
Bone tissue consists of osteoclasts, responsible for bone resorption, and osteoblasts and osteocytes, responsible for bone formation. These cell groups work in concert to maintain bone strength and elasticity.
Osteoblast-Inducer Reagent (for animal cell) contains a set of osteoblast differentiationreagents including hydrocortisone, beta-glycerophosphate, and ascorbic acid.
Bone tissue consists of osteoclasts, responsible for bone resorption, and osteoblasts and osteocytes, responsible for bone formation. These cell groups work in concert to maintain bone strength and elasticity.
Osteoblast-Inducer Reagent (for animal cell) contains a set of osteoblast differentiationreagents including hydrocortisone, beta-glycerophosphate, and ascorbic acid. Thesereagents induce efficient stem cell differentiation of bone marrow-derived cells and adipose-derived stem cells (mesenchymal stem cells) into osteoblasts when added to culture medium.
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2.模板浓度测定:分光光度计或NanoDrop
3.逆转录:逆转录试剂盒(或者一步法试剂盒),这一步可以用普通PCR做,也可以用水域做。
4.荧光定量PCR试剂:通常有用SYBRGreenMix做的,但是这里建议你用EvaGreen做,灵敏度和平行性都要好于SYBRGreen,并且如果你那是ABI或者Stratagene的PCR如果用SYBRGreen还需要加一步Rox很麻烦。
5.其他:除了以上的那些还需要离心管、PCR管或板(Axygen反应比较好)、移液枪等,暂时就想到这么多。
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