超声破碎仪_超声破碎仪细胞颗粒释放输出功率稳定
ProductSpecification:
| AlternativeName: | Calciumrelease-activatedcalciumchannelprotein1,Transmembraneprotein142A |
| Host: | Rabbit |
| Immunogen: | Syntheticpeptidecorrespondingtoaa203-214(K203KQPGQPRPTSK214)ofhumanOrai1. |
| UniProtID: | Q96D31 |
| Speciesreactivity: | Human |
| Crossreactivity: | Doesnotcross-reactwithmouseorratOrai1. |
| Applications: | FlowCytometry,WB |
| RecommendedDilutions/Conditions: | WesternBlot(1:200) Suggesteddilutions/conditionsmaynotbeavailableforallapplications. Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| BlockingPeptide: | 40μgincluded.Lyophilized.Reconstitutewith100μldistilledwater. |
| PurityDetail: | Affinitypurified. |
| Formulation: | LyophilizedfromPBS,pH7.4,containing1%BSAand0.05%sodiumazide. |
| Reconstitution: | Reconstitutewith50μlor200μldistilledwater.Furtherdilutionsshouldbemadewithmediumcontainingacarrierproteinsuchas1%BSA. |
| Use/StABIlity: | Theantibodysolutioncanbestoredat+4°Cforupto2weeks. |
| Handling: | Avoidfreeze/thawcycles.Afterreconstitution,preparealiquotsandstoreat-20°C. |
| Shipping: | ShippedonBlueIce |
| LongTermStorage: | -20°C |
| ScientificBackground: | Cytosoliccalcium(Ca2+)haslongbeenknowntoactasakeysecondmessengerinmanyintracellularpathwaysincludingsynaptictransmission,musclecontraction,hormonalsecretion,cellgrowthandproliferation.ThemechanismcontrollingintracellularCa2+levelinfluxeitherbythecalcium-release-activatedCa2+channels(CRAC),orfromintracellularstores,hasbecomeofgreatinteresttoresearchers.Severalkeyplayersofthestoreoperatedcomplexhavebeenidentified.Orai1(alsoknownasCRACM1)actsasthestoreoperatedcalciumchannel(SOC)andSTIM1,whichactsastheendoplasmicreticulumCa2+sensor.TheformationoffunctionalchannelsbyOrai1requiresthepresenceofbothOrai1andSTIM1proteinsworkingasacomplexthatinvolvestheco-clusteringofOrai1ontheplasmamembranewithSTIM1ontheendoplasmicreticulum. |
| TechnicalInfo/ProductNotes: | Preadsorptioncontrol:1µgpeptideper1µgantibody.Prepare2tubes.Inthefirst,dilutethenecessaryamountofantibodyin200-500µlofPBS,1%BSAand0.025%sodiumazide.Preparethesecondtubeidenticallytothefirstwiththeadditionoftheappropriateamountofpeptide. Incubateatroomtemperaturefor1hr. Centrifugebothtubesat10,000Xgfor5min. Usethesupernatantsfromtubes1and2forparallelexperiments. |
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发布于 ： 2021-09-14
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