DESCRIPTION:

A fully validated, highly specific affinity purified rabbit antibody to threonine-17 phosphorylated forms of phospholamban

Phospholamban (PLB/PLN) is a small transmembrane protein which plays an important role in controlling the activity of the sarcoplasmic reticulum ATPase (SERCA2a) of cardiac muscle during calcium sequestration.

Phospholamban is phosphorylated on separate amino acid residues by cAMP-dependent, and cGMP-dependent (serine-16)and Ca²⁺/CaM-dependent (threonine-17) protein kinases in response to β-adrenergic stimulation. Akt has also been shown to phosphorylate Thr-17. The result is an increased calcium pump activity which reduces the time course of the calcium transient, increases the calcium load in the sarcoplasmic reticulum, and consequently, produces a larger calcium transient at the next action potential. However, alteration in this homeostatic interaction has been shown to result in heart failure.

Tested applications: Western blot (1:5000 dilution). Supplied as lyophilised affinity purified IgG.

DATA SHEET:

Description	Affinity purified Rabbit polyclonal antibody (A010-13AP) to phospholamban phospho Thr-17
Immunogen	Synthetic peptide (R9SAIRRAST(PO3H2)IEY20) corresponding to amino acids surrounding the phosphorylated threonine residue at position 17 of phospholamban, which was conjugated to keyhole limpet hemocyanin (KLH)
Isotype	IgG
Purification	Protein G affinity purified. Eluted in citrate buffer pH2.8, neutralised with Tris-base. Stabiliser added to 20%v/v
Species reactivity	The antibody recognises mono and oligomeric phospholamban when phosphorylated at threonine-17. Binding of the antibody to its target epitope is blocked in the presence of a phosphopeptide containing the PLB Phospho Thr-17 epitope.
Tested applications	WB
Recommended dilution	WB 1:1000
Optimization	Optimal dilutions to be determined by end user
Storage	Lyophilised antibody is stable at 4°C when stored with desiccant. Reconstitute lyophilised powder in 100 µl of 18 MΩ H2O, aliquot and store frozen at -80°C for 1 year. Avoid freeze – thaw cycles.
Storage	Lyophilised: 4 °C Reconstituted:-80 °C
Datasheet	A010-13AP
MSDS	A010-13AP
Regulatory statement	Product for research use only. Not intended for diagnostic or therapeutic use.