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Tuning of CD40–CD154 Interactions in Human BLymphocyte...
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ProteinName
CA12
  • CAXII
  • HsT18816
  • CAR12
  • 2310047E01Rik
  • AI314958
  • Ca12
  • carbonicanhydraseXII
  • carbonicanyhydrase12
  • CA12
  • Car12
ProteinCharacteristics
ExtracellularDomain,AA1-301
3AA25-301
2AA25-354
1AA1-291
1AA1-354
1AA154-307
1AA161-318
1AA25-124
1AA25-303
1ExtracellularDomain
1TranscriptVariant1
1fulllength
1partial
Origin
Mouse(Murine)
8Human
5Mouse(Murine)
2Rat(Rattus)
1Rabbit
Source
HumanCells
8Escherichiacoli(E.coli)
2InsectCells
2Wheatgerm
1HEK-293Cells
1HumanCells
1MammalianCells
1Yeast
ProteinType
Recombinant
BiologicalActivity
Active
Purificationtag/Conjugate
Histag
Application
FunctionalStudies(Func),SDS-PAGE(SDS)
Options
Bulkdiscount
Supplier
SupplierProductNo.
CharacteristicsThesecretedrecombinantmouseCA12consistsof288aminoacidsandhasacalculatedmolecularmassof32.8kDa.Asaresultofglycosylation,therecombinantproteinmigratesasanapproximately40-45kDaproteininSDS-PAGEunderreducingconditions.

ProteinStructure:ADNAsequenceencodingtheextracellulardomain(Met1-Ser301)ofmouseCA12(NP_848483.2)precursorwasexpressedwithaC-terminalpolyhistidinetag.
PredictedN-Term:Ala25
Purity>95 %asdeterminedbySDS-PAGE
EndotoxinLevel<1.0EUperμgoftheproteinasdeterminedbytheLALmethod
ProductDetails:BiologicalActivityCommentMeasuredbyitsesteraseactivity.Thespecificactivityis>50pmoles/min/μg,asmeasuredwith1mM4-Nitrophenylacetateand0.4μgenzymeat400nmin100μLof12.5mMTris,75mMNaCl,pH7.5.
BackgroundSynonyms:2310047E01Rik,AI314958,Car12
MolecularWeight32.8kDa,40-45kDa
NCBIAccessionNP_848483
ResearchAreaCancer
ApplicationNotesOptimalworkingdilutionshouldbedeterminedbytheinvestigator.
RestrictionsForResearchUseonly
FormatLyophilized
BufferLyophilizedfromsterilePBS, pH7.4
Normally5 %-8 %trehalose,mannitoland0.01 %Tween80areaddedasprotectantsbeforelyophilization.
HandlingAdviceAvoidrepeatedfreeze-thawcycles.Itisrecommendedthattheproteinbealiquotedforoptimalstorage.
Storage-20°C,-80°C
StorageCommentStoreitundersterileconditionsat-20°Cto-80°C.Itisrecommendedthattheproteinbealiquotedforoptimalstorage.Avoidrepeatedfreeze-thawcycles.

Shipping:Ingeneral,recombinantproteinsareprovidedaslyophilizedpowderwhichareshippedatambienttemperature.
Bulkpackagesofrecombinantproteinsareprovidedasfrozenliquid.Theyareshippedoutwithblueiceunlesscustomersrequireotherwise.
ExpiryDate12months
SupplierImages
 image for CA12 (Extracellular Domain), (AA 1-301) (Active) protein (His tag) (ABIN2007147)CA12(ExtracellularDomain),(AA1-301)(Active)protein(Histag)
BackgroundpublicationsIvanov,Kuzmin,Wei,Pack,Geil,Johnson,Stanbridge,Lerman:"Down-regulationoftransmembranecarbonicanhydrasesinrenalcellcarcinomacelllinesbywild-typevonHippel-Lindautransgenes."in:ProceedingsoftheNationalAcademyofSciencesoftheUnitedStatesofAmerica,Vol.95,Issue21,pp.12596-601,1998(PubMed).

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