
We offer multiple generations of transcriptome analysis kits. The core SMART (Switching Mechanism at 5" end of RNA Template) technology employed by these legacy kits provides full-length transcript information, enabling analysis of transcript isoforms, gene fusions, point mutations, and more. While our newest generation of kits provide advantages over these legacy kits, we advise finishing ongoing projects with the same kit. For new experiments, we recommend using our most advanced solutions for single-cell RNA-seq or RNA-seq.
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2.模板浓度测定:分光光度计或NanoDrop
3.逆转录:逆转录试剂盒(或者一步法试剂盒),这一步可以用普通PCR做,也可以用水域做。
4.荧光定量PCR试剂:通常有用SYBRGreenMix做的,但是这里建议你用EvaGreen做,灵敏度和平行性都要好于SYBRGreen,并且如果你那是ABI或者Stratagene的PCR如果用SYBRGreen还需要加一步Rox很麻烦。
5.其他:除了以上的那些还需要离心管、PCR管或板(Axygen反应比较好)、移液枪等,暂时就想到这么多。
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