Hito Luxol Fast Blue - PAS OptimStain™ Kit is designed based on Luxol fast blue and Periodic acid-Schiff (PAS) method. This kit is made in a ready-to-use format and offers high quality, rapid staining (can be finished in one hour) of myelin/myelinated axons on paraffin sections or frozen sections. It has been proven to be extremely reliable and sensitive to demonstrate the morphological details of myelin fibers.Myelin is a substance rich in lipids and proteins that forms a layer, the myelin sheath, around only the axon of a neuron and acts as insulation. It is essential for the proper functioning of the nervous system. Demyelination is the loss of the myelin sheath insulating the nerves. This impairs the conduction of signals in the affected nerves, causing impairment in sensation, movement, and cognition. Currently, no cure exists for demyelinating diseases and myelin repair is an active research field. Hito Luxol Hito Luxol Fast Blue - PAS OptimStain™ Kit allows sensitive localization and visualization of the myelin fibers. PAS counterstain provides color contrasts which show morphological features that are particularly significant in the tissue, thus offers a fast and reliable way to determine the extent of demyelination.Hito Luxol Fast Blue - PAS OptimStain™ Kit has been tested extensively on the brains and spinal cords from several species of animals and it is a simple solution for your research.
Hito Luxol Fast Blue - PAS OptimStain™ Kit Solution-1 is designed to contain Alcohol that is flammable. However, shipping hazardous materials internationally come with the high cost and long waiting time for customs clearance. This PreKit is designed for customers who can purchase absolute Alcohol in their own countries, and then add to the PreKit (Solution-1 Stock Solution) for fast and safe usage of the kit.
| Kit Contents | Standard Kit |
| Solution-1 (LFB Stock Solution) | 10 ml |
| Solution-2 (Li2CO3 Solution) | 250 ml |
| Solution-3 (Periodic Acid Solution) | 125 ml |
| Solution-4 (Modified Schiff"s Solution) | 125 ml |
| Solution-5 | 30 ml |
| Solution-6 | 125 ml |
| Dropper Bottle | 1 |
| Staining Jars | 6 |
| User Manual and MSDS | 1 |
Before using Hito Luxol Fast Blue - PAS OptimStain™ PreKit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit): Cryostat or Microtome and light microscopeParaffin embedding equipment (or paraffin sections)Hito Bouin’s Plus Solution (Cat# HTSH0104)Dry ice, isopentane, O.C.T. compound (for frozen sections), ethanol, xylene, 4% PFA (Hito Cat# HTSHS0102), double distilled or deionized waterSlides and coverslipsStaining jars for slides washResinous mounting medium  Hito Luxol Fast Blue - PAS OptimStain™ PreKit Manual and MSDS | |
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转录的只是插入的片断，怎么会跟模板一样大呢，一般情况下应该比模板小多了。我都是跑电泳，严格来说应该跑变性的；嫌麻烦可以非变性，快速（防止RNA降解）十分钟即可；抛出来的带会有两条，上面大的是模板，下面一条比较亮的是RNA，那么你的转录就 成功了。
PCR第一个步骤如果没记错的话应该叫“变性”， PCR的话是不需要解旋酶的，直接利用DNA的高温变性特性，在高温下，使氢键断开，DNA双链恢复为单链。 （还有解旋酶好像是解除双螺旋的结构，是一种拓扑异构酶，对于氢键的打开没有贡献吧
1.模板提取（一般为RNA）：Trizol、氯仿、异丙醇、无水乙醇、DEPC处理水
2.模板浓度测定：分光光度计或NanoDrop
3.逆转录：逆转录试剂盒（或者一步法试剂盒），这一步可以用普通PCR做，也可以用水域做。
4.荧光定量PCR试剂：通常有用SYBR Green Mix做的，但是这里建议你用EvaGreen做，灵敏度和平行性都要好于SYBR Green，并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他：除了以上的那些还需要离心管、PCR管或板（Axygen反应比较好）、移液枪等，暂时就想到这么多。
逆转录（reverse transcription）是以RNA为模板合成DNA的过程，即RNA指导下的DNA合成。此过程中，核酸合成与转录（DNA到RNA）过程与遗传信息的流动方向（RNA到DNA）相反，故称为逆转录。逆转录过程是RNA病毒的复制形式之一，需逆转录酶的催化。 逆转录过程的揭示是分子生物学研究中的重大发现，是对中心法则的重要修正和补充。人们通过体外模拟该过程，以样本中提取的mRNA为模板，在逆转录酶的作用下，合成出互补的cDNA，构建cDNA文库，并从中筛选特异的目的基因。该方法已成为基因工程技术中最常用的获得目的基因的策略之一。
