
Fetal Bovine Serum, Australia OriginProduct Code : Z7010FBS
zeta-life Fetal Bovine Serum (FBS) promotes steady, healthy cell growth. We can source our FBS cell culture medium supplement from Australia, New Zealand, the US,
as well as USDA approved regions in North America. We provide a Certificate of Analysis for all lots.
Fetal Bovine Serum - FBS
FBS is the most highly used growth factor in cell culture due to its high level of nutrients
Formulation of serum containing and serum reduced media for mammalian cell culture for the production of antibodies, antigens, stem cells and vaccines
Cryopreservation, storage and shipping of biological materials such as cell lines and antibodies
Base matrices for diagnostic kit controls
Blocking agent for immunoassays and blotting
All fetal bovine serum is fully traceable and is sourced from audited suppliers
We process our FBS in single containers and triple 0.1 μ sterile filter it. This eliminates any cross contamination potential.
Custom serum packages available including charcoal treated, heat inactivated, and low endotoxin levels. FBS packaged in 500mL and 1L bottles, or custom containers
that may indicate toxic components in the serum.Total Protein :Determined by Biuret Colorimetry.Protein specification: 30 to 45 g/lCell Culture :Biological performance is assessed using cell culture medium supplemented with the serum beingtested.During the test period, cultures are examined microscopically for any morphological abnormalitiesthat may indicate toxic components in the serum.Cell Culture Tests :Cell Growth, Plating Efficiency, Cloning Efficiency.Cell Lines Tested :The following cell lines are tested with the serum:HELA -Cancer Cell/Human.L929 -Fibroblast-Mouse/ As MacrophageSP2/0-AG14 -Mouse/LymphomaMRC- 5 -Human/Lung.Country of Origin :The country in which the serum was taken from the donor/animal.Storage conditions :Store at -20°CShelf life :5 yearsSterility : Sterile filteredState : FrozenShipping conditions : -20°C or dry ice
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2.模板浓度测定:分光光度计或NanoDrop
3.逆转录:逆转录试剂盒(或者一步法试剂盒),这一步可以用普通PCR做,也可以用水域做。
4.荧光定量PCR试剂:通常有用SYBRGreenMix做的,但是这里建议你用EvaGreen做,灵敏度和平行性都要好于SYBRGreen,并且如果你那是ABI或者Stratagene的PCR如果用SYBRGreen还需要加一步Rox很麻烦。
5.其他:除了以上的那些还需要离心管、PCR管或板(Axygen反应比较好)、移液枪等,暂时就想到这么多。
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