DESCRIPTION:

A fully validated, highly specific, affinity purified rabbit polyclonal antibody to GFP, EGFP, PA-GFP, CFP and YFP

Background: The green fluorescent protein (GFP) is composed of 238 amino acid residues (26.9kDa) and exhibits bright green fluorescence when exposed to ultraviolet blue light. It has become a well established reporter for gene expression and marker for protein targeting. Subtle modifications have been made to the sequence of GFP improving its solubility and photostablility (eGFP) to allow acquisition of more complex data (FRET, FRAP). Moreover, subtle residue modifications have been engineered to alter the emission wavelength such as in CFP, YFP to facilitate duel labelling. Anti-GFP Ab is a polyclonal antibody raised in rabbit by immunisation with, and affinity purification against full length GFP (Faix et al., 2001). It produces exceptional staining for GFP, PA-GFP, EGFP, CFP and YFP in western blotting at a dilution of 1:10,000 and bioimaging applications at high dilutions, it has featured in an impressive number of publications.

Tested Applications: Western blot (1:10,000 dilution), IF Microscopy (1:500 - 5000 dilution)

DATA SHEET:

Description	Lyophilised rabbit polyclonal monoclonal antibody to GFP
Immunogen	Recombinant GFP together with complete Freunds adjuvant
Isotype	IgG
Purification	Affinity purified using purified GFP covalently coupled to cyanogen bromide activated agarose.
Species reactivity	Will recognise regular, enhanced and photo activatable green fluorescent proteins GFP, EGFP, PAGFP. It will also recognise cyan and yellow fluorescent proteins CFP, YFP, but will not recognise any red fluorescent proteins.
Tested applications	WB, IF, IP, ELISA
Recommended dilution	WB 1:10,000, IF 1:500-5000, IP, ELISA
Optimization	Optimal dilutions to be determined by end user
Storage	Lyophilised antibody is stable at 4 °C when stored with desiccant. Reconstitute lyophilised powder in 6.25 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles.
Storage	Lyophilised: 4 °C. Reconstituted:-80 °C
Datasheet	A010-pGFP-50
MSDS	A010-pGFP-50
Regulatory statement	Product for research use only. Not intended for diagnostic or therapeutic use.