Epigentek/EpiNext NGS Barcode (Index) Set-12/P-1060-144/144 reactions
| Input Type: | DNA |
| Research Area: | Next Gen Sequencing |
| Target Application: | Library Construction |
| Vessel Format: | Columns/Tubes |
| 100% Guarantee: | 6 months |
Product Overview
The EpiNext™ NGS Barcode (Index) Set-12 is designed to construct multiplex DNA/RNA libraries used for next-generation sequencing with the Illumina platform including GAIIx, HiSeq, MiSeq, and NextSeq.This product includes 12 DNA barcodes, each of which contain an index and flow cell binding sequence and can be attached to the sample insert during library preparation. Multiplexing several samples in a single flow cell can be achieved by pooling with the EpiNext™ NGS Barcodes. Pooling barcoded samples into a single flow cell significantly reduces hands-on time and provides robust data quality in NGS. The EpiNext™ NGS Barcode (Index) Set-12 has the following features: - The barcodes contained in the EpiNext™ NGS Barcode (Index) Set-12 can be used with single, paired-end and multiplex reads and are compatible with various library preparation workflows used for Illumina NGS, including DNA-seq, RNA-seq, ChIP-seq, MethylC-seq, MeDIP-seq, hMeDIP-seq, DNA bisulfite-seq, oxBS-seq and RNA bisulfite-seq.
- The barcodes are also optimized for use with Epigentek’s EpiNext™ NGS library preparation kit series including EpiNext™ DNA Library Preparation Kit (Illumina), EpiNext™ High-Sensitivity DNA Library Preparation Kit(Illumina), EpiNext™ Post-Bisulfite DNA Library Preparation Kit(Illumina), EpiNext™ Bisulfite-Seq High-Sensitivity Kit(Illumina), EpiNext™ ChIP-Seq High-Sensitivity Kit(Illumina), EpiNext™ RNA Bisulfite-Seq Kit and EpiNext™ MeDIP-Seq Kit.
- High throughput and cost reduction: Up to 144 multiplexed samples can be processed, which allows multiplexing of 2-12 fragment library samples on a single sequencing flow cell resulting in considerable reduction of per-sample cost.
- The indices are designed to be error resistant and allow for proper differentiation between samples.
- Minimizes PCR amplification-based bias and prevents poor reads from single base errors introduced during PCR.
| ![](\"https://www.ebiomall.com/images/Epigentek/201710/p1060schematic.gif\") ![](\"https://www.ebiomall.com/images/Epigentek/201710/arrow_up.gif\") Fig. 1.Workflow of the DNA library preparation with the EpiNext™ NGS Barcode (Index) Set-12.
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Product Components
EpiNext™ Barcodes 1-12 (10 µM)*†EpiNext™ Universal Primer (10 µM)*User Guide
* Spin the solution down to the bottom prior to use.
†The EpiNext™ NGS Barcode Set-12 contains 12 DNA barcodes with sufficient volume for 12 reactions each, for a total of 144 reactions. The shelf life of each reagent is 6 months when stored at -20°C.
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