Biosensis/Neurotrophin Antibody Kit/KIT-NT-500/5 vials, each containing 500 μg sheep IgG-蚂蚁淘商城

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Biosensis/Neurotrophin Antibody Kit/KIT-NT-500/5 vials, each containing 500 μg sheep IgG

2026-02-09

Description	Neurotrophins are growth factors that regulate neuronal development, maintenance and plasticity. These homo-dimeric proteins activate a multitude of intracellular signalling cascades through their interaction with Trk receptors and p75NTR. The Neurotrophin Antibody Kit (Trial Size) contains a collection of Biosensis popular sheep antibodies raised against BDNF (S-015-500), NGF (S-052-500), NT3 (S-057-500) and NT4/5 (S-059-500). The kit is completed with one vial of normal sheep IgG (S-1754-100) for use as negative control. This full size version of Biosensis Neurotrophin Antibody Kit contains 500 μg sheep IgG of each antibody.This kit presents a cost-effective way to investigate neurotrophin protein expression and function by immunological techniques such as immunohistochemistry, ELISA, and inhibition of biological activity.
Related products	S-015-500, Sheep polyclonal antibody to rhBDNFS-052-500, Sheep polyclonal antibody to beta NGFS-057-500, Sheep polyclonal antibody to rhNT3S-059-500, Sheep polyclonal antibody to rhNT4/5S-1754-500, Normal sheep IgG from non-immunized animal
Batch No.	See product label.
Unit size	5 vials, each containing 500 μg sheep IgG
Antigen	BDNF: recombinant human BDNFNGF: native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)NT3: recombinant human NT3NT4/5: recombinant human NT4
Antibody Type	Polyclonal.
Isotype	IgG
Accession	P23560 BDNF_HUMAN; P01139 NGF_MOUSE;P20783 NTF3_HUMAN;P34130 NTF4_HUMAN;
Produced in	Sheep.
Purity	Protein G purified IgG.
Applications	Immunohistochemistry: 1-10 µg/mL. BDNF antibody works best on Zambonis fixed, frozen tissue, and is not recommended for paraffin-embedded tissue.ELISA: 1-10 µg/mL.Western Blotting: 1-10 µg/mL. Western blotting is not recommended for the BDNF antibody.Inhibition of biological activity in vitro/in vivo: Expected ED50 (in vivo) values are in the range of 2-10 µg/mL. The NGF antibody completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro.Biosensis recommends optimal dilutions/concentrations should be determined by the end user. The negative control (normal sheep IgG) should be used at the same concentration as the neurotrophin antibodies.
Specificity	BDNF Antibody: A cross-reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by ELISA.NGF Antibody: A cross-reactivity of less than 1% against recombinant human BDNF, NT3 and NT4/5 by ELISA has been shown.NT3 Antibody: A cross-reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by ELISA.NT4/5 Antibody: Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot.
Species Against	Human (BDNF, NT3 and NT4/5). Mouse (NGF).
Cross-reactivity	Neurotrophins share a large degree of homology among human, rodent and other mammalian species, thus these neurotrophin antibodies are useful for a number of species, including human, mouse and rat.
Form	Lyophilized from PBS, pH 7.4, without preservatives.
Appearance	White powder.
Reconstitution	Reconstitute each vial with 500 µL sterile-filtered, ultrapure water, to achieve an antibody concentration of 1 mg/mL. Centrifuge briefly to remove any insoluble material.
Storage	Store lyophilised, unopened vials at 2- 8°C or lower. After reconstitution, prepare aliquots and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date	12 months after purchase (unopened vials).