The FLIPR® Membrane Potential Assay Kits deliver homogenous fluorescence-based formulations for observation of real-time membrane potential changes associated with ion channel activation and ion transporter proteins.
Each homogeneous assay kit utilizes a proprietary indicator dye and quencher combination to maximize cell line/channel/compound applicability, while eliminating causes of data variability. This unique formula responds 10 times faster and has greater temperature stability than traditional dyes, providing high quality screening data that shows good correlation with manual patch clamp assays.Because ion channel activity is highly sensitive and potentially impacted by subtle chemical changes, two FLIPR® Membrane Potential Assay Kits (Red and Blue) are available to select the optimal conditions for your delicate ion channel targets. Both formulations utilize Molecular Devices proprietary quench technology to enhance signal windows and yield acceptable Z-scores to screen a variety of targets, including TRP, ligand-, cyclic nucleotide- and voltage-gated channels.Unlike traditional dyes such as DiBAC, FLIPR Membrane Potential Assay Kit detects bidirectional gradient changes so you can monitor both variable and control conditions within a single experiment. We recommend evaluating both assay kits to discern which formulation is right for your target.
Available Configurations:
| Configuration | Description | RED Kit Part Number | BLUE Kit Part Number |
| Explorer Kit |
* Each reagent vial (Component A) is sufficient for 1 plate (96-, 384-, 1536-well). Each kit is sufficient for 10 plates. | (R8126) | (R8042) |
| Bulk Kit |
| (R8123) | (R8034) |
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加尾法是采用加A酶先对mirna进行加尾,然后再用带oligodt的引物反转录,他的反转录引物是通用的,一次反转录可以获得所有miRNA的cdna,效率高。茎环法是采用特异性反转录引物序列+颈环结构作为反转录引物进行反转录的,一次反转录只能获得一种mirna的cdna,可能有几种一起反转录的,这个我不太清楚一起反转录的效果。然后茎环法的经典即ABI的探针法定量试剂盒。我用过茎环法反转录+染料法定量的,下游引物为通用引物,结果不太好,就现在用的这家的他采用的是双向引物都是特异性的,效果还可以。不知道我说的清楚不,希望能对你有帮助。
如果是用转录出的cDNA做那跟平时就一样了。不需要特别的试剂盒、

