From large cells to nuclei: Key considerations for everyday sorting... Register Now Help over 2 million scientists like you save time, money & resources Be the first to review brand new products Exclusive perks, regular competitions, prizes and member-only events Give vital feedback to manufacturers & help shape new technologies PathHunter™ Protein Translocation Assays by DiscoveRx CorporationManufacturer DiscoveRx CorporationBe the first to review this productOverview and Description PathHunter™ Protein Translocation Assays are single-addition, homogeneous assays that measure the effects of agonist or antagonist compounds on target protein translocation in vivo to the nucleus. The assays facilitate cost-effective, high throughput screening, because they are simple, use microtitre plates, and do not require imaging or antibodies. A PathHunter CHO-K1 Cell Line expressing a protein known to translocate to the nucleus, such as the glucocorticoid receptor... Read more PathHunter™ Protein Translocation Assays Reviews Description News & Articles0 Scientists have reviewed this product Write the First Review No Reviews Overview and Description PathHunter™ Protein Translocation Assays are single-addition, homogeneous assays that measure the effects of agonist or antagonist compounds on target protein translocation in vivo to the nucleus. The assays facilitate cost-effective, high throughput screening, because they are simple, use microtitre plates, and do not require imaging or antibodies. A PathHunter CHO-K1 Cell Line expressing a protein known to translocate to the nucleus, such as the glucocorticoid receptor, is used together with the PathHunter EFC Detection Reagent Kit for chemiluminescent read out.

Ready-to-go translocation assays include: • AR Translocation• GR Translocation• LXRalpha Translocation• NFAT Translocation• VDR Translocation

Customer services are offered on request.Features and Benefits» In vivo Whole Cell Assay • Provides a high-throughput protein translocation assay in a simple, cost-effective format » No Imaging, Antibodies, or GFP • Reduces assay development, data analysis and data storage » No Fluorescent Interference • Eliminates optical interference from fluorescent compounds by chemiluminescent format » Homogeneous • Provides mix and read protocols to facilitate assay development and automation » Convenient • Uses standard microplates, existing liquid handlers, and a wide range of luminometers, mutlimode microplate readers or CCD devices. Measuring Protein Translocation in Whole Cells using PathHunter EFC Detection Ready-to-use Biosensor Cell Lines stably express the Enzyme Acceptor (EA) ß-galactosidase (ß-gal) fragment exclusively in the cell nucleus and the target of interest in the cytoplasm as a ProLabel fusion protein. Upon translocation of the cytoplasmic protein to the nucleus, ß-gal activity is restored and a chemiluminescent signal is generated. Kit Contents » Double-stable PathHunter Cell Line for a specific target » PathHunter EFC Detection Reagents purchased separately Instrument Requirements » Luminometer, Multimode Microplate Reader or CCD Camera

Product NewsDiscoveRx announces a revolutionary application for their PathHunter™ technology platform, winner of the 2006 Frost & Sullivan Technology Innovation Award01 Sep 2006Product NewsDiscoveRx PathHunter™ Technology Successfully Implemented in NIH Roadmap Initiative22 Aug 2006 Why request a quote through SelectScience?

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