Excellgen/Thermus aquaticus Muts DNA mismatch repair protein, Taq-Muts, no tag/EG-25/1 mg
| Product Name | Thermus aquaticus MutS DNA mismatch repair protein |
| Description | The Taq MutS DNA mismatch protein recognizes heteroduplex DNAs containing mispaired or unpaired bases. This Muts protein binds in vitro to heteroduplex DNAs containing mispaired or unpaired bases over a wide temperature range from 4 to 70 °C and has a thermostable ATPase activity. This thermostable Taq MutS is active at temperature between 0 to 75°C. Since Taq MutS efficiently binds to 1-4 bases deletion (or insertion) and mismatch base pairs of GT, CT and AG, it is useful for detecting these mutations. Mutations can be detected in polyacrylamide gels |
| Applications | - Remove mismatch DNA (error correction) from gene synthesis reaction
- Mutation detection and removal
- Rapid isothermal SNP detection
|
| Source | E. coli |
| Fusion Tag | None |
| Purification Method | FPLC |
| Concentration | 1 mg/ml |
| Purity | ~ 95% as determined by SDS-PAGE |
| Accession # | AAC43637, TAU3311, U33117 |
| Gene Name | Thermus aquaticus MutS DNA mismatch repair protein |
| Protein Sequence | 1 MEGMLKGEGP GPLPPLLQQY VELRDQYPDY LLLFQVGDFY ECFGEDAERL ARALGLVLTH61 KTSKDFTTPM AGIPLRAFEA YAERLLKMGF RLAVADQVEP AEEAEGLVRR EVTQLLTPGT121 LLQESLLPRE ANYLAAIATG DGWGLAFLDV STGEFKGTVL KSKSALYDEL FRHRPAEVLL181 APELLENGAF LDEFRKRFPV MLSEAPFEPE GEGPLALRRA RGALLAYAQR TQGGALSLQP241 FRFYDPGAFM RLPEATLRAL EVFEPLRGQD TLFSVLDETR TAPGRRLLQS WLRHPLLDRG301 PLEARLDRVE GFVREGALRE GVRRLLYRLA DLERLATRLE LGRASPKDLG ALRRSLQILP361 ELRALLGEEV GLPDLSPLKE ELEAALVEDP PLKVSEGGLI REGYDPDLDA LRAAHREGVA421 YFLELEERER ERTGIPTLKV GYNAVFGYYL EVTRPYYERV PKEYRPVQTL KDRQRYTLPE481 MKEKEREVYR LEALIRRREE EVFLEVRERA KRQAEALREA ARILAELDVY AALAEVAVRY541 GYVRPRFGDR LQIRAGRHPV VERRTEFVPN DLEMAHELVL ITGPNMAGKS TFLRQTALIA601 LLAQVGSFVP AEEAHLPLFD GIYTRIGASD DLAGGKSTFM VEMEEVALIL KEATENSLVL661 LDEVGRGTSS LDGVAIATAV AEALHERRAY TLFATHYFEL TALGLPRLKN LHVAAREEAG721 GLVFYHQVLP GPASKSYGVE VAAMAGLPKE VVARARALLQ AMAARREGAL DAVLERLLAL781 DPDRLTPLEA LRLLQELKAL ALGAPLDTMK G |
| Storage Buffer | 20 mM Tris-HCl, pH 8.0, 250 mM NaCl, 1 mM DTT, 50% Glycerol |
| Reaction Buffer | 100mM KCl, 50 mM Tris-HCl, pH 8.5, 5~20 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 2% Glycerol, 65 °C |
| Storage | -20 to -80 °C. |
| Shipping | 4°C or dry ice |
| References | Protein-mediated error correction for de novo DNA synthesis. Nucleic Acids Res. 2004 Nov 23;32(20):e162.Correcting errors in synthetic DNA through consensus shuffling. Nucleic Acids Res. 2005 Mar 30;33(6):e55.MutS as a tool for mutation detection. Acta Biochim Pol. 2005;52(3):575-83. Epub 2005 Aug 4.One tube mutation detection using sensitive fluorescent dyeing of MutS protected DNA. Nucleic Acids Res. 2000 Apr 15;28(8):E36.Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology. Nature Methods - 4, 257 - 262 (2007) doi:10.1038/nmeth1007 |
本文链接： https://www.ebiomall.cn/b120-excellgen/info-1546735404.html
免责声明 本文仅代表作者个人观点，与本网无关。其创作性以及文中陈述文字和内容未经本站证实，对本文以及其中全部或者部分内容、文字的真实性、完整性、及时性本站不做任何保证或承诺，请读者仅作参考，并请自行核实相关内容。
版权声明 未经蚂蚁淘授权不得转载、摘编或利用其他方式使用上述作品。已经经本网授权使用作品的，应该授权范围内使用，并注明“来源：蚂蚁淘”。违反上述声明者，本网将追究其相关法律责任。
