
DescriptionThe ViPrimePLUS Capra hircus (Goat) qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Capra hircus (Goat) genome in food samples.ViPrimePLUS Capra hircus (Goat) qPCR Kit is intended for research use only.
Kit Component
- Goat Primers and Probe Mix (Goat PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
Technology | Taqman probe-based real-time PCR assay |
Type of Nucleic Acid Kit | DNA |
Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
Sample Material | Food samples |
Gene Target | Mitochondrial DNA specific to the species |
Detection Limit | 10 to <100 copies per reaction |
Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
Controls Included | •Internal Extraction Control (IEC) as inhibition control •Positive control |
Channels | •FAM channels detect pathogen amplicons•VIC/HEX channel detects IEC amplicons |
Ordering Information
Catalog No | Description | Pack Size |
QI0010 | ViPrimePLUS Capra hircus (Goat) qPCR Kit | 100 reactions |
DownloadManual
ViPrimePLUS Capra hircus (Goat) qPCR Kit
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TAKARA的RR047A逆转录试剂盒,做逆转录程序设置错误本来应该37℃15min85℃5s,变成37℃15s,85℃
5s,请问再重新设置程序可以吗,85℃酶会全部失活吗?
加尾法是采用加A酶先对mirna进行加尾,然后再用带oligodt的引物反转录,他的反转录引物是通用的,一次反转录可以获得所有miRNA的cdna,效率高。茎环法是采用特异性反转录引物序列+颈环结构作为反转录引物进行反转录的,一次反转录只能获得一种mirna的cdna,可能有几种一起反转录的,这个我不太清楚一起反转录的效果。然后茎环法的经典即ABI的探针法定量试剂盒。我用过茎环法反转录+染料法定量的,下游引物为通用引物,结果不太好,就现在用的这家的他采用的是双向引物都是特异性的,效果还可以。不知道我说的清楚不,希望能对你有帮助。
向左转|向右转
逆转录(reverse transcription)是以RNA为模板合成DNA的过程,即RNA指导下的DNA合成。此过程中,核酸合成与转录(DNA到RNA)过程与遗传信息的流动方向(RNA到DNA)相反,故称为逆转录。逆转录过程是RNA病毒的复制形式之一,需逆转录酶的催化。 逆转录过程的揭示是分子生物学研究中的重大发现,是对中心法则的重要修正和补充。人们通过体外模拟该过程,以样本中提取的mRNA为模板,在逆转录酶的作用下,合成出互补的cDNA,构建cDNA文库,并从中筛选特异的目的基因。该方法已成为基因工程技术中最常用的获得目的基因的策略之一。
大家miRNA逆转录和qPCR的试剂盒用的是那个公司的啊,求推荐!

