Fetal Bovine Serum, Australia OriginProduct Code : Z7010FBS
zeta-life Fetal Bovine Serum (FBS) promotes steady, healthy cell growth. We can source our FBS cell culture medium supplement from Australia, New Zealand, the US,
as well as USDA approved regions in North America. We provide a Certificate of Analysis for all lots.
Fetal Bovine Serum - FBS
FBS is the most highly used growth factor in cell culture due to its high level of nutrients
Formulation of serum containing and serum reduced media for mammalian cell culture for the production of antibodies, antigens, stem cells and vaccines
Cryopreservation, storage and shipping of biological materials such as cell lines and antibodies
Base matrices for diagnostic kit controls
Blocking agent for immunoassays and blotting
All fetal bovine serum is fully traceable and is sourced from audited suppliers
We process our FBS in single containers and triple 0.1 μ sterile filter it. This eliminates any cross contamination potential.
Custom serum packages available including charcoal treated, heat inactivated, and low endotoxin levels. FBS packaged in 500mL and 1L bottles, or custom containers
that may indicate toxic components in the serum.Total Protein :Determined by Biuret Colorimetry.Protein specification: 30 to 45 g/lCell Culture :Biological performance is assessed using cell culture medium supplemented with the serum beingtested.During the test period, cultures are examined microscopically for any morphological abnormalitiesthat may indicate toxic components in the serum.Cell Culture Tests :Cell Growth, Plating Efficiency, Cloning Efficiency.Cell Lines Tested :The following cell lines are tested with the serum:HELA -Cancer Cell/Human.L929 -Fibroblast-Mouse/ As MacrophageSP2/0-AG14 -Mouse/LymphomaMRC- 5 -Human/Lung.Country of Origin :The country in which the serum was taken from the donor/animal.Storage conditions :Store at -20°CShelf life :5 yearsSterility : Sterile filteredState : FrozenShipping conditions : -20°C or dry ice
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逆转录（reverse transcription）是以RNA为模板合成DNA的过程，即RNA指导下的DNA合成。此过程中，核酸合成与转录（DNA到RNA）过程与遗传信息的流动方向（RNA到DNA）相反，故称为逆转录。逆转录过程是RNA病毒的复制形式之一，需逆转录酶的催化。 逆转录过程的揭示是分子生物学研究中的重大发现，是对中心法则的重要修正和补充。人们通过体外模拟该过程，以样本中提取的mRNA为模板，在逆转录酶的作用下，合成出互补的cDNA，构建cDNA文库，并从中筛选特异的目的基因。该方法已成为基因工程技术中最常用的获得目的基因的策略之一。
1.模板提取（一般为RNA）：Trizol、氯仿、异丙醇、无水乙醇、DEPC处理水
2.模板浓度测定：分光光度计或NanoDrop
3.逆转录：逆转录试剂盒（或者一步法试剂盒），这一步可以用普通PCR做，也可以用水域做。
4.荧光定量PCR试剂：通常有用SYBR Green Mix做的，但是这里建议你用EvaGreen做，灵敏度和平行性都要好于SYBR Green，并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他：除了以上的那些还需要离心管、PCR管或板（Axygen反应比较好）、移液枪等，暂时就想到这么多。
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大家miRNA逆转录和qPCR的试剂盒用的是那个公司的啊，求推荐！
