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TBtools基因家族分析详细教程(1)

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ProteinName

CAXII
HsT18816
CAR12
2310047E01Rik
AI314958
Ca12
carbonicanhydraseXII
carbonicanyhydrase12
CA12
Car12

ProteinCharacteristics

ExtracellularDomain,AA1-291

Origin

Human

Source

HumanCells

ProteinType

Recombinant

BiologicalActivity

Active

Purificationtag/Conjugate

Histag

Application

FunctionalStudies(Func),SDS-PAGE(SDS)

Options

Supplier

SupplierProductNo.

Productdetails ReferencesImagesHandlingApplicationDetailsTargetdetailsbacktotop
Productdetails ShowHide
Characteristics	TherecombinanthumanCA12consistingof278aminoacidsandhasacalculatedmolecularmassof31.6kDa.Asaresultofglycosylation,therecombinantproteinmigratesasanapproximately40-45kDaproteininSDS-PAGEunderreducingconditions. ProteinStructure:ADNAsequenceencodingtheextracellulardomainofhumanCA12(NP_001209.1)(Met1-Gln291)wasexpressedwithaC-terminalpolyhistidinetag. PredictedN-Term:Ala25
Purity	>97 %asdeterminedbySDS-PAGE
EndotoxinLevel	<1.0EUperμgoftheproteinasdeterminedbytheLALmethod
ProductDetails:BiologicalActivityComment	Measuredbyitsesteraseactivity.Thespecificactivityis>40pmoles/min/μg,asmeasuredwith1mM4-Nitrophenylacetateand2.5μgenzymeat400nmin100μLof12.5mMTris,75mMNaCl,pH7.5.

Targetdetails ReferencesImagesHandlingApplicationDetailsbacktotop
Targetdetails ShowHide
Background	Synonyms:CA12,CAXII,FLJ20151,HsT18816
MolecularWeight	31.6kDa,40-45kDa
NCBIAccession	NP_001209
ResearchArea	Cancer

ApplicationDetails ReferencesImagesHandlingTargetdetailsbacktotop
ApplicationNotes	Optimalworkingdilutionshouldbedeterminedbytheinvestigator.
Restrictions	ForResearchUseonly

Handling ReferencesImagesApplicationDetailsTargetdetailsbacktotop
Handling ShowHide
Format	Lyophilized
Buffer	LyophilizedfromsterilePBS, pH7.4 Normally5 %-8 %trehalose,mannitoland0.01 %Tween80areaddedasprotectantsbeforelyophilization.
HandlingAdvice	Avoidrepeatedfreeze-thawcycles.Itisrecommendedthattheproteinbealiquotedforoptimalstorage.
Storage	-20°C,-80°C
StorageComment	Storeitundersterileconditionsat-20°Cto-80°C.Itisrecommendedthattheproteinbealiquotedforoptimalstorage.Avoidrepeatedfreeze-thawcycles. Shipping:Ingeneral,recombinantproteinsareprovidedaslyophilizedpowderwhichareshippedatambienttemperature. Bulkpackagesofrecombinantproteinsareprovidedasfrozenliquid.Theyareshippedoutwithblueiceunlesscustomersrequireotherwise.
ExpiryDate	12months

Images ReferencesHandlingApplicationDetailsTargetdetailsbacktotop
Images ShowHide
SupplierImages	CA12(ExtracellularDomain),(AA1-291)(Active)protein(Histag)

References ImagesHandlingApplicationDetailsTargetdetailsbacktotop
References ShowHide
Productcitedin:	Lecoeur,Goossens,Vaccher,Bonte,Foulon:"Amultivariateapproachforthedeterminationofisoelectricpointofhumancarbonicanhydraseisoformsbycapillaryisoelectricfocusing."in:Electrophoresis,Vol.32,Issue20,pp.2857-66,2011(PubMed).
Backgroundpublications	Ivanov,Kuzmin,Wei,Pack,Geil,Johnson,Stanbridge,Lerman:"Down-regulationoftransmembranecarbonicanhydrasesinrenalcellcarcinomacelllinesbywild-typevonHippel-Lindautransgenes."in:ProceedingsoftheNationalAcademyofSciencesoftheUnitedStatesofAmerica,Vol.95,Issue21,pp.12596-601,1998(PubMed).

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发布于： 2021-09-17 阅读（93）

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ApplicationDetails ReferencesImagesHandlingTargetdetailsbacktotop
ApplicationDetails ShowHide
ApplicationNotes	Optimalworkingdilutionshouldbedeterminedbytheinvestigator.
Restrictions	ForResearchUseonly