Acetylome - Deacetylase Microarray

Product Code: AT-MA_01

Peptide microarray displaying 5599 peptides derived from reported human acetylation sites (plus 384 control peptides). All 5599 peptides carry acetylated lysine residues. Readout is performed by differential measurement, allowing comparative analysis in absence (control) and presence of deacetylase (or other) activities. A control microarray is provided with each order.Amount: 1 peptide microarray (each peptide is deposited 1x per subarray, 3 subarrays per slide) + 1 control microarray per order +dummy slides and spacers (needed for manual incubation) Purity: Free of truncated sequencesDelivery Format: Peptides immobilized onto glass slide (3 x 1 inches; 75 by 25 mm)Application(s): Binding experiments, Substrate identification, Acetyltransferase assays, Acetyltransferase inhibitor screens Indication(s)/Topic(s): Cancer, Acetyltransferase, Histones, Gene regulation and expression, Epigenetics, Cell signaling, Acetylome, Deacetylase, MethyltransferaseDelivery Time: 2-5 days

Acetyltransferase & Deacetylase Microarrays JPT Peptide Technologies applies its unique peptide microarray platform to generate peptide microarrays on glass slides for characterization of enzymes such as acetyltransferases, methyltransferases and deacetylases. Peptides are immobilized on glass slides via a flexible linker. Chemoselective coupling generates microarrays displaying covalently attached peptides. Multiple identical copies of each microarray are produced. Incubation can be performed manually or automated. Read-out via fluorescence results in low background and high sensitivity.

Benefits of JPTs Acetyltransferase & Deacetylase Microarrays - Unique collection of all identified human acetylation sites - Get hundreds of identical microarray copies - Peptides are free of truncated sequences - Small amounts of your precious samples are needed for incubation - Applicable to a variety of biological samples (e.g. serum, lysate, phosphatase)- Low background on glass surface. Read-out via fluorescence - Save time for HTS assay setup - Screen thousands of potential peptide substrates economically

Acetylome - Deacetylase Microarray

Selected References\"An Acetylome Peptide Microarray Reveals Specificities and Deacetylation Substrates for all Human Sirtuin Isoforms\" Rauh et al, Nature Communications (2013) - PMID: 23995836\"Exploring the human acetylome using high-density peptide microarrays\"Masch et al., Application Note (2012) (full text)

Application Notes for Enzyme Substrate Microarrays\"Exploring the human acetylome using high-density peptide microarrays\"Masch et al., Application Note (2012) (full text)\"Unraveling Phosphorylation of the Splicing Factor SC35 by the Dual Signaling Kinase PKC in Human T-Cells\" Rao et al., Application Note (2016) (full text)\"Use of High-Density Histone Peptide Arrays for Parsing the Specificity of a Histone Modifying Enzyme Complex\" Wilczek et al., Application Note (2013) (full text)

Testimonial \"We have successfully applied JPTs peptide microarrays as a screening tool for potential kinases transfering phosphate to phosphorylation sites identified by mass spectrometry. The triplicate alignment as well as the thorough selection of controls enabled generation of high quality data confirming our in vivo data generated by means of affinity chromatographic procedures.\" Marcelo P. Coba, PhD, The Wellcome Trust Sanger Institute, Cambridge, UK

Acetylome - Deacetylase Microarray