembranetechnology.SamplesarehomogenizedinRiboEx^TM,amonophasicsolutioncontainingphenolandguanidiumsalt,whichrapidlylysecellsandinactivatesnucleases.Additionofchloroformbringsaboutaseparationofthelysateintoaqueousandorganicphases.TotalRNAlocatesintheaqueousphasewhileDNAandproteinremainintheinterphaseandorganicphase.LargeandsmallRNAintheaqueousphaseisselectivelyboundtocolumntypeBandtypeWrespectively.ThecolumntypeBselectivelyadsorbstheRNAlargerthan200nucleotidesinlength,whilethecolumntypeWspecificallyholdstheRNAsmallerthan200nucleotidesinlength.TopurifylargeRNA,theaqueousphaseismixedwithethanolandthemixtureisappliedtoacolumntypeB.Aftercentrifugation,largeRNAisboundtomembraneandthemixturecontainingsmallRNAgoesintocollectiontubethroughthemembrane.Themembraneiswashedawaybytwowashbuffer(SW1andRNW)andpurifiedlargeRNAiselutedfromthemembranebyRNase-freewater.TopurifysmallRNA,thepass-throughcomefromthebindingoflargeRNAismixedwithethanolandthenappliedtoacolumntypeW.AfterwashingwithbufferRBWandRNW,smallRNAiselutedbyRNase-freewater.TheprocedureofHybrid-R^TM miRNAtakesonly30minutesforcompletepreparationsofpureRNA.ThepurifiedRNAissuitablefortheisolationofPolyA+RNA,northernblotting,dotblotting,invitrotranslation,cloning,RT-PCR,RPAandotheranalyticalprocedures.
 



FeaturesandBenefits
 
■Preparationtime:~30minutes
■Stableandconsistentyield
■Highpurityandyield
■PerfectseparationofsmallRNAfragment
■Samplesize:Upto50mgtissueorupto1x10⁷ culturedcells
■Recoveryrange:LargeRNA:>200nucleotides
          SmallRNA:<200nucleotides
■Instantuse:Noneedofadditionalmaterials
■Noethanolprecipitation
■NoGenomicDNAcontamination
■Readyforuseinnorthernblotting,dotblotting,invitrotranslation,cloning,RT-PCR,RPAandother analyticalprocedures
 


Procedure