- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Type I collagen is the most abundant form of collagen in the human body and is synthesized mainly by fibroblasts, osteoblasts, odontoblasts and chondroblasts.It is located in the extracellular matrix of many tissues of the body including cartilage, bone, tendon, skin and the sclera of the eye.Type I collagen is composed of two pro-α1(I) chains, produced from the COL1A1 gene, and one pro-α2(I) chain, produced from the COL1A2 gene.Mutations in the genes that produce collagen type I are responsible for causing various health conditions including Ehlers-Danlos syndrome, osteogenesis imperfecta, osteoporosis and Caffey disease.
Data/Specifications
Format: IgG fraction, freeze-dried (0.5 ml). Reconstitute with 0.5 mldi H2O and store aliquots at -20°C.
Specificity (% at 1:200 RIA dilution):
Rat Collagen, Type I : 100%
Rat Collagen, Types II, III, V : < 0.1%
Human, mouse, chicken Collagen Type I: < 0.1%
Rat elastin: <0.1%
Literature/Support
Anti-Rat Collagen Type I Antibody Insert (PDF, 283 KB)
References:
Dagher, P. C., Hato, T., Mang, H. E., Plotkin, Z., Richardson, Q. V., Massad, M., ... & Sutton, T. A. (2016). Inhibition of toll-like receptor 4 signaling mitigates microvascular loss but not fibrosis in a model of ischemic acute kidney injury.International journal of molecular sciences,17(5), 647.
Sutton, T. A., Hato, T., Mai, E., Yoshimoto, M., Kuehl, S., Anderson, M., ... & Dagher, P. C. (2013). p53 is renoprotective after ischemic kidney injury by reducing inflammation.Journal of the American Society of Nephrology,24(1), 113-124.
Dagher, P. C., Mai, E. M., Hato, T., Lee, S. Y., Anderson, M. D., Karozos, S. C., ... & Sutton, T. A. (2011). The p53 inhibitor pifithrin-α can stimulate fibrosis in a rat model of ischemic acute kidney injury.American Journal of Physiology-Renal Physiology,302(2), F284-F291.
How To Use
For immunostaining of extra or intracellular components inlight microscopy and the quantitation of Collagen Type I inbiological fluids or in culture supernatants.
Applications
- IFA
- IHC(p)
- (SP) RIA
- ELISA
Working Dilutions
- Indirect IFA (with fluoresceine anti-rabbit IgG conjugate): >1:80 on frozen rat tissues (liver, skin...).
- IHC(p): >1:500 on fixed, paraffin-embedded rat tissues
- ELISA: >1:200 (OD <500)
Related Products
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2.模板浓度测定：分光光度计或NanoDrop
3.逆转录：逆转录试剂盒（或者一步法试剂盒），这一步可以用普通PCR做，也可以用水域做。
4.荧光定量PCR试剂：通常有用SYBRGreenMix做的，但是这里建议你用EvaGreen做，灵敏度和平行性都要好于SYBRGreen，并且如果你那是ABI或者Stratagene的PCR如果用SYBRGreen还需要加一步Rox很麻烦。
5.其他：除了以上的那些还需要离心管、PCR管或板（Axygen反应比较好）、移液枪等，暂时就想到这么多。
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