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Biosensis/Human NGF/NGF beta ELISA Kit (2 plates)/BEK-2075-2P/1 Ea
2026-02-09
| Find a 1 plate size of this product here. Enquire about bulk discounts for ten or more plates, at sales@biosensis.com | |
| Description | For a faster assay without compromising on sensitivity, try our NGF Rapid ELISA kit (BEK-2212-2P)!![\"Learn](\"https://www.ebiomall.com/images/learn-more.jpg\") The human NGF Kit is a sandwich ELISA. The capture antibody is a monoclonal human NGF antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known NGF concentration are added to these wells and allowed to complex with the bound NGF antibody. A biotinylated human NGF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of NGF present in the samples. The purpose of this kit is the in-vitro quantitative determination of human NGF in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
| Batch No. | See product labels |
| Antigen | FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. |
| Other Names | Nerve growth factor; beta-NGF; |
| Accession | P01138 NGF_HUMAN; |
| Specificity | Human NGF/NGF beta |
| Cross-reactivity | The affinity purified antibodies used in this elisa react with human NGF and show some cross reactivity to mouse/rat NGF. Cross reactivity is approximately 30% |
| Storage | Store at 4°C |
| Kit components | The ELISA kit box contains 2 x 96 pre-coated strip plates, protein standards, detection reagents, substrate buffer and precise instructions. |
| Range | 15.6 pg/ml - 1,000 pg/ml |
| Sensitivity | < 1 pg/ml |
| Kit protocol | ![\"Click](\"https://www.ebiomall.com/images/pdf1.gif\" "\\"") BEK-2075-2P_as_at_July2016_protocol.pdf |
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